Veterinary Medicine Commons^™

Short-Term Transport Stress And Supplementation Alter Immune Function In Aged Horses, Ashton B. Miller, Patricia A. Harris, Virginia D. Barker, Amanda A. Adams

Veterinary Science Faculty Publications

Long-distance transport is associated with stress-related changes in equine immune function, and shipping-associated illnesses are often reported. Horses are frequently transported short distances, yet the effects of short-term transport on immune function remain largely unknown. Twelve horses, aged 15–30 yr, were assigned to either the control (n = 6) or treatment (n = 6) groups; treatment horses received a daily antioxidant supplement 3 weeks before and after transport. All horses were transported for approximately 1.5–2 hr on Day 0. Blood was collected via jugular venipuncture at 15-min pre- and post-transport and on Days –21, 1, 3, 7, 14, and 21. …

Continuous Low-Intensity Ultrasound Attenuates Il-6 And Tnfα-Induced Catabolic Effects And Repairs Chondral Fissures In Bovine Osteochondral Explants, Neety Sahu, Hendrik J. Viljoen, Anuradha Subramanian

Department of Chemical and Biomolecular Engineering: Faculty Publications

Background: Cartilage repair outcomes are compromised in a pro-inflammatory environment; therefore, the mitigation of pro-inflammatory responses is beneficial. Treatment with continuous low-intensity ultrasound (cLIUS) at the resonant frequency of 5 MHz is proposed for the repair of chondral fissures under pro-inflammatory conditions.

Methods: Bovine osteochondral explants, concentrically incised to create chondral fissures, were maintained under cLIUS (14 kPa (5 MHz, 2.5 Vpp), 20 min, 4 times/day) for a period of 28 days in the presence or absence of cytokines, interleukin-6 (IL-6) or tumor necrosis factor (TNF)α. Outcome assessments included histological and immunohistochemical staining of the explants; and the expression of …

Role Of An Iron-Dependent Transcriptional Regulator In The Pathogenesis And Host Response To Infection With Streptococcus Pneumoniae., Radha Gupta, Minny Bhatty, Edwin Swiatlo, Bindu Nanduri

College of Veterinary Medicine Publications and Scholarship

Iron is a critical cofactor for many enzymes and is known to regulate gene expression in many bacterial pathogens. Streptococcus pneumoniae normally inhabits the upper respiratory mucosa but can also invade and replicate in lungs and blood. These anatomic sites vary considerably in both the quantity and form of available iron. The genome of serotype 4 pneumococcal strain TIGR4 encodes a putative iron-dependent transcriptional regulator (IDTR). A mutant deleted at idtr (?idtr) exhibited growth kinetics similar to parent strain TIGR4 in vitro and in mouse blood for up to 48 hours following infection. However, ?idtr was significantly attenuated in a …

Inflammatory Responses To Induced Infectious Endometritis In Mares Resistant Or Susceptible To Persistent Endometritis, Mette Christoffersen, Elizabeth Woodward, Anders M. Bojesen, Stine Jacobsen, Morten R. Petersen, Mats H. T. Troedsson, Henrik Lehn-Jensen

Veterinary Science Faculty Publications

BACKGROUND: The objective of the study was to evaluate the gene expression of inflammatory cytokines (interleukin [IL]-1β, IL-6, IL-8, IL-10, tumor necrosis factor [TNF]-α, IL-1 receptor antagonist [ra] and serum amyloid A (SAA) in endometrial tissue and circulating leukocytes in response to uterine inoculation of 105 colony forming units (CFU) Escherichia coli in mares. Before inoculation, mares were classified as resistant or susceptible to persistent endometritis based on their uterine inflammatory response to infusion of 109 killed spermatozoa and histological assessment of the endometrial quality. Endometrial biopsies were obtained 3, 12, 24 and 72 hours (h) after bacterial inoculation and …