Veterinary Medicine Commons^™

An Evaluation Of A Liquid Antimicrobial (Sal Curb®) For Reducing The Risk Of Porcine Epidemic Diarrhea Virus Infection Of Naïve Pigs During Consumption Of Contaminated Feed, Scott Dee, Casey Neill, Travis Clement, Jane Christopher-Hennings, Eric A. Nelson

Veterinary and Biomedical Sciences Faculty Publications

Background: Since its initial detection in May 2013, porcine epidemic diarrhea virus (PEDV) has spread rapidly throughout the US swine industry. Recently, contaminated feed was confirmed as a vehicle for PEDV infection of naïve piglets. This research provides in vivo data supporting the ability of a liquid antimicrobial product to reduce this risk.
Results: Sal CURB® (Kemin Industries, Des Moines, IA, USA) is a FDA-approved liquid antimicrobial used to control Salmonella contamination in poultry and swine diets. To test its effect against PEDV, Sal CURB®-treated feed was spiked with a stock isolate of PEDV (Ct = 25.22), which PEDV-naïve piglets …

An Evaluation Of Contaminated Complete Feed As A Vehicle For Porcine Epidemic Diarrhea Virus Infection Of Naïve Pigs Following Consumption Via Natural Feeding Behavior: Proof Of Concept, Scott Dee, Travis Clement, Adam Schelkopf, Joel Nerem, David Knudsen, Jane Christopher-Hennings, Eric A. Nelson

Veterinary and Biomedical Sciences Faculty Publications

Background: Since its initial detection in May 2013, porcine epidemic diarrhea virus (PEDV) has spread rapidly throughout the US swine industry. Initially, contaminated feed was proposed as a risk factor for PEDV; however, data were not available to support this theory. Here we provide proof of concept of this risk by describing a novel means for recovering PEDV-contaminated complete feed material from commercial swine sites and conducting an in vivo experiment to prove its infectivity.

Results: For on-farm detection of PEDV RNA in feed, paint rollers were used to collect material from at-risk feed bins from 3 clinically affected breeding …

Characterization Of A Novel Influenza Virus In Cattle And Swine: Proposal For A New Genus In The Orthomyxoviridae Family, Ben M. Hause, Emily A. Collin, Runxia Liu, Bing Huang, Zizhang Sheng, Wuxun Lu, Dan Wang, Eric A. Nelson, Feng Li

Veterinary and Biomedical Sciences Faculty Publications

We have recently reported the isolation of a novel virus, provisionally designated C/swine/Oklahoma/1334/2011 (C/OK), with 50% overall homology to human influenza C viruses (ICV), from a pig in Oklahoma. Deep RNA sequencing of C/OK virus found a matrix 1 (M1) protein expression strategy that differed from that of ICV. The novelty of C/OK virus prompted us to investigate whether C/OK virus could exist in a nonswine species. Significantly, we found that C/OK virus was widespread in U.S. bovine herds, as demonstrated by reverse transcription (RT)-PCR and serological assays. Genome sequencing of three bovine viruses isolated from two herds in different …