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Full-Text Articles in Veterinary Medicine
Molecular Characterization Of Fecundity-Related Gene Regions In Some Of Türkiye’S Native Sheep Breeds, Ramazan Aymaz, Fulya Özdi̇l, Yalçın Yaman
Molecular Characterization Of Fecundity-Related Gene Regions In Some Of Türkiye’S Native Sheep Breeds, Ramazan Aymaz, Fulya Özdi̇l, Yalçın Yaman
Turkish Journal of Veterinary & Animal Sciences
In this study, the BMP15 and GDF9 gene regions were examined through DNA sequencing in some native sheep breeds of Türkiye (Kıvırcık, Karacabey Merino, Chios, Gökçeada, Çine Çaparı, Awassi, and Karakacan). In the BMP15 exon 2 region, two single nucleotide polymorphisms (SNPs) were identified: one synonymous SNP, P248P (proline/proline), and one nonsynonymous SNP, L251P (leucine/proline), resulting in a missense mutation. In the GDF9 exon 1 region, only a nonsynonymous SNP, R87H (histidine/ arginine), was found. In the GDF9 exon 2 region, four SNPs were identified: two nonsynonymous SNPs, E241K (glutamate/lysine) and V332I (valine/isoleucine), and two synonymous SNPs, L159L (leucine/leucine) and …
The Effect Of Saccharomyces Cerevisiae As A Probiotic On The Nutrient Degradability Of Some Commonly Feedstuffs Used In Turkey, Muslimo Abdullahi Sheikh Hassan, Mehmet Aki̇f Karsli
The Effect Of Saccharomyces Cerevisiae As A Probiotic On The Nutrient Degradability Of Some Commonly Feedstuffs Used In Turkey, Muslimo Abdullahi Sheikh Hassan, Mehmet Aki̇f Karsli
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to evaluate the effects of Saccharomyces cerevisiae as a probiotic on in situ ruminal DM, OM, CP and starch degradability values in some energy and protein sources commonly used in ruminant nutrition. In this study, the in situ degradation kinetics and fractions of dry matter, organic matter, crude protein, and starch of 6 different feedstuffs (barley, wheat, corn, sunflower seed meal, cottonseed meal and soybean meal) commonly used in animal nutrition were determined. Three ruminally cannulated Bafra sheep were used in the experiment. The experiment was designed as carryover experimental design with two periods. …
Anatomical And Histological Structures Of Eye And Lacrimal Gland In Norduz Andmorkaraman Sheep, Semi̇ne Dalga, Serap İlhan Aksu, Kadi̇r Aslan, Turgay Deprem, Reşi̇t Uğran
Anatomical And Histological Structures Of Eye And Lacrimal Gland In Norduz Andmorkaraman Sheep, Semi̇ne Dalga, Serap İlhan Aksu, Kadi̇r Aslan, Turgay Deprem, Reşi̇t Uğran
Turkish Journal of Veterinary & Animal Sciences
The current study was conducted to determine the morphological, morphometric, and histological characteristics of bulbus oculi and lacrimal gland in sheep. For this purpose, 28 pairs (n = 7 males and n = 7 females) of bulbus oculi from Morkaraman and (n = 7 males and n = 7 females) Norduz sheep were analyzed. The bulbus oculi in the skulls were dissected from the orbit and removed. The morphometric data such as distance between endpoints of the dissected bulbus oculi in temporal, nasal, and dorsal directions, the length between endpoints of the pupil in dorsal and ventral directions, and as …
Some Hematological Parameters And Enzyme Histochemistry Of Peripheral Bloodleukocytes In Kivircik Sheep, Mehmet Faruk Aydin, İhsan Kisadere
Some Hematological Parameters And Enzyme Histochemistry Of Peripheral Bloodleukocytes In Kivircik Sheep, Mehmet Faruk Aydin, İhsan Kisadere
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to determine some hematological parameters (RBC, WBC, HCT, HGB, MCH, MCHC, MCV, and PLT), the presence and percentages of ANAE and AcP-ase positivities in leukocytes and the differential leukocyte count in Kivircik sheep. Blood samples were taken from the jugular vein of 40 (rams = 20; ewes= 20) healthy, 1-year-old sheep. MCH and MCHC values were found statistically higher in rams than ewes (p < 0.05). Mean percentages of ANAE-positive PBL were defined as 74.16 ± 11.94% in ewes and 70.33 ± 8.39% in rams. It was not determined any significant changes according to sex. On the other hand, the percentages of ACP were determined as 67.12 ± 6.54% in ewes and 66.71 ± 6.58% in rams. It was not found any significant differences according to sex. For ANAE enzyme activity, lymphocytes, monocytes, neutrophils, and eosinophilic granulocytes showed a positive reaction. For ACP enzyme activity, although lymphocytes presented positivity, monocytes, neutrophils, eosinophils were negative. The ratio of lymphocyte, monocyte, neutrophile, eosinophile, and basophile of Kivircik ewes and rams were found as 64.35 ± 5.74%, 60.64 ± 8.85%; 3.41 ± 1.27%, 3.82 ± 1.42%; 27.58 ± 5.46%, 30.94 ± 8.71%; 2.41 ± 0.93%, 2.88 ± 1.45%; 1.76 ± 0.75%, 1.29 ± 0.58%, respectively. The difference between mean values was not found significant according to sex (p > 0.05). In conclusion, our study showed that enzyme histochemical methods can be used for identifying peripheral blood cells and can provide facilitates for early diagnosis of immune system-related disorders …
Relationships Between Famacha Scores And Some Blood Values In Sheep And Goats, Özcan Şahi̇n, İbrahi̇m Ayteki̇n, Sai̇m Boztepe, İsmai̇l Keski̇n, Ali̇ Karabacak, Yasi̇n Altay, Mervan Bayraktar
Relationships Between Famacha Scores And Some Blood Values In Sheep And Goats, Özcan Şahi̇n, İbrahi̇m Ayteki̇n, Sai̇m Boztepe, İsmai̇l Keski̇n, Ali̇ Karabacak, Yasi̇n Altay, Mervan Bayraktar
Turkish Journal of Veterinary & Animal Sciences
In this study, Akkaraman sheep and Hair goats raised extensively on the same farm were scored with the FAMACHA card. Score 1 was not present in both sheep and goats, whereas scores 5 and 2 were not detected in sheep and goats, respectively. While the number of animals with scores of 2, 3, and 4 was 9, 46, and 55 in sheep, the number of animals with 3, 4, and 5 scores in goats was 6, 55, and 41, respectively. In the study, the differences in the amount of RBC, Hct, and Hb in goats according to these score groups …
A Research On Babesia And Theileria Species In Sheep And Goats Of Kırıkkale Provincethrough Molecular Methods, Sami̇ Gökpinar, Aycan Nuri̇ye Gazyağci, Meral Aydeni̇zöz, Ufuk Kaya
A Research On Babesia And Theileria Species In Sheep And Goats Of Kırıkkale Provincethrough Molecular Methods, Sami̇ Gökpinar, Aycan Nuri̇ye Gazyağci, Meral Aydeni̇zöz, Ufuk Kaya
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to determine presence and prevalence of Babesia and Theileria species in the sheep and goats of stock farming in the province of Kırıkkale, which is in Central Anatolia Region of Turkey through microscopic examination and RLB method. Venous blood samples of 3 mL were collected from vena jugularis of 300 sheep and 100 goats into tubes containing EDTA. Blood smears were prepared from samples; and stained by Giemsa and examined for Theileria and Babesia under a light microscope. Genomic DNAs were extracted from blood samples with EDTA through suitable methods; a region with …
Ocular Ultrasonography And Echobiometry In Sheep, Hakim Athar, Jalal Udin Parrah, Nida Handoo, Abdul Qayoom Mir, Mehraj Udin Dar, Beenish Qureshi
Ocular Ultrasonography And Echobiometry In Sheep, Hakim Athar, Jalal Udin Parrah, Nida Handoo, Abdul Qayoom Mir, Mehraj Udin Dar, Beenish Qureshi
Turkish Journal of Veterinary & Animal Sciences
Ocular sonography and echobiometry was performed in local nondescript sheep (N = 36) of different age group and in adult corriedale (N = 60), crossbred Fec B (N = 48), and Poll Dorset cross breed sheep (N = 48). Cornea, anterior, posterior lens capsule, and retinal rim appeared hyperechoic whereas aqueous, vitreous humor, and nucleus of lens were anechoic. Significant increase (P < 0.05) in all the echobiometric measurement of the ocular distance except central corneal thickness and retinal rim thickness was recorded in the older animals (2 year old) compared to values recorded in animals at one month of age in local nondescript sheep. Significant differences (P < 0.05) in few recorded ocular biometric measurements were also noticed among different sheep breeds. However, the difference in the various ocular biometric measurements between the right and left eyes was nonsignificant. The results of this study provide the base line information regarding the ocular echobiometry in sheep that are relevant to comparative ocular anatomy and can be useful for ultrasonographic evaluation of different ocular affections in sheep.
Embryonic Mortality In Sheep: A Review, Pavitra Chundekkad, Barbara Blaszczyk, Tomasz Stankiewicz
Embryonic Mortality In Sheep: A Review, Pavitra Chundekkad, Barbara Blaszczyk, Tomasz Stankiewicz
Turkish Journal of Veterinary & Animal Sciences
Despite extensive research on the reproductive health of domestic animals, embryonic mortality continues to be a problem that brings about significant losses for both commercial breeding and scientific research. This review paper discusses the genetic and environmental factors that affect embryo mortality rates in sheep along with the different techniques developed to overcome this issue. Additionally, one of the objectives of this study is to emphasize the importance of the use of time-lapse cinematography and color Doppler ultrasonography as quick and reliable methods for early detection of pregnancy and embryonic death.
Prevalence Of Gastrointestinal Parasitic Infections In Cattle And Sheep In Two Regions Ofromania, Catalin Bogdan Sirbu, Kalman Imre, Gheorghe Darabus, Tiana Suici, Bogdan Mates, Sorin Morariu
Prevalence Of Gastrointestinal Parasitic Infections In Cattle And Sheep In Two Regions Ofromania, Catalin Bogdan Sirbu, Kalman Imre, Gheorghe Darabus, Tiana Suici, Bogdan Mates, Sorin Morariu
Turkish Journal of Veterinary & Animal Sciences
Amongst ruminant livestock diseases, the gastrointestinal (GIT) helminths and protozoan parasite infections result in a significant socio-economic concern worldwide. During the period between October 2017 and November 2018, a total of 788 fresh fecal specimens from cattle (n = 303) and sheep (n = 485) were screened for the presence of gastrointestinal helminths and protozoan parasites in two historical regions (Crisana and Banat) of western Romania. Laboratory processing of fecal samples with the simple salt flotation (Willis) and sedimentation techniques, followed by microscopic analysis revealed that 86.1% of cattle and 92.6% of sheep were found to be positive for at …
A Phylogenetic Analysis Of Mycoplasma Strains Circulating In Sheep Pneumonia In Thekars Region Of Turkey, Olcay Öztürkler, Sali̇h Otlu
A Phylogenetic Analysis Of Mycoplasma Strains Circulating In Sheep Pneumonia In Thekars Region Of Turkey, Olcay Öztürkler, Sali̇h Otlu
Turkish Journal of Veterinary & Animal Sciences
This study aimed to investigate Mycoplasma species by cultural and molecular methods in 250 pneumonics and 30 healthy appearance sheep lungs brought from slaughterhouse and butcheries in the Kars region of Turkey. The phylogenetic positioning of the bacterial strains to be obtained in the present study was another remarkable outcome of the study. Mycoplasma isolation was achieved solely in 26 (10.4%) of 250 sheep lungs with pneumonia. Mycoplasma ovipneumoniae was found predominant species in 12 (46.15%) of samples and that was followed by Mycoplasma arginini in 4 (15.38%) of strains. Moreover, two samples were found to be co-infected with the …
Effects Of Activated Charcoal And Zeolite On Serum Lipopolysaccharides And Someinflammatory Biomarkers Levels In Experimentally Induced Subacute Ruminal Acidosis Inlambs, Ali Abbas Nikvand, Mohammad Nouri, Mohammad Razi Jalali, Mohammad Reza Teimory Azadbakht
Effects Of Activated Charcoal And Zeolite On Serum Lipopolysaccharides And Someinflammatory Biomarkers Levels In Experimentally Induced Subacute Ruminal Acidosis Inlambs, Ali Abbas Nikvand, Mohammad Nouri, Mohammad Razi Jalali, Mohammad Reza Teimory Azadbakht
Turkish Journal of Veterinary & Animal Sciences
Bacterial lipopolysaccharides (LPS) play a key role in the pathogenesis of subacute ruminal acidosis (SARA). Limited attempts have been made to inhibit LPS absorption during SARA. Therefore, this study aimed to evaluate the impacts of oral usage of activated charcoal and zeolite on serum LPS and acute phase proteins (APPs) levels in lambs with SARA. Fifteen lambs were randomly assigned to 3 groups, each containing 5 lambs: control, activated charcoal, and zeolite. In all groups, SARA was induced for 7 days through increasing the crushed corn ration to 52.8% and reducing the rumen pH to the range of 5.7-5.6. In …
The Prevalence Of Gastrointestinal Nematodes In Sheep (Ovis Aries) In The Central Andsouth-Eastern Regions Of Ukraine, Vitalii Melnychuk, Valentyna Yevstafieva, Tetiana Bakhur, Anatoliy Antipov, Diana Feshchenko
The Prevalence Of Gastrointestinal Nematodes In Sheep (Ovis Aries) In The Central Andsouth-Eastern Regions Of Ukraine, Vitalii Melnychuk, Valentyna Yevstafieva, Tetiana Bakhur, Anatoliy Antipov, Diana Feshchenko
Turkish Journal of Veterinary & Animal Sciences
The article investigates into the species composition of agents causing gastrointestinal tract nematodes in sheep. It determines the forms of nematodes communities. For this purpose, 710 sheep were examined, 79.58% of which were infected. The fauna of nematodes was represented by 15 species of helminths from 12 genera: Bunostomum, Oesophagostomum, Nematodirus, Chabertia, Cooperia, Trichostrongylus, Ostertagia, Haemonchus, Trichuris, Aonchotheca, Strongyloides, and Skrjabinema. The indicators of infestation intensity in sheep ranged from 1 to 93 specimens per animal, and the abundance index ranged from 0.01 to 16.96 specimens per animal. The most common nematodes are H. contortus (prevalence, 61.97%), O. circumcincta (59.58%), …
Antimicrobial Resistance And Phenotypic And Molecular Detection Of Extended-Spectrum Ss-Lactamases Among Extraintestinal Escherichia Coli Isolated From Pneumonic And Septicemic Sheep And Goats In Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Jyoti Kumar, Shivendra Kumar Dixit, Rajendra Kumar Meena, Bhupendra Nath Tripathi
Antimicrobial Resistance And Phenotypic And Molecular Detection Of Extended-Spectrum Ss-Lactamases Among Extraintestinal Escherichia Coli Isolated From Pneumonic And Septicemic Sheep And Goats In Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Jyoti Kumar, Shivendra Kumar Dixit, Rajendra Kumar Meena, Bhupendra Nath Tripathi
Turkish Journal of Veterinary & Animal Sciences
The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep and goats and to determine antimicrobial resistance (AMR) patterns of the isolates and evaluate them for the presence of extended-spectrum ß-lactamase (ESBL) using the phenotypic and molecular methods. Pneumonic lung tissues and heart blood samples were collected by performing necropsy on sheep (n = 96) and goats (n = 08). The samples were processed and used for bacterial isolation. E. coli isolates (n = 58) including 53 from 34 sheep and 5 from 3 goats were recovered and identified by the cultural and biochemical characteristics and …
Management Of A Coxiella Burnetii-Infected Sheep Flock After An Outbreak Of Q Fever In Humans, Jana Avbersek, Mateja Pate, Andrej Skibin, Matjaz Ocepek, Brane Krt
Management Of A Coxiella Burnetii-Infected Sheep Flock After An Outbreak Of Q Fever In Humans, Jana Avbersek, Mateja Pate, Andrej Skibin, Matjaz Ocepek, Brane Krt
Turkish Journal of Veterinary & Animal Sciences
Following an outbreak of Q fever in a group of students who contracted the infection during a training course on a sheep farm, a detailed investigation of the sheep flock involved was conducted. Of 478 flock animals, 60 Coxiella burnetii ELISA-positive and 60 ELISA-negative ewes were selected for the trial and divided into four groups. A month after the initial ELISA screening, all ewes in the flock (except the control group) were vaccinated. Sequentially collected blood samples were tested with ELISA and PCR; feces, milk, manure, bedding, and soil were tested with PCR. The immune response to the vaccination was …
Molecular Detection Of Virulent Mannheimia Haemolytica And Pasteurella Multocida In Lung Tissues Of Pneumonic Sheep From Semiarid Tropics, Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Rajendra Kumar Meena
Molecular Detection Of Virulent Mannheimia Haemolytica And Pasteurella Multocida In Lung Tissues Of Pneumonic Sheep From Semiarid Tropics, Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Rajendra Kumar Meena
Turkish Journal of Veterinary & Animal Sciences
The present study was planned to detect the genetic elements of Mannheimia haemolytica and Pasteurella multocida in pneumonic sheep lungs. Pneumonia was diagnosed on the basis of gross pathological lesions. Lung tissues were collected at necropsy of sheep (n = 96) and subjected to isolation of total DNA. The M. haemolytica-specific PHSSA and Rpt2 genes, and the P. multocidaspecific KMT1 and the Omp87 genes were amplified using polymerase chain reaction (PCR). A housekeeping gene targeting the sheep cellular mitochondrial 12S ribosomal DNA was used as an internal control. PCR reactions were optimized using the positive and negative controls. Gene-specific PCR …
Evaluation Of Biochemical Parameters And Oxidative Stress In Sheep Naturally Infected With Dicrocoelium Dendriticum And Hydatid Cysts, Mi̇yase Çinar, Meral Aydeni̇zöz, Sami̇ Gökpinar, Güzi̇n Çamkerten
Evaluation Of Biochemical Parameters And Oxidative Stress In Sheep Naturally Infected With Dicrocoelium Dendriticum And Hydatid Cysts, Mi̇yase Çinar, Meral Aydeni̇zöz, Sami̇ Gökpinar, Güzi̇n Çamkerten
Turkish Journal of Veterinary & Animal Sciences
The purpose of the study was to investigate the lipid peroxidation and antioxidant status as well as biochemical profile in sheep naturally infected with Dicrocoelium dendriticum and hydatid cysts. Ten sheep naturally infected with D. dendriticum, 10 sheep naturally infected with hydatid cysts, and 10 healthy sheep were used. The blood and visceral organs of sheep brought to a slaughterhouse in the region of Kırıkkale were checked for D. dendriticum and hydatid cysts. Plasma total protein, albumin, total bilirubin, total cholesterol, Ca, Mg, inorganic P, Cu, Zn, vitamin A, vitamin C, vitamin E, ß-carotene, AST, ALT, GGT, ALP, erythrocyte MDA …
Comparison Of Pre- And Postpartum Serum Leptin, Ghrelin, And Lipid Levels In Sheep, Ethem Mutlu Temi̇zel, Hüseyi̇n Ci̇han, Pinar Levent, Ahmet Saril, Yeşi̇m Özarda, Zeki̇ Yilmaz
Comparison Of Pre- And Postpartum Serum Leptin, Ghrelin, And Lipid Levels In Sheep, Ethem Mutlu Temi̇zel, Hüseyi̇n Ci̇han, Pinar Levent, Ahmet Saril, Yeşi̇m Özarda, Zeki̇ Yilmaz
Turkish Journal of Veterinary & Animal Sciences
Serum leptin and ghrelin play important roles in energy metabolism and inducing appetite during pregnancy and lactation. We investigated the serum levels of leptin and ghrelin and their relationship with the duration of lactation and serum biochemical responses during the prepartum (last week before lambing) and postpartum periods in sheep. They were also evaluated between pregnant and nonpregnant (control ewes) ewes. For these purposes, pregnant (n = 15) and control Awassi ewes (n = 15) that were 2 years of age were used in this study. Blood samples were collected 1 week before the expected date of lambing (baseline) and …
Comparison Of Different Paternity Test Panels In Sheep, Onur Yilmaz, İbrahi̇m Cemal, Behi̇ç Coşkun, Yusuf Zi̇ya Oğrak, Nezi̇h Ata, Orhan Karaca
Comparison Of Different Paternity Test Panels In Sheep, Onur Yilmaz, İbrahi̇m Cemal, Behi̇ç Coşkun, Yusuf Zi̇ya Oğrak, Nezi̇h Ata, Orhan Karaca
Turkish Journal of Veterinary & Animal Sciences
The aims of this study were to evaluate different paternity test panels with 17 microsatellite markers for their effectiveness in paternity analysis and to identify pedigree error rates in the Kangal Akkaraman breed of sheep. The animal material for the study consisted of 175 Kangal Akkaraman sheep, 35 rams, and their 140 offspring, raised on farms and bred using controlled mating. The panels were created according to probability of exclusion (PE) of 17 studied microsatellites. A total of 240 alleles were detected across 17 microsatellite loci. The overall mean value of the polymorphic information content (PIC) (0.78) indicated that the …
Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China, Yongshuai Peng, Meng Qi, Fuchun Jian, Jinhong Wang, Yali Lv, Jiujian Wei, Rongjun Wang, Longxian Zhang, Changshen Ning
Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China, Yongshuai Peng, Meng Qi, Fuchun Jian, Jinhong Wang, Yali Lv, Jiujian Wei, Rongjun Wang, Longxian Zhang, Changshen Ning
Turkish Journal of Veterinary & Animal Sciences
Tick-borne diseases are one of the most important classes of disease in animal husbandry and cause severe economic losses. In this study, 660 adult female ticks were collected from sheep in nine localities in Henan Province, China. All were identified as Haemaphysalis longicornis, confirmed with light microscopy and PCR amplification. The pathogens identified in these ticks included bacteria of the genus Anaplasma (Anaplasma ovis, A. bovis, A. phagocytophilum) and piroplasmal protozoans (Theileria luwenshuni, Babesia motasi). Our results show the high prevalence of A. bovis (20.4%, 135/660) and T. luwenshuni (14.4%, 95/660) in the ticks from sheep, whereas A. ovis, A. …
Co-Existence Of Bovine Viral Diarrhea And Border Disease Viruses In A Sheep Flock Suffering From Abortus And Diarrhea, Peli̇n Tuncer Göktuna, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ
Co-Existence Of Bovine Viral Diarrhea And Border Disease Viruses In A Sheep Flock Suffering From Abortus And Diarrhea, Peli̇n Tuncer Göktuna, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ
Turkish Journal of Veterinary & Animal Sciences
Pestivirus infections have a huge economic impact on livestock production. In 2014, an aborted fetus from a sheep flock suffering from abortus and diarrhea was submitted for virological diagnosis. Due to the positive result of the sample for pestivirus and continuing clinical symptoms in the flock, all of the animals were sampled individually. Blood samples for serum and peripheral blood mononuclear cells were collected from 93 animals (5 rams, 26 lambs, and 62 sheep). During sampling 1 ocular and 4 rectal swab samples were obtained from lambs that had a clinical eye problem and diarrhea, respectively. Additionally 5 aborted fetuses …
The Effect Of Combining Vitamin E And C On The Viability Improvement Of Transfected Ovine Spermatogonial Stem Cells After Cryopreservation And Thawing, Hosein Shabani, Mohammad Zandi, Hamideh Ofogi, Mohammad Reza Sanjabi, Khosro Hoseini Pajooh
The Effect Of Combining Vitamin E And C On The Viability Improvement Of Transfected Ovine Spermatogonial Stem Cells After Cryopreservation And Thawing, Hosein Shabani, Mohammad Zandi, Hamideh Ofogi, Mohammad Reza Sanjabi, Khosro Hoseini Pajooh
Turkish Journal of Veterinary & Animal Sciences
The present study aimed to identify whether vitamin E and C and their combination in basal freezing medium improved the viability of postthaw transfected spermatogonial stem cells (SSCs). For this purpose, SSCs were harvested by enzymatic digestion twice. After enrichment and culture on Sertoli cell feeder layer, SSCs were characterized by using alkaline phosphatase staining and expression of oct-4 and c-kit genes as specific stem cell markers. As to the transfection of SSCs, different concentrations of DNA (0.2, 0.4, and 0.8 μg) and turbofect (0.4 and 0.8 μL) in 100 μL of medium were studied. The results showed that cryopreservation …
Comparative Evaluations Of Tear Secretion In Healthy And Infectious Keratoconjunctivitis Romanov Sheep With Schirmer Tear Test And Phenol Red Thread Tear Test, Mustafa Bariş Akgül, Ni̇hat Şindak, Ali̇ Gülaydin, Doğukan Özen, Serpi̇l Kahya Demi̇rbi̇lek, Kadi̇r Sulu
Comparative Evaluations Of Tear Secretion In Healthy And Infectious Keratoconjunctivitis Romanov Sheep With Schirmer Tear Test And Phenol Red Thread Tear Test, Mustafa Bariş Akgül, Ni̇hat Şindak, Ali̇ Gülaydin, Doğukan Özen, Serpi̇l Kahya Demi̇rbi̇lek, Kadi̇r Sulu
Turkish Journal of Veterinary & Animal Sciences
The aim of this study is to determine the amount of tear secretion in sheep with infectious keratoconjunctivitis and healthy ones in the Siirt region by Schirmer tear test and phenol red thread tear test. A total of 47 Romanov sheep, consisting of sheep with infectious keratoconjunctivitis (n = 6) and healthy sheep (n = 41), constituted the study material. Phenol red thread tear test followed by Schirmer tear test was performed on both eyes of the animals, properly taken at a control visit on their feet. The Schirmer tear test and phenol red thread tear test values were measured …
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Turkish Journal of Veterinary & Animal Sciences
Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …
Seroprevalence Of Caseous Lymphadenitis In Kangal Akkaraman Sheep, Öznur Aslan, Kadi̇r Semi̇h Gümüşsoy, İlknur Karaca Bekdi̇k, Aytaç Akçay, Ömer Orkun Demi̇ral
Seroprevalence Of Caseous Lymphadenitis In Kangal Akkaraman Sheep, Öznur Aslan, Kadi̇r Semi̇h Gümüşsoy, İlknur Karaca Bekdi̇k, Aytaç Akçay, Ömer Orkun Demi̇ral
Turkish Journal of Veterinary & Animal Sciences
In this study, the seroprevalence of Corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis (CLA) infection, in Kangal Akkaraman sheep flocks was examined. For this purpose, blood samples were collected from 25 different sheep flocks (n = 379). According to the ELISA results, the numbers of CLA-positive and CLA-negative samples were 138 (36.4%) and 241 (63.6%), respectively. CLA positivity was observed in 35.7% of the females (n = 241), 37.7% of the males (n = 138), 40.2% of the sheep with clinical symptoms (n = 184), and 32.8% of the sheep with no clinical symptoms (n = 195) (P > 0.05). …
Cast/Mspi Gene Polymorphism And Its Impact On Growth Traits Of Soviet Merinoand Salsk Sheep Breeds In The South European Part Of Russia, Ivan Fedorovich Gorlov, Nadezhda Vasilevna Shirokova, Alexander Vasilevich Randelin, Valeriya Nikolaevna Voronkova, Natalya Ivanovna Mosolova, Elena Yurevna Zlobina, Yury Anatolevich Kolosov, Nekruz Farkhodovich Bakoev, Mariya Anatolevna Leonova, Sirozhdin Yusufovich Bakoev, Anatoly Yurevich Kolosov, Lubov Vladimirovna Getmantseva
Cast/Mspi Gene Polymorphism And Its Impact On Growth Traits Of Soviet Merinoand Salsk Sheep Breeds In The South European Part Of Russia, Ivan Fedorovich Gorlov, Nadezhda Vasilevna Shirokova, Alexander Vasilevich Randelin, Valeriya Nikolaevna Voronkova, Natalya Ivanovna Mosolova, Elena Yurevna Zlobina, Yury Anatolevich Kolosov, Nekruz Farkhodovich Bakoev, Mariya Anatolevna Leonova, Sirozhdin Yusufovich Bakoev, Anatoly Yurevich Kolosov, Lubov Vladimirovna Getmantseva
Turkish Journal of Veterinary & Animal Sciences
The purpose of this work was to study MspI polymorphism in the CAST gene in Soviet Merino and Salsk sheep breeds in the South European part of Russia and to find its relation with growth traits. Soviet Merino sheep have been found to have the M and N alleles with a frequency of 0.88 and 0.12 and the MM, MN, and NN genotypes with a frequency of 0.82, 0.12, and 0.06, respectively. Salsk sheep have been established to have the M and N alleles with a frequency of 0.89 and 0.11 and two genotypes, MM and MN, with a frequency …
Comparison Of Pcr Tests For The Detection Of Mycoplasma Agalactiae In Sheep And Goats, Hüban Göçmen, Ruben Sebastian Rosales, Roger David Ayling, Mi̇hri̇ban Ülgen
Comparison Of Pcr Tests For The Detection Of Mycoplasma Agalactiae In Sheep And Goats, Hüban Göçmen, Ruben Sebastian Rosales, Roger David Ayling, Mi̇hri̇ban Ülgen
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to compare PCR tests for the detection of Mycoplasma agalactiae in sheep and goats. Samples obtained from sheep and goats in Turkey were tested to determine the presence of M. agalactiae. Mycoplasma culture and two direct PCR methods based on the uvrC gene and polC gene were compared with PCR of the 16S rRNA gene followed by denaturing gradient gel electrophoresis (PCR/DGGE) on DNA extracted from clinical samples (following culture enrichment). A total of 234 samples were examined: 9.4% and 13.2% were positive for M. agalactiae based on the uvrC gene and polC gene …
Evaluation Of Tear And Serum Trace Elements (Copper, Selenium, And Cobalt) In Sheep, Duygu Tarhan, Si̇nem Ülgen, Fatma Ateş Alkan, Di̇lek Olgun Erdi̇kmen, Çağla Parkan Yaramiş, Mehmet Erman Or, Ümi̇t Bora Barutçu
Evaluation Of Tear And Serum Trace Elements (Copper, Selenium, And Cobalt) In Sheep, Duygu Tarhan, Si̇nem Ülgen, Fatma Ateş Alkan, Di̇lek Olgun Erdi̇kmen, Çağla Parkan Yaramiş, Mehmet Erman Or, Ümi̇t Bora Barutçu
Turkish Journal of Veterinary & Animal Sciences
Metabolic diseases such as enzootic ataxia, white muscle disease, and white liver disease are among the most important problems of livestock for sheep. The aim of this study was to analyze whether tears can be used as an alternative diagnostic method in determination of diseases that result from lack or excess of copper, selenium, and cobalt. Samples of serum and tears taken from 45 healthy sheep of the Kivircik breed aged between 2 and 4 years were analyzed. The concentrations of copper, selenium, and cobalt were measured in serum and tears as 118.0 ± 36.0, 58.0 ± 15.0, and 0.5 …
Polymorphism Of Exon 2 Of Dio2 Gene And Its Association Withseasonal Reproduction In Sheep, Jianning He, Dongwei Huang, Ran Di, Jinxin Wang, Mingxing Chu, Qiuyue Liu, Wenping Hu, Xiangyu Wang, Zhangyuan Pan
Polymorphism Of Exon 2 Of Dio2 Gene And Its Association Withseasonal Reproduction In Sheep, Jianning He, Dongwei Huang, Ran Di, Jinxin Wang, Mingxing Chu, Qiuyue Liu, Wenping Hu, Xiangyu Wang, Zhangyuan Pan
Turkish Journal of Veterinary & Animal Sciences
DIO2 was studied as a candidate gene for reproduction in sheep. Three pairs of primers were designed to detect single nucleotide polymorphisms (SNPs) of exon 2 of DIO2 in nonseasonal and seasonal reproduction breeds. The polymorphisms were detected using SeqMan II and analyzed by PCR-RFLP. The sheep DIO2 gene was cloned and its mutations were detected in five sheep breeds whose reproductive seasonality and litter sizes were different. Association analyses were performed to reveal the relationships between SNPs and reproductive seasonality as well as litter size in sheep. The results showed that a 1041-bp DNA fragment of the sheep DIO2 …
Presence Of Carbohydrate-Digesting Enzymes Throughout The Digestive Tract Of Sheep, Renata Miltko, Grzegorz Belzecki, Barbara Kowalik, Jacek Skomial
Presence Of Carbohydrate-Digesting Enzymes Throughout The Digestive Tract Of Sheep, Renata Miltko, Grzegorz Belzecki, Barbara Kowalik, Jacek Skomial
Turkish Journal of Veterinary & Animal Sciences
The activity of enzymes that digest carbohydrates at various points in the gastrointestinal tract of sheep was examined. In addition, weight and contents of digestive tract segments were examined. The experiment was performed on six adult ewes fed an 85% hay diet. The morphometric measurements revealed that the alimentary tract constituted 32% of the sheep's body mass and wet rumen contents constituted 70% of wet mass of the total digestive tract content. The protozoal population in the rumen varied from 37.2 × 104 to 54.0 × 104/mL rumen fluid. In the examined samples, ciliates were identified as belonging to the …
Ovine Coccidiosis: Prevalence And Associated Risk Factors Inand Around Addis-Zemen, Northwest Ethiopia, Abinet Lakew, Zewdu Seyoum
Ovine Coccidiosis: Prevalence And Associated Risk Factors Inand Around Addis-Zemen, Northwest Ethiopia, Abinet Lakew, Zewdu Seyoum
Turkish Journal of Veterinary & Animal Sciences
This cross-sectional study was conducted in and around Addis-Zemen from November 2014 to April 2015 to determine the prevalence of ovine coccidiosis and to identify associated risk factors. Faecal samples were collected directly from the rectum of 384 randomly selected animals. A centrifugal faecal flotation technique using saturated salt solution was used to detect Eimeria oocysts. Out of 384 samples, only 88 were found to contain Eimeria oocysts. Eimeria infection was recorded in all age and sex groups of the study animals. A strong significant association was observed (P < 0.05) between Eimeria infection and the faecal consistency, production system, hygienic status, age, and body condition of the sampled animals. However, an insignificant (P > 0.05) association was recorded between Eimeria infection and the origins and …