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Full-Text Articles in Veterinary Medicine
Single Nucleotide Polymorphism Identification And Characterization Of Gnrhr Gene In Bos Taurus And Bos Indicus Cattle, Ramesha Kerekoppa, Marappa Basavaraju, Akhila Rao, Gujjar Ramachandra Geetha, Kataktalware Mukund, Jeyakumar Sakthivel
Single Nucleotide Polymorphism Identification And Characterization Of Gnrhr Gene In Bos Taurus And Bos Indicus Cattle, Ramesha Kerekoppa, Marappa Basavaraju, Akhila Rao, Gujjar Ramachandra Geetha, Kataktalware Mukund, Jeyakumar Sakthivel
Turkish Journal of Veterinary & Animal Sciences
The hypothalamic gonadotropin-releasing hormone receptor (GnRHR) plays an essential physiological role in reproductive function. In this investigation, we studied genetic variation in the entire coding region of GnRHR gene using PCR-SSCP technique in 250 Bos taurus (Holstein Friesian, Jersey) and Bos indicus (Malnad Gidda, Deoni) bulls. The genetic variants in GnRHR gene were determined by PCR-SSCP technique by using 5 sets of primers. SSCP analysis of fragment 1, comprising exon 1, and fragment 3, comprising exon 2, revealed 2 SSCP patterns in all breeds under study, while fragment 2 of exon 1 and fragment 4, comprising exon 3, revealed 3 …
Estimating Allele Frequencies Of Some Hereditary Diseases In Holstein Cattlereared In Burdur Province, Turkey, Özgecan Korkmaz Ağaoğlu, Ali̇ Reha Ağaoğlu, Mustafa Saatci
Estimating Allele Frequencies Of Some Hereditary Diseases In Holstein Cattlereared In Burdur Province, Turkey, Özgecan Korkmaz Ağaoğlu, Ali̇ Reha Ağaoğlu, Mustafa Saatci
Turkish Journal of Veterinary & Animal Sciences
The economics of dairy cattle breeding around the world has been significantly affected by the autosomal recessive hereditary diseases of bovine leukocyte adhesion deficiency (BLAD), factor XI deficiency (FXID), bovine citrullinemia (BC), and deficiency of uridine monophosphate synthase (DUMPS). The aim of this study was to determine BLAD, FXID, BC, and DUMPS alleles in Holstein cattle raised in Burdur Province in Turkey. This study involved taking blood samples randomly from 500 head of Holstein cattle at various dairy farms in different towns in Burdur Province. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis was employed to identify cows …
Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle, Kadi̇r Semi̇h Gümüşsoy, Tuba İça, Seçi̇l Abay, Fuat Aydin, Harun Hizlisoy
Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle, Kadi̇r Semi̇h Gümüşsoy, Tuba İça, Seçi̇l Abay, Fuat Aydin, Harun Hizlisoy
Turkish Journal of Veterinary & Animal Sciences
In this study, the presence of paratuberculosis was investigated by serological and molecular methods in herds of dairy cattle. Blood, milk, and stool samples of 147 cows aged 2 years old or older with chronic diarrhea were collected. A California mastitis test (CMT) was performed on milk samples. Indirect paratuberculosis enzyme-linked immunosorbent assay (ELISA) test was used for serological investigation. Polymerase chain reaction (PCR) was utilized for molecular identification of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and stool samples. Immunomagnetic separation (IMS) method was used for milk and fecal samples. Eighteen (12.24%), 44 (29.93%), 36 (24.49%), 28 (19.05%), and …