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Turkish Journal of Veterinary & Animal Sciences

2018

ELISA

Articles 1 - 3 of 3

Full-Text Articles in Veterinary Medicine

Evaluation Of Real-Time Pcr, Mat, And Recombinant Lipl32-Based Elisa For The Diagnosis Of Canine Leptospirosis In A Disease-Endemic South Indian State, Kerala, Siju Joseph, Mangattumuruppel Mini, Vamshi Krishna Sriram, Ramachandran Ambily, Thazhathuveettil Aravindakshan, Sreedharannair Ajithkumar Jan 2018

Evaluation Of Real-Time Pcr, Mat, And Recombinant Lipl32-Based Elisa For The Diagnosis Of Canine Leptospirosis In A Disease-Endemic South Indian State, Kerala, Siju Joseph, Mangattumuruppel Mini, Vamshi Krishna Sriram, Ramachandran Ambily, Thazhathuveettil Aravindakshan, Sreedharannair Ajithkumar

Turkish Journal of Veterinary & Animal Sciences

Canine samples (n = 425) collected from cases of suspected leptospirosis over a period of 6 years were tested using the microscopic agglutination test (MAT), recombinant LipL32-based IgM and IgG ELISA, and real-time PCR (RT PCR). A prevalence of 69.41% was recorded by MAT. Among the different tests, IgG ELISA was observed to have maximum agreement with that of MAT (k-0.810), followed by IgM ELISA (k-0.762.). RT PCR had only slight agreement with MAT (k-0.156). However, the percentage positivity of RT PCR with samples with MAT titers of 1:100 and 1:200 were 76.47% and 85.96%, respectively, indicating the reliability of …


Epidemiological Survey And Economic Significance Of Bovine Hypodermosison The Kars Plateau In The Northeast Anatolia Region Of Turkey, Gencay Taşkin Taşçi, Bariş Sari, Ni̇lgün Parmaksizoğlu Aydin, Zati̇ Vatansever, Nesli̇han Ölmez, Ati̇la Akça, Mükremi̇n Özkan Arslan Jan 2018

Epidemiological Survey And Economic Significance Of Bovine Hypodermosison The Kars Plateau In The Northeast Anatolia Region Of Turkey, Gencay Taşkin Taşçi, Bariş Sari, Ni̇lgün Parmaksizoğlu Aydin, Zati̇ Vatansever, Nesli̇han Ölmez, Ati̇la Akça, Mükremi̇n Özkan Arslan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the prevalence and seroprevalence of hypodermosis and to calculate the economic loss associated with hide damage in the cattle on the Kars Plateau, Turkey. A total of 6563 randomly selected cattle were examined by skin palpation from 1 April to 31 May 2014. A subset of 465 serum samples was collected from October to November in 2014 and analyzed using a commercial ELISA kit. The overall prevalence of hypodermosis was 16.9% in the cattle examined by skin palpation. In respect of each of the risk factors assessed, the highest prevalence rates were …


Scenario Of Porcine Cysticercosis And Human Taeniasis In Maharashtra State, India, Vilas Vaidya, Ashish Paturkar, Ravindra Zende, Mukulesh Gatne, Dhananjay Dighe, Rupesh Waghmare, Shilpa Laxmanrao Moon, Sujata Bhave, Priyanka Jadhav, Nilam Wavhal Jan 2018

Scenario Of Porcine Cysticercosis And Human Taeniasis In Maharashtra State, India, Vilas Vaidya, Ashish Paturkar, Ravindra Zende, Mukulesh Gatne, Dhananjay Dighe, Rupesh Waghmare, Shilpa Laxmanrao Moon, Sujata Bhave, Priyanka Jadhav, Nilam Wavhal

Turkish Journal of Veterinary & Animal Sciences

Cysticercosis/taeniasis is one of the neglected parasitic zoonoses in developing countries like India. A study was conducted for a period of 7 years from 2010 to 2017 during which 13,596 pig and 1238 human samples were screened to learn the exact scenario of cysticercosis/taeniasis in the different regions of Maharashtra State. Prevalence of cysticercosis/taeniasis in pigs and humans was recorded using various diagnostic methods such as meat inspection and serological methods involving ELISA. The overall prevalence of cysticercosis in pigs was found to be 0.88% by postmortem examination and 0.9% by PCR assay, whereas prevalence of taeniasis in humans was …