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Full-Text Articles in Veterinary Medicine
Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle, Kadi̇r Semi̇h Gümüşsoy, Tuba İça, Seçi̇l Abay, Fuat Aydin, Harun Hizlisoy
Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle, Kadi̇r Semi̇h Gümüşsoy, Tuba İça, Seçi̇l Abay, Fuat Aydin, Harun Hizlisoy
Turkish Journal of Veterinary & Animal Sciences
In this study, the presence of paratuberculosis was investigated by serological and molecular methods in herds of dairy cattle. Blood, milk, and stool samples of 147 cows aged 2 years old or older with chronic diarrhea were collected. A California mastitis test (CMT) was performed on milk samples. Indirect paratuberculosis enzyme-linked immunosorbent assay (ELISA) test was used for serological investigation. Polymerase chain reaction (PCR) was utilized for molecular identification of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and stool samples. Immunomagnetic separation (IMS) method was used for milk and fecal samples. Eighteen (12.24%), 44 (29.93%), 36 (24.49%), 28 (19.05%), and …
Development And Validation Of Sandwich Quantitative Elisa Prototypebased On The Bovine Ifng For The Detection Of Cellular Immunity, Oktay Genç, Özlem Büyüktanir, Gülnur Serdar, Yunus Kiliçoğlu, Sali̇h Otlu
Development And Validation Of Sandwich Quantitative Elisa Prototypebased On The Bovine Ifng For The Detection Of Cellular Immunity, Oktay Genç, Özlem Büyüktanir, Gülnur Serdar, Yunus Kiliçoğlu, Sali̇h Otlu
Turkish Journal of Veterinary & Animal Sciences
Immunodiagnostic tests based on in vitro enzymatic methods are mostly preferred to in vivo methods for the detection of cellular immunity due to their cost, applicability, repeatability, and higher sensitivity. Therefore, the development of a quantitative sandwich ELISA prototype detecting bovine IFNg (bIFNg) was planned. In this study, two clone originated monoclonal antibodies were used for capturing the plates, and biotin conjugated monoclonal Ab was used as a secondary antibody in sandwich ELISA. Different approaches such as inter- and intraassay precision and dilution parallelism were evaluated for test validation. The correlation of coefficient variation that is over 95% and 5% …