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Full-Text Articles in Veterinary Medicine
Changes In Carbonic Anhydrase Activity And Gene Expression Of Hsp70 In Rainbow Trout (Oncorhynchus Mykiss) Muscle After Exposure To Some Metals, Hakan Söyüt, Şükrü Beydemi̇r, Saltuk Buğrahan Ceyhun, Orhan Erdoğan, Eli̇f Duygu Kaya
Changes In Carbonic Anhydrase Activity And Gene Expression Of Hsp70 In Rainbow Trout (Oncorhynchus Mykiss) Muscle After Exposure To Some Metals, Hakan Söyüt, Şükrü Beydemi̇r, Saltuk Buğrahan Ceyhun, Orhan Erdoğan, Eli̇f Duygu Kaya
Turkish Journal of Veterinary & Animal Sciences
The effects of some heavy metals Co(II), Cu(II), Zn(II), and Ag(I) on carbonic anhydrase (CA) activity from rainbow trout (RT) muscle were investigated. Moreover, the effects of these metals on the expression of heat shock protein 70 (Hsp70) gene in muscle tissue were examined by real-time quantitative PCR (RT-PCR) in muscle tissue after exposure to the metals at the end of 6, 12, 24, and 48 h. CA was purified with a specific activity of 2300 EU mg^{-1}, a yield of 19%, and 1080-fold. The molecular weights (Mw) of subunit and native enzyme were approximately 30 and 31 kDa, respectively. …
The Effect Of Maternal Undernutrition On Muscle Development In The Ovine Fetus*, Berjan Demi̇rtaş, Mukaddes Özcan
The Effect Of Maternal Undernutrition On Muscle Development In The Ovine Fetus*, Berjan Demi̇rtaş, Mukaddes Özcan
Turkish Journal of Veterinary & Animal Sciences
The effect of maternal undernutrition on muscle development of near-term ovine fetuses was studied in 2 experiments. In each experiment, Welsh mountain ewes were randomly assigned to either control (C) or nutrient-restricted (R) groups. In the first experiment, mild maternal undernutrition (85% of daily nutritional requirement (DNR)), and in the second experiment, severe maternal undernutrition (50% of DNR), were imposed between 0 and 70 days of gestation (dg). Controls for both groups were maintained on 100% of their DNR throughout the experiment. In each experiment, ewes were killed at 126 ± 1 dg by an intravenous injection of pentobarbitone. The …