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Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China, Hui Lan Wu, Peng Hui Hu, Jin Zhang
Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China, Hui Lan Wu, Peng Hui Hu, Jin Zhang
The Thai Journal of Veterinary Medicine
Wenyonella philiplevinei is one of the important pathogens of duck coccidiasis. In the present study, a portion of the 18S rDNA was amplified from W. philiplevinei by Polymerase chain reaction (PCR), cloned and then sequenced. The sequences of 18S rDNA of all samples were 422 bp in size. A + T content of the 18S rDNA sequences was 58-59%. Sequence comparison revealed that the similarity in 18S rDNA sequences between Hunan isolates and W. philiplevinei available (Guangdong isolate) was more than 98%. Intra-specific sequence variations within each of the Hunan isolates were 0-1.7%. However, the inter-specific sequence differences between W. …