Veterinary Medicine Commons^™

Absorption And Utilization Of Choline And Vitamin B12 In Lactating Dairy Cows Using Different Delivery Methods, Virginia Maria Artegoitia Etcheverry

Doctoral Dissertations

Choline and vitamin B12 are essential nutrients for growth and performances of production animals. However, both nutrients are extensively degraded during digestion in the rumen. This thesis comprised three experiments. First, four cows equipped with a rumen cannula and catheters in the portal vein and a mesenteric artery received a post-ruminal bolus of: 1) cyanocobalamin (CN-CBL) alone (0.1 g) [gram], 2) CN-CBL (0.1 g) + casein (10 g) or 3) CN-CBL (0.1 g) + whey proteins (10 g). After the bolus, blood samples were taken until 24 h [hour] post-bolus. The intestinal absorption of CN-CBL was greater when the vitamin …

Effect Of Small Interfering Rnas On In Vitro Replication And Gene Expression Of Feline Coronavirus, Eman Ahmed Mohamed Anis

Doctoral Dissertations

Feline corononavirus (FCoV) infection is ubiquitous in domestic cat populations worldwide and is usually associated with subclinical or mild enteritis. However, in some cats infection may result in the development of a fatal progressive disease called feline infectious peritonitis (FIP). FIP is considered to be the major cause of infectious-related death in pet cats. Currently, there is no protective vaccine or curative treatment to this highly fatal disease. In this study, we evaluated the ability of small interfering RNAs (siRNAs) to inhibit the in vitro viral replication and gene expression of FCoV as a potential treatment for FIP.

Five synthetic …

Effect Of A Leucine/Pyridoxine Nutraceutical On Energy Metabolism And Satiety In Lean And Obese Dogs, Maryanne Murphy

Doctoral Dissertations

Obesity is the most common nutritional disorder of pet dogs (prevalence of 23–59%). This 29-week study investigated a nutraceutical containing 1 g leucine and 13 mg pyridoxine designed to maintain lean muscle mass while decreasing adiposity when compared with positive and negative controls. After determining individual maintenance calorie requirements over 3 – 4 weeks, thirty-six healthy adult Beagles were divided into obesification, weight gain prevention, or maintain ideal body weight arms.

Obesity was induced and during a weight loss phase, excess calories were removed, and obesification dogs were given nutraceutical with CAD (ObN), placebo with CAD (ObP), or a therapeutic …

Cd4 Silencing In Thymocytes Is Opposed By The Enforced Association Of P300 Hat, Hdac1 Or Suv39h1 With Runx Transcription Factors, Christyne Ayne Kane

Doctoral Dissertations

The transcription factors Runx1 and Runx3 are required for permanent silencing of CD4 in maturing CD8+ thymocytes. Runx binding to consensus sites within the CD4 silencer region is required for CD4 silencing post-positive selection. The Runx nuclear matrix targeting sequence (NMTS) is required for CD4 silencing and is implicated in binding to the histone acetyltransferase (HAT) p300, histone deacetylases (HDAC), and histone methyltransferase (HMT) SUV39H1 proteins. Epigenetic modifications of chromatin or post-translational modifications of Runx itself as a result of Runx association with these enzymes may be important for establishment of long-term CD4 silencing. In this study, we show that …