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Full-Text Articles in Veterinary Medicine
Expression Of The Gprc5d Gene In Liaoning Cashmere Goats, Mei Jin, Xiuna Li, Jun Piao, Yangle Qu, Yimeng Wang, Jingai Piao, Mingxing Chu
Expression Of The Gprc5d Gene In Liaoning Cashmere Goats, Mei Jin, Xiuna Li, Jun Piao, Yangle Qu, Yimeng Wang, Jingai Piao, Mingxing Chu
Turkish Journal of Veterinary & Animal Sciences
This study aimed to characterize the expression the GPRC5D gene in the skin of the Liaoning Cashmere goat. Initially, the GPRC5D gene sequence was subjected to bioinformatics analysis. Second, semiquantitative RT-PCR was used to detect GPRC5D gene expression in the tissues. Third, in situ hybridization was used to locate GPRC5D gene expression in the primary and secondary hair follicles. Functional site analysis showed that GPRC5D has 7 transmembrane domains. RT-PCR showed that GPRC5D was expressed only in the skin. In situ hybridization showed that GPRC5D was expressed in the inner root sheath only. We speculated that GPRC5D may regulate cashmere …
Immunohistochemical Localization Of Glutathione Peroxidase 1 Enzyme And Its Gene Expression By Rt-Pcr In The Liver Tissue Of Healthy And Diabetic Mice, Turgay Deprem, Nurhayat Gülmez
Immunohistochemical Localization Of Glutathione Peroxidase 1 Enzyme And Its Gene Expression By Rt-Pcr In The Liver Tissue Of Healthy And Diabetic Mice, Turgay Deprem, Nurhayat Gülmez
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study is to examine the gene expression level of glutathione peroxidase 1 (GPx1) in the liver of healthy and diabetic mice, its localization in the tissue, and structural changes in the liver. In this study, 36 Swiss albino mice were divided into 3 groups: experimental (diabetic) (n = 15), sham (n = 15), and control (n = 6). Streptozotocin (100 mg/kg) was intraperitoneally administered to mice in the experimental group. While the gene expression level of GPx1 was determined using the RT-PCR method, its localization in the liver was determined using an immunohistochemical method. The GPx1 …
Immunohistochemical Distribution Of Glutathione Peroxidase And Its Gene Expression Via Rt-Pcr In The Liver Tissue Of Melatonin-Administered Mice, Serap Koral Taşçi, Nurhayat Gülmez
Immunohistochemical Distribution Of Glutathione Peroxidase And Its Gene Expression Via Rt-Pcr In The Liver Tissue Of Melatonin-Administered Mice, Serap Koral Taşçi, Nurhayat Gülmez
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study is to investigate the effect of exogenous administration of melatonin, which is a strong antioxidant agent, on the gene expression of glutathione peroxidase, which is one of the antioxidant enzymes, in the livers of mice and also to examine the immunohistochemical localization of the enzyme. The 24 male Swiss albino mice used in the study were divided into 3 groups: experimental (n = 8), sham (n = 8), and control (n = 8). A 10 mg/kg dose of melatonin was administered intraperitoneally to the experimental group for 4 weeks. Only ethanol and a serum physiological …
Pathotypic Characterization Of The Newcastle Disease Virus Isolated From Commercial Poultry In Northwest Iran, Elham Ahmadi, Seyed Ali Pourbakhsh, Malahat Ahmadi, Alireza Talebi
Pathotypic Characterization Of The Newcastle Disease Virus Isolated From Commercial Poultry In Northwest Iran, Elham Ahmadi, Seyed Ali Pourbakhsh, Malahat Ahmadi, Alireza Talebi
Turkish Journal of Veterinary & Animal Sciences
Despite vaccination, outbreaks of Newcastle disease in commercial poultry flocks in northwest Iran have been regularly reported in recent years. This research was proposed in order to characterize, through a molecular approach, the genetic nature of strains of Newcastle disease virus isolated from commercial poultry flocks in northwest Iran. Ten Newcastle disease virus isolates were obtained from various districts of northwest Iran in 2010. The isolates were screened with F gene-targeting reverse transcription polymerase chain reaction (RT-PCR). The amplified products of virulent strains were sequenced. The deduced amino acid sequence of the fusion protein cleavage site indicated the presence of …