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Full-Text Articles in Veterinary Medicine
The Effects Of Chemical Reagents And Physical Environment On The In Vitro And In Vivo Properties Of Adipose-Derived Multipotent Stromal Cells Isolated From Different Species, Wei Duan
LSU Doctoral Dissertations
Adult adipose derived mesenchymal stromal cells (ASCs) have been characterized in various species. Many factors may affect ASC fate and ASCs from different species may have different response to these factors. The first study was to identify the differences of the canine ASCs isolated from subcutaneous and infrapatellar adipose tissues, and evaluate the impact of cryopreservation on the cells. Based on paired comparisons of fresh and cryopreserved ASCs, cryopreserved ASCs had lower CD29 and CD44 protein expression and lower proliferation rates. The cryopreserved ASCs had relative lower mitochondria in the cytoplasm compared to the fresh ASCs regardless of tissue sources. …