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Full-Text Articles in Veterinary Medicine
Comparative Roles Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoproteins In Cytoplasmic Virion Egress, Hyuncheol Lee
Comparative Roles Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoproteins In Cytoplasmic Virion Egress, Hyuncheol Lee
LSU Master's Theses
HSV-1 acquires its final envelope by budding into cytoplasmic vesicles thought to be derived from Trans-Golgi Network (TGN) membranes. This process is facilitated by interactions among the carboxyl termini of viral glycoproteins and tegument proteins. To investigate the relative importance of different viral glycoproteins in cytoplasmic virion morphogenesis, a set of recombinant viruses were constructed silencing expression of the glycoprotein E (ΔgE), the carboxyl terminus of glycoprotein D (gDΔcp), and the membrane protein UL20 (ΔUL20). In addition, recombinant viruses were constructed having the ΔgE+gDΔcp, and the ΔgE+ΔgM (glycoprotein M) deletions. These recombinant viruses were constructed using the double-red, site-directed mutagenesis …
Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong
Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong
LSU Master's Theses
A conditional silencing system using anti-gB siRNAs was devised to investigate the structure and function of the KSHV gB. Transient co-transfection of plasmids constitutively expressing gB and anti-gB siRNAs in 293 cells substantially inhibited gB mRNA levels and protein production. Similarly, transient expression of siRNAs into the basal cavity-based lymphoma cells (BCBL-1) caused substantial reduction of gB transcription and protein synthesis. TaqMan real-time PCR and infectivity assays showed that gB was essential for virion egress and infectivity. Transfection of a codon-optimized gB not recognized by the anti-gB siRNAs, efficiently rescued virion egress and infectivity in BCBL-1 cells in the presence …
Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee
Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee
LSU Master's Theses
Identification of synoviocytes surrounding the canine cranial cruciate ligament (CrCL) has not been investigated. Objectives 1) Develop and validate a technique to identify and quantify normal canine CrCL associated synoviocytes. 2) Compare synoviocyte phenotype proportions surrounding normal and abnormal canine CrCLs Design In vitro experimental Animals Cranial cruciate ligaments from 4 intact female and 6 intact adult male mixed-breed dogs (objective 1) and from 8 adult female hound dogs (objective 2) Methods Objective 1) Normal CrCLs - CD18 and HSP25 epitopes were colocalized using immunohistochemistry. Sagittal sections were quantified in the proximal, middle, and distal aspects of each section. Western …
Effects Of Centrifugation On Equine Spermatozoa Immediately And After Cooling For 24 Hours, Jose Augusto Len
Effects Of Centrifugation On Equine Spermatozoa Immediately And After Cooling For 24 Hours, Jose Augusto Len
LSU Master's Theses
Len, Jose Augusto, MVZ, Universidad de Guadalajara, 1994 Master of Science, Summer Commencement, 2008 Major: Veterinary Medical Sciences Effects of Centrifugation on Equine Spermatozoa Immediately and After Cooling for 24 Hours Thesis directed by Professor Bruce Eilts Pages in thesis, 60. Words in abstract, 245. ABSTRACT The objectives of this study were to determine the effects of centrifugation on equine sperm progressive motility, plasma membrane integrity (viability), and acrosome integrity. We hypothesized that high centrifugation forces would be detrimental to equine sperm, yet recovery rates would increase. Ejaculates from six stallions were collected, extended (INRA96) to a concentration of 25 …
Randomized Controlled Trial Evaluating Adeno-Momp And Momp Dna Vaccines Against Chlamydophila Psittaci Challenge In Cockatiels (Nymphicus Hollandicus), David Sanchez-Migallon Guzman
Randomized Controlled Trial Evaluating Adeno-Momp And Momp Dna Vaccines Against Chlamydophila Psittaci Challenge In Cockatiels (Nymphicus Hollandicus), David Sanchez-Migallon Guzman
LSU Master's Theses
Chlamydophila psittaci causes severe disease in birds and humans, and important economical losses in the avian companion and poultry industry. Vaccines are the most cost-effective measure to control and help prevent infectious diseases, but to date there is no commercial vaccine available. A randomized clinical trial was conducted to assess the efficacy of two recombinant DNA vaccines against C. psittaci in Cockatiels (Nymphicus hollandicus). The first recombinant DNA vaccine has a gene encoding MOMP and the immunostimulant chitosan (MOMP DNA vaccine). The second recombinant DNA vaccine, contained the gene encoding MOMP that was vectored by a replication defective human adenovirus …