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Full-Text Articles in Veterinary Medicine
Plasmid-Mediated Expression Of Foreign Genes In Ehrlichia Canis, Langston Dolphus Hull
Plasmid-Mediated Expression Of Foreign Genes In Ehrlichia Canis, Langston Dolphus Hull
LSU Doctoral Dissertations
Ehrlichia canis is the etiologic agent of “tropical canine pancytopenia”, or canine ehrlichiosis. The impetus for this research was to overcome the lack of any reliable means of elucidating the genetic profiles of these illusive and historically difficult to manipulate organisms. The use of a broad-host range plasmid greatly facilitated the determination of an electro-transformation protocol. The transforming plasmid possesses a chloramphenicol antibiotic resistance gene marker (chloramphenicol acetyltransferase [CAT] gene), and a visual reporter gene marker, the Green Fluorescent Protein (GFP) gene. With primer sets designed to specifically amplify these two plasmid encoded gene markers, thus verifying the presence of …
Pathogenic Mechanisms Of Photobacterium Damselae Subspecies Piscicida In Hybrid Striped Bass, Ahmad A. Elkamel
Pathogenic Mechanisms Of Photobacterium Damselae Subspecies Piscicida In Hybrid Striped Bass, Ahmad A. Elkamel
LSU Doctoral Dissertations
Photobacterium damselae subspecies piscicida, previously known as Pasteurella piscicida, is an important pathogen of hybrid striped bass and many fish species cultured in brackish water in the United States, Japan, Europe, and the Mediterranean. The purpose of this study is to investigate virulence mechanisms that contribute to the pathogenesis of this organism. The ability of P. damselae to survive/replicate within hybrid striped bass macrophages was evaluated with an in vitro killing assay. Results indicated that the numbers of bacteria recovered from macrophages at 3, 6, 12, and 18 hours of incubation increased significantly over time. In contrast, the numbers of …