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Full-Text Articles in Veterinary Medicine
Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong
Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong
LSU Master's Theses
A conditional silencing system using anti-gB siRNAs was devised to investigate the structure and function of the KSHV gB. Transient co-transfection of plasmids constitutively expressing gB and anti-gB siRNAs in 293 cells substantially inhibited gB mRNA levels and protein production. Similarly, transient expression of siRNAs into the basal cavity-based lymphoma cells (BCBL-1) caused substantial reduction of gB transcription and protein synthesis. TaqMan real-time PCR and infectivity assays showed that gB was essential for virion egress and infectivity. Transfection of a codon-optimized gB not recognized by the anti-gB siRNAs, efficiently rescued virion egress and infectivity in BCBL-1 cells in the presence …