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Articles 1 - 5 of 5
Full-Text Articles in Veterinary Medicine
Novel Oncolytic Herpesviruses For Breast Cancer Treatment, Anna Israyelyan
Novel Oncolytic Herpesviruses For Breast Cancer Treatment, Anna Israyelyan
LSU Doctoral Dissertations
Oncolytic herpes simplex virus type-1 (HSV-1) has shown great potential as an effective agent for cancer therapy ¨C oncolytic virotherapy. To enhance oncolytic capabilities of previously reported agents, new oncolytic and fusogenic HSV-1 OncSyn and OncdSyn viruses were constructed based on wild type HSV-1 (F) strain. To provide for safety and tumor selectivity, the viruses carried a large deletion including one of the two ¦Ã134.5 genes. The ¦Ã134.5 gene, a major neurovirulence factor, was replaced by a gene cassette constitutively expressing the red fluorescent protein. Homologous recombination was utilized to transfer the fusogenic gBsyn3 mutation to the viral genome to …
Phylogenetics And New Approaches To Vaccine Development For West Nile, Arun Venkatesh Iyer
Phylogenetics And New Approaches To Vaccine Development For West Nile, Arun Venkatesh Iyer
LSU Doctoral Dissertations
West Nile virus (WNV) was first isolated in 1937 from a febrile Ugandan woman. WNV now has a worldwide distribution from Australia and India in the east to Europe and the United States in the west. The first human cases of WNV in the United States were detected in New York in 1999. The North American isolates were also found to be highly neuroinvasive and neurovirulent and in many cases leading to mortality or permanent CNS sequelae among humans. WNV was first isolated in Louisiana in 2001. The genome of this virus named LSU-AR01was sequenced and a detailed genetic analysis …
Comparative Roles Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoproteins In Cytoplasmic Virion Egress, Hyuncheol Lee
Comparative Roles Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoproteins In Cytoplasmic Virion Egress, Hyuncheol Lee
LSU Master's Theses
HSV-1 acquires its final envelope by budding into cytoplasmic vesicles thought to be derived from Trans-Golgi Network (TGN) membranes. This process is facilitated by interactions among the carboxyl termini of viral glycoproteins and tegument proteins. To investigate the relative importance of different viral glycoproteins in cytoplasmic virion morphogenesis, a set of recombinant viruses were constructed silencing expression of the glycoprotein E (ΔgE), the carboxyl terminus of glycoprotein D (gDΔcp), and the membrane protein UL20 (ΔUL20). In addition, recombinant viruses were constructed having the ΔgE+gDΔcp, and the ΔgE+ΔgM (glycoprotein M) deletions. These recombinant viruses were constructed using the double-red, site-directed mutagenesis …
The Zinc Induction Of Heme Oxygenase-1 Gene Promoter In Mouse Hepa Cells, Salim M. Alawneh
The Zinc Induction Of Heme Oxygenase-1 Gene Promoter In Mouse Hepa Cells, Salim M. Alawneh
LSU Doctoral Dissertations
Heme oxygenase-1 (HO-1) catalyzes the initial and rate-limiting reaction in heme catabolism (yielding equimolar quantities of iron, carbon monoxide, and biliverdin), which is further metabolized to bilirubin by biliverdin reductase. HO-1, an important enzyme with antioxidant and cytoprotective properties, functions against oxidative stress generated in response to heme and to various stress stimuli, such as zinc. The cytoprotective function of HO-1 is dependent on both the activities of the reaction products and on the inducibility of the heme oxygenase gene (hmox-1), which is regulated primarily at the level of gene transcription by two distal enhancers, E1 and E2. Characterization of …
Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong
Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong
LSU Master's Theses
A conditional silencing system using anti-gB siRNAs was devised to investigate the structure and function of the KSHV gB. Transient co-transfection of plasmids constitutively expressing gB and anti-gB siRNAs in 293 cells substantially inhibited gB mRNA levels and protein production. Similarly, transient expression of siRNAs into the basal cavity-based lymphoma cells (BCBL-1) caused substantial reduction of gB transcription and protein synthesis. TaqMan real-time PCR and infectivity assays showed that gB was essential for virion egress and infectivity. Transfection of a codon-optimized gB not recognized by the anti-gB siRNAs, efficiently rescued virion egress and infectivity in BCBL-1 cells in the presence …