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Full-Text Articles in Translational Medical Research
Anaplastic Thyroid Cancer: Identification Of Candidate Therapeutics And Mechanisms Of Disease Progression, Nicole Pinto
Anaplastic Thyroid Cancer: Identification Of Candidate Therapeutics And Mechanisms Of Disease Progression, Nicole Pinto
Electronic Thesis and Dissertation Repository
Malignancies derived from follicular cells of the thyroid can be divided into well-differentiated thyroid cancer (WDTC), poorly differentiated thyroid cancer and anaplastic (undifferentiated) thyroid cancer (ATC). While the majority of thyroid cancers are well-differentiated in nature and have an excellent prognosis, ATC is rare, nonresponsive to conventional therapies, and is nearly universally fatal. In this study, I employed high-throughput screening of kinase inhibitors to identify candidate drugs to control ATC and expanded these studies to include a focus on studying the underlying progression from WDTC to ATC. Two compounds, lestaurtinib (JAK2 inhibitor) and flavopiridol (pan-CDK inhibitor) were selected from the …
High Throughput Screening For Drug Discovery In Head And Neck Squamous Cell Carcinoma, Morgan D. Black
High Throughput Screening For Drug Discovery In Head And Neck Squamous Cell Carcinoma, Morgan D. Black
Electronic Thesis and Dissertation Repository
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide and despite advancements in traditional therapies, the survival rate of ~40% remains unchanged, thus highlighting the need for novel treatments in HNSCC. High throughput robotic screening of a panel of HNSCC cell lines was carried out against 1,505 compounds, where drug activity was measured using metabolic agent alamarBlue and quantified by percent activity. Initial screening found that the majority of active compounds could be grouped based upon their cellular target(s) and/or function including: cell cycle regulation, cytoskeleton disruption, and DNA topoisomerase function. Potency was confirmed with …