Occupational Health and Industrial Hygiene Commons^™

Surface Modification Of Biodegradable Microparticles With The Novel Host-Derived Immunostimulant Cpdi-02 Significantly Increases Short-Term And Long-Term Mucosal And Systemic Antibodies Against Encapsulated Protein Antigen In Young Naïve Mice After Respiratory Immunization, Jacob E. Parriott, Jason P. Stewart, D. David Smith, Stephen M. Curran, Christopher D. Bauer, Todd A. Wyatt, Joy A. Phillips, Elizabeth Lyden, Geoffrey M. Thiele, Joseph A. Vetro Phd

Journal Articles: Environmental, Agricultural & Occupational Health

Generating long-lived mucosal and systemic antibodies through respiratory immunization with protective antigens encapsulated in nanoscale biodegradable particles could potentially decrease or eliminate the incidence of many infectious diseases, but requires the incorporation of a suitable mucosal immunostimulant. We previously found that respiratory immunization with a model protein antigen (LPS-free OVA) encapsulated in PLGA 50:50 nanoparticles (~380 nm diameter) surface-modified with complement peptide-derived immunostimulant 02 (CPDI-02; formerly EP67) through 2 kDa PEG linkers increases mucosal and systemic OVA-specific memory T-cells with long-lived surface phenotypes in young, naïve female C57BL/6 mice. Here, we determined if respiratory immunization with LPS-free OVA encapsulated in …

Post-Endotoxin Exposure-Induced Lung Inflammation And Resolution Consequences Beneficially Impacted By Lung-Delivered Il-10 Therapy, Jill A. Poole, Rohit Gaurav, Aaron Schwab, Amy J. Nelson, Angela Gleason, Debra J. Romberger, Todd A. Wyatt

Journal Articles: Environmental, Agricultural & Occupational Health

Although lung diseases typically result from long-term exposures, even a robust, one-time exposure can result in long-lasting consequences. Endotoxin is a ubiquitous environmental/occupational inflammatory agent often used to model airway inflammation. Using a murine model, the return to lung homeostasis following high dose inhalant lipopolysaccharide (LPS, 10-100 μg) exposure were delineated over 2 weeks. LPS-induced rapid weight loss, release of proinflammatory mediators, and inflammatory cell influx with prolonged persistence of activated macrophages CD11c⁺CD11b⁺ and recruited/transitioning CD11c^intCD11b⁺ monocyte-macrophages out to 2 weeks. Next, lung-delivered recombinant (r) interleukin (IL)-10 was intratracheally administered for 3 doses initiated …

Covid-19 Patients With Documented Alcohol Use Disorder Or Alcohol-Related Complications Are More Likely To Be Hospitalized And Have Higher All-Cause Mortality, Kristina L. Bailey, Harlan Sayles, James Campbell, Neha Khalid, Madyson Anglim, Jana Ponce, Todd A. Wyatt, James C. Mcclay, Ellen L. Burnham, Alfred Anzalone, Corrine K. Hanson

Journal Articles: Environmental, Agricultural & Occupational Health

BACKGROUND: Coronavirus Disease 2019 (COVID-19) has affected every country globally, with hundreds of millions of people infected with the SARS-CoV-2 virus and over 6 million deaths to date. It is unknown how alcohol use disorder (AUD) affects the severity and mortality of COVID-19. AUD is known to increase the severity and mortality of bacterial pneumonia and the risk of developing acute respiratory distress syndrome. Our objective is to determine whether individuals with AUD have increased severity and mortality from COVID-19.

METHODS: We utilized a retrospective cohort study of inpatients and outpatients from 44 centers participating in the National COVID Cohort …

Characterization Of Aerosols Produced By Common Orthodontic Procedures, Anna R. Schoettger

Theses & Dissertations

Introduction: Aerosols can harbor active viruses, including SARS-CoV-2, making it vital that clinical protocols implemented in the orthodontic office be based on which procedures produce aerosols. Research shows that various procedures in the field of orthodontics generate aerosols, however, there is a lack of consensus as to which procedures should be deemed aerosol-generating procedures (AGPs). The current study aimed to better understand aerosol production of various orthodontic procedures. Methods: Orthodontic procedures were performed in a closed-room dental operatory on acrylic teeth mounted onto a dental manikin. Procedures performed included: debonding, bonding, and repositioning of one bracket. Variations introduced included: high-speed …

Occupational Injuries Associated With Workplace Violence In An Academic Medical Center, Rebecca J. Bonacci

Capstone Experience: Master of Public Health

Violence is a major source of workplace injury in the medical profession. Peer reviewed literature is lacking for workers that have injuries through violence from clients who have intellectual disabilities. Literature for hospital security is also lacking. In this study of workplace violence, we compared injuries among the occupational groups at an academic medical center from 2017-2020. We hypothesized that violence will be similar across the years. Occupational injury and illness data was obtained from an academic medical center. The number of violence cases was divided by the total number of injury cases and reported as a percentage. We found …

Developing And Piloting An E-Learning Module On Respiratory Protection, Emily Girard

Capstone Experience: Master of Public Health

Respiratory diseases are one of the most common causes of occupational illnesses in the United States. Many occupations use personal protective equipment (PPE) in the form of respirators to protect against respiratory hazards when other control methods are not effective. However, some workers may not have had adequate training on their use. An e-learning module was developed to provide information about respirators and their use in agriculture. The interactions of matching, sorting, and review questions require the user to be an active participant with the goal of reinforcing the information. It was hypothesized the average summative assessment score would increase …

Neutralization Of Il-33 Modifies The Type 2 And Type 3 Inflammatory Signature Of Viral Induced Asthma Exacerbation, Kristi J. Warren, Jill A. Poole, Jenea M. Sweeter, Jane M. Devasure, John D. Dickinson, R. Stokes Peebles, Todd A. Wyatt

Journal Articles: Environmental, Agricultural & Occupational Health

BACKGROUND: Respiratory viral infections are one of the leading causes of need for emergency care and hospitalizations in asthmatic individuals, and airway-secreted cytokines are released within hours of viral infection to initiate these exacerbations. IL-33, specifically, contributes to these allergic exacerbations by amplifying type 2 inflammation. We hypothesized that blocking IL-33 in RSV-induced exacerbation would significantly reduce allergic inflammation.

METHODS: Sensitized BALB/c mice were challenged with aerosolized ovalbumin (OVA) to establish allergic inflammation, followed by RSV-A2 infection to yield four treatment groups: saline only (Saline), RSV-infected alone (RSV), OVA alone (OVA), and OVA-treated with RSV infection (OVA-RSV). Lung outcomes included …

Nuclear Factor Kappa-B Contributes To Cigarette Smoke Tolerance In Pancreatic Ductal Adenocarcinoma Through Cysteine Metabolism, Venugopal Gunda, Yashpal S Chhonker, Nagabhishek Sirpu Natesh, Pratima Raut, Sakthivel Muniyan, Todd A. Wyatt, Daryl J. Murry, Surinder K. Batra, Satyanarayana Rachagani

Journal Articles: Environmental, Agricultural & Occupational Health

BACKGROUND: Retrospective studies revealed that cigarette smoking enhances risk of incidence and worsens prognosis in pancreatic cancer (PC) patients. Poor prognosis in smoker cohort of PC patients indicates prevalence of cigarette smoke stimulated survival mechanisms yet to be explored in PC. In this study, cigarette smoke induced metabolic pathways were explored and targeted in PC.

METHODS: Human pancreatic ductal adenocarcinoma cell (PDAC) lines, genetically engineered mice models (GEMMs), mass spectrometry based heavy isotope-based metabolite analysis, cytotoxicity assays and Nuclear factor kappa-B (NF-kB) targeting were utilized in this study. Cigarette smoke extract (CSE) was prepared fresh each day by bubbling cell …

Development Of A Web-Based Agriculture Health Risk Assessment Tool For Military Veteran Farmers And Ranchers, Alyssa Damke

Capstone Experience: Master of Public Health

In the U.S., significant health disparities exist among rural populations compared to their urban counterparts. These disparities can be compounded in agricultural workers due to the dangerous and unpredictable nature of their work. Agricultural producers who are also military Veterans may experience additional health pressures that are often overlooked in the healthcare setting. Over 2.5 million Veteran Health Administration (VHA) patients reside in rural areas and the healthcare infrastructure to care for these Veterans may not include an agricultural occupational approach. To address healthcare disparities in Veteran farmers and ranchers, this study, in collaboration with AgriSafe Network, will be used …

Planning And Facilitating The Training Of Emergency Medical Services On The Safe Transport Of Patients With Highly Infectious Diseases, Mohammed Alhallaf

Theses & Dissertations

Emergency Medical Services (EMS) personnel are the first line of treatment and are the first exposed health care workers to infectious diseases (IDs). Consequently, the EMS setting is a relatively vulnerable environment for disease transmission and cross-contamination. Also, serious and emerging IDs, such as highly pathogenic viral hemorrhagic fevers and novel Coronaviruses, in a time of increased globalization, require comprehensive EMS training and preparedness. This project had the objective of identifying best practices for the EMS transport of patients with IDs. The methods for this project were done in two phases. In the first stage, we …

Dual Substance Use Of Electronic Cigarettes And Alcohol, Tanner J. Wetzel, Todd A. Wyatt

Journal Articles: Environmental, Agricultural & Occupational Health

Electronic cigarettes (ECs) are a modern nicotine delivery system that rapidly grew in widespread use, particularly in younger populations. Given the long history of the comorbidity of alcohol and nicotine use, the rising prevalence of ECs raises the question as to their role in the consumption of alcohol. Of the numerous models of ECs available, JUUL is the most popular. This narrative review aims to determine current trends in literature regarding the relationship between EC and alcohol dual use, as well as hypothesize potential pathogenic tissue damage and summarize areas for future study, including second-hand vapor exposure and calling for …

Cs-Cash Pilot Grant: Improving Agricultural Worker Health & Safety Awareness Through Multimodal, Case-Based Physician Assistant Education, Carey Wheelhouse, Mia Hyde, Annie Wildermuth, Wayne Mathews

Posters and Presentations: Physician Assistant

The University of Nebraska Medical Center Division of Physician Assistant Education intends to improve health outcomes of patients in Midwest agricultural communities through didactic, clinical, and case-based instruction of Physician Assistant (PA) students.

Aims

1. Increase PA student awareness of agricultural health and safety issues
2. Increase PA student medical knowledge on respiratory health, chemical exposure, and noise-induced hearing loss
3. Enhance PA student communication skills with agricultural patients
4. Increase patient health care access in agricultural environments

UNMC PA students will undergo learning specific to agricultural-related respiratory health issues, chemical exposures, and hearing loss, including preventative health education and communication with agricultural patient …

The Lived Experience Of Psychological Occupational Stress In Early Childhood Education, Brooke Fletcher

Capstone Experience: Master of Public Health

Early childhood education (ECE) programs have been recognized by the Institute of Medicine and the Administration for Children and Families as excellent interventions to alleviate the long-term negative effects of poverty in the United States, but the quality of intervention provided and outcomes for the target population are adversely hindered by high occupational stress burdens for ECE teachers. According to Sheridan, Edwards, Marvin, and Knoche, the models used to address this concern are not empirically supported for specific applicability to ECE nonprofit settings. There is a need for deeper understanding of the lived experiences of ECE teachers, including the psychological …

Initiation Of A Roundtable Meeting To Determine Safety Hazards And Provide Education To Range Bison Herd Workers, Lucia Finocchiaro

Capstone Experience: Master of Public Health

This capstone project serves as an important piece of greater project studying bison handling. Bison production is both a growing and dangerous industry. As a new enterprise in many parts of the country and on tribal lands, significant numbers of untrained employees may be hired. This project undertakes methods to better understand the risks associated with bison handling and to thereby improve handling procedures and increase safety. Herd managers (from tribal and non-tribal sites), researchers, safety experts, and stakeholders were brought together for a roundtable meeting in Omaha. This meeting was designed to provide stakeholders a forum to discuss common …

Ovalbumin-Sensitized Mice Have Altered Airway Inflammation To Agriculture Organic Dust, Kristi J. Warren, John D. Dickinson, Amy J. Nelson, Todd A. Wyatt, Debra J. Romberger, Jill A. Poole

Journal Articles: Environmental, Agricultural & Occupational Health

Agriculture exposures are associated with reducing the risk of allergy and asthma in early life; yet, repeated exposures later in life are associated with chronic bronchitis and obstructive pulmonary diseases. The objective of this study was to investigate the airway inflammatory response to organic dust extract (ODE) in mice with established ovalbumin (OVA)-induced experimental asthma. C57BL/6 mice were either OVA sensitized/aerosol-exposed or saline (Sal) sensitized/aerosol-challenged. Both groups were then subsequently challenged once with intranasal saline or swine confinement ODE to obtain 4 treatment groups of Sal-Sal, Sal-ODE, OVA-Sal, and OVA-ODE. Airway hyper-responsiveness (AHR) to methacholine, bronchiolar lavage fluid, lung tissues, …

Hearing Loss And Hearing Protection Use Among Midwestern Farmers, Josie J. Ehlers

Theses & Dissertations

Many farmers have noise-induced hearing loss, yet few use hearing protection when working around loud noise. A point source intervention (storing hearing protection devices near sources of noise) was implemented to help farmers overcome accessibility barriers related to using hearing protection. Intervention farmers (n=53) received education and the point source intervention; control farmers (n=36) received education only. During each year of the study, all farmers completed a questionnaire about their perceptions of hearing protection and participated in an audiometric test at their farm. Ambient sound pressure levels were taken during tests. The main objectives of this dissertation were to evaluate …

An Assessment Of Preparations Made In The United States For Highly Hazardous Communicable Diseases Following The 2014-2016 Ebola Virus Disease Epidemic, Jocelyn J. Herstein

Theses & Dissertations

The 2014-2016 Ebola virus disease (EVD) epidemic in West Africa was unprecedented in magnitude and scope. The threat of imported cases of EVD in the United States prompted the Centers for Disease Control and Prevention (CDC) to establish a tiered network of hospitals to enhance domestic isolation capacity, including the designation of select hospitals as Ebola treatment centers (ETCs). As of spring 2015, no information existed on the capacity, physical infrastructure, staffing models, or infection control protocols of these newly-established ETCs, nor was there information on other highly hazardous communicable diseases (HHCDs) these units would admit. Moreover, no documentation was …

Hearing Conservation Program On A Campus: Worker Noise Exposure Assessment And Training, Tika Nepal

Capstone Experience: Master of Public Health

Abstract

Introduction: Campus workers are exposed to loud noise from boilers, chillers, generators, snow blowers, leaf blowers, helicopters, utility vehicles, laboratory animals etc. No studies have looked at characterizing noise exposures among campus workers to see who needed to be enrolled in the Hearing Conservation Program (HCP).

Goal: The goal of this study was to determine which employees needed to be enrolled in a HCP.

Methods: This was a cross sectional study conducted among campus workers. We identified utility plant workers, comparative medicine workers, landscaping crew, security officers and facility staff who used utility vehicles as employees potentially exposed to …

Occupational Health Challenges In Research, Anthony Blake

Capstone Experience: Master of Public Health

ABSTRACT

Introduction: Employees working in universities and hospitals are faced with common and unique health and safety challenges. Usually, the campus Department of Health and Safety is tasked with reducing employee exposure through risk assessments. Purpose: In this study, we present a unique risk assessment case study related to a research project undertaken by the campus Department of Health and Safety (DHES). This project also describes the activities done by DHES to improve campus safety. We hypothesized that carbon monoxide, particulate matter, formaldehyde, and VOCs emitted from burning wood chips in a closed laboratory space will exceed …

Organic Barn Dust Inhibits Surfactant Protein D Production Through Protein Kinase-C Alpha Dependent Increase Of Gpr116, David Schneberger, Jane M. Devasure, Shelley A. Kirychuk, Todd A. Wyatt

Journal Articles: Environmental, Agricultural & Occupational Health

Prolonged exposure to organic barn dusts can lead to chronic inflammation and a broad range of lung problems over time, mediated by innate immune mechanisms. The immune surfactant or collectin surfactant protein D (SP-D) is a crucial multifunctional innate immune receptor. Little work to date has examined the effect of such collectins in response to organic dusts. We provide evidence here that agricultural organic dusts can inhibit mRNA and protein expression of SP-D in a human alveolar epithelial cell line, and an in vivo mouse model. This inhibition was not a result of lipopolysaccharide (LPS) or peptidoglycans, the two most …

Malondialdehyde-Acetaldehyde (Maa) Adducted Surfactant Protein Induced Lung Inflammation Is Mediated Through Scavenger Receptor A (Sr-A1)., Muna Sapkota, Jane M. Devasure, Kusum K. Kharbanda, Todd A. Wyatt

Journal Articles: Environmental, Agricultural & Occupational Health

BACKGROUND: Co-exposure to cigarette smoke and alcohol leads to the generation of high concentrations of acetaldehyde and malondialdehyde in the lung. These aldehydes being highly electrophilic in nature react with biologically relevant proteins such as surfactant protein D (SPD) through a Schiff base reaction to generate SPD adducted malondialdehyde-acetaldehyde adduct (SPD-MAA) in mouse lung. SPD-MAA results in an increase in lung pro-inflammatory chemokine, keratinocyte chemoattractant (KC), and the recruitment of lung lavage neutrophils. Previous in vitro studies in bronchial epithelial cells and macrophages show that scavenger receptor A (SR-A1/CD204) is a major receptor for SPD-MAA. No studies have yet examined …

Surveillance Of Non-Fatal Agricultural Injuries Among Farm Operators In The Central States Region Of The United States, Ketki Patel

Theses & Dissertations

Agriculture is a major industry in the U.S. with high rates of fatal and non-fatal occupational injuries. The dynamic nature of the U.S. agriculture industry, regional variations in farming practices, and the diverse workforce make surveillance of injuries challenging. A recent National Academies (U.S.) evaluation reported that data for non-fatal agricultural injury are scarce, and mainly available through national surveys. Limited data are available for employees in the agriculture sector, especially farm owners and operators. The objectives of this study were to- 1) review and evaluate existing survey-based systems for surveillance of non-fatal agricultural injuries on U.S. farms, and 2) …

Risk Factors For Agricultural Injury: An Evaluation Using Systematic Review And Injury Surveillance, Rohan Mahadeo Jadhav

Theses & Dissertations

Purpose- Agriculture is the most hazardous industry in the United States. The effectiveness of intervention programs for injury prevention can be improved by acquiring knowledge of risk factors for occupational injury in agricultural operators. The landscape of agriculture is changing in the U.S. Agricultural populations, environments and risk factors are changing as well with the changes in the structure of farms and ranches. The objective of this study was to identify significant risk factors for agricultural injury based on the literature and three years of injury surveillance data covering seven U.S. states. Methods- We conducted a systematic review of reported …