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Environmental Public Health Commons™

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University of Nevada, Las Vegas

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Articles 61 - 66 of 66

Full-Text Articles in Environmental Public Health

Determination Of The Efficacy Of Two Building Decontamination Strategies By Surface Sampling With Culture And Quantitative Pcr Analysis, Mark P. Buttner, Patrica Cruz, Linda Stetzenbach, Amy Klima-Comba Aug 2004

Determination Of The Efficacy Of Two Building Decontamination Strategies By Surface Sampling With Culture And Quantitative Pcr Analysis, Mark P. Buttner, Patrica Cruz, Linda Stetzenbach, Amy Klima-Comba

Environmental & Global Health Faculty Research

The efficacy of currently available decontamination strategies for the treatment of indoor furnishings contaminated with bioterrorism agents is poorly understood. Efficacy testing of decontamination products in a controlled environment is needed to ensure that effective methods are used to decontaminate domestic and workplace settings. An experimental room supplied with materials used in office furnishings (i.e., wood laminate, painted metal, and vinyl tile) was used with controlled dry aerosol releases of endospores of Bacillus atrophaeus (“Bacillus subtilis subsp. niger,” also referred to as BG), a Bacillus anthracis surrogate. Studies were performed using two test products, a foam decontaminant and chlorine dioxide …

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Enhanced Detection Of Surface-Associated Bacteria In Indoor Environments By Quantitative Pcr, Mark P. Buttner, Patrica Cruz-Perez, Linda Stetzenbach Jun 2001

Enhanced Detection Of Surface-Associated Bacteria In Indoor Environments By Quantitative Pcr, Mark P. Buttner, Patrica Cruz-Perez, Linda Stetzenbach

Environmental & Global Health Faculty Research

Methods for detecting microorganisms on surfaces are needed to locate biocontamination sources and to relate surface and airborne concentrations. Research was conducted in an experimental room to evaluate surface sampling methods and quantitative PCR (QPCR) for enhanced detection of a target biocontaminant present on flooring materials. QPCR and culture analyses were used to quantitateBacillus subtilis (Bacillus globigii) endospores on vinyl tile, commercial carpet, and new and soiled residential carpet with samples obtained by four surface sampling methods: a swab kit, a sponge swipe, a cotton swab, and a bulk method. The initial data showed that greater overall sensitivity was obtained …

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Pcr For Bioaerosol Monitoring: Sensitivity And Environmental Interference, Aj Alvarez, Mark P. Buttner, Linda Stetzenbach Jan 1995

Pcr For Bioaerosol Monitoring: Sensitivity And Environmental Interference, Aj Alvarez, Mark P. Buttner, Linda Stetzenbach

Environmental & Global Health Faculty Research

The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were collected with AGI-30 samplers and seeded with E. coli DH1 cells. Parallel studies were performed with cells seeded into the sampler prior to collection of air samples to determine the effects of environmental inhibition and sampling stress on the PCR assay. Baseline studies were also performed without environmental challenge or sampling stress to compare two protocols …

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Analysis And Evaluation Of The Workplace Exposure Assessment Workbook Developed By Keith Tait Including Some Recommended Revisions, Lisa Hebberd Oct 1993

Analysis And Evaluation Of The Workplace Exposure Assessment Workbook Developed By Keith Tait Including Some Recommended Revisions, Lisa Hebberd

UNLV Theses, Dissertations, Professional Papers, and Capstones

Some risk due to the contamination of workplace environments is an inevitable part of human lives. These risks can often be reduced by improving the control of environmental pollution in the workplace. An extremely important challenge for any industry is to develop a mechanism to identify acceptable levels of safety, or "acceptable risk" in the workplace for specific situations, and to assure adequate quality control over measured or calculated exposure concentrations and their possible contributions to adverse health effects.

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Monitoring Of Fungal Spores In An Experimental Indoor Environment To Evaluate Sampling Methods And The Effects Of Human Activity On Air Sampling, Mark P. Buttner, Linda Stetzenbach Jan 1993

Monitoring Of Fungal Spores In An Experimental Indoor Environment To Evaluate Sampling Methods And The Effects Of Human Activity On Air Sampling, Mark P. Buttner, Linda Stetzenbach

Environmental & Global Health Faculty Research

Aerobiological monitoring was conducted in an experimental room to aid in the development of standardized sampling protocols for airborne microorganisms in the indoor environment. The objectives of this research were to evaluate the relative efficiencies of selected sampling methods for the retrieval of airborne fungal spores and to determine the effect of human activity on air sampling. Dry aerosols containing known concentrations of Penicillium chrysogenum spores were generated, and air samples were taken by using Andersen six-stage, Surface Air System, Burkard, and depositional samplers. The Andersen and Burkard samplers retrieved the highest numbers of spores compared with the measurement standard, …

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Evaluation Of Four Aerobiological Sampling Methods For The Retrieval Of Aerosolized Pseudomonas Syringae, Mark P. Buttner, Linda Stetzenbach Apr 1991

Evaluation Of Four Aerobiological Sampling Methods For The Retrieval Of Aerosolized Pseudomonas Syringae, Mark P. Buttner, Linda Stetzenbach

Environmental & Global Health Faculty Research

The Andersen six-stage impactor, the SAS (Surface Air System) impactor, the AGI-30 impinger, and gravity plates were evaluated for the retrieval of aerosol-releasedPseudomonas syringae. The upper limits of the impactor samplers were exceeded at a spray concentration of 10^7 CFU/ml, indicating that these samplers are not appropriate for monitoring high airborne concentrations. Decreased cell concentrations were retrieved with increased sampling time for the Andersen and AGI samplers, indicating that a minimum sampling time is preferable for monitoring aerosolized vegetative cells.

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