Pharmacy and Pharmaceutical Sciences Commons^™

Adenosine 3', 5'-Cyclic Monophosphate Activation Of Islet Chloride Channels, Anjali Varandani

Theses and Dissertations

The objective of this thesis was to understand the regulation of islet Cl⁻ current by cAMP. This current, known as I_cl,islet flew is the first Cl⁻ channel current characterized in pancreatic �� cells. I_cl,islet has been hypothesized to modulate insulin secretion through changes in islet electrical activity. Both 5 ��M forskolin and 100 ��M IBMX (3-isobutyl-1-methylxanthine), agents that increase intracellular cAMP, were shown to activate an outwardly-rectifying ionic current in HIT cells that closely resembled I_cl,islet. The current was blocked when iodide was substituted for external Cl⁻ or when the Cl⁻ channel blocker niflumic acid was …