Pharmacy and Pharmaceutical Sciences Commons^™

Cross-Sectional Survey Of Perceived Barriers Among Community Pharmacists Who Do Not Immunize, In Wayne County, Michigan, Sarah E. Kelling, Anthony Pattin, Abdulbaset Salim, Paul Kilgore, Steven R. Erickson

Department of Pharmacy Practice

Introduction: The goal of the study was to identify perceived barriers to implementation of vaccination services encountered by independent and small-chain community pharmacies in an urban setting.

Methods: Pharmacists in independent and small-chain pharmacies located in 29 Michigan ZIP codes were visited and asked to complete a 5- to 10-min semi-structured interview.

Results: A total of 93 independent and 12 small-chain pharmacies participated (n = 105; 61%). The pharmacies filled an average of 700 prescriptions each week with 1.1 pharmacist full-time equivalents and 57 h of technician time. The most common services that participating pharmacies provided were dispensing outpatient …

Engagement In A Diverse Urban Community To Describe Community Residents’ Perceptions Of Pharmacists As Immunizers, Anthony J. Pattin, Zachary Rowe, Paul Kilgore, Nada Farhat, Stephen Kaurala, Linda Kaljee

Department of Pharmacy Practice

Objective: To describe the perceptions of pharmacist-delivered vaccination services among community members that live in a diverse urban metropolitan area and to identify potential strategies to improve the use of vaccines provided by pharmacists.

Design: Qualitative study. Setting: Metropolitan urban diverse community in Wayne County, MI.

Participants: Individuals 50 years of age and older that reside in a defined urban metropolitan community.

Intervention: Four, 45-minute, focus group discussions were conducted to gather the experiences and perceptions of participants around pharmacists that immunize. A focus group guide was developed to facilitate focus group sessions.

Main Outcome Measures: Focus group discussions were …

Characterization Of Α‑Synuclein Multimer Stoichiometry In Complex Biological Samples By Electrophoresis, Bryan A. Killinger, Anna Moszczynska

Pharmaceutical Sciences Faculty Publications

The aberrant aggregation of α-synuclein in the brain is a hallmark of Parkinson’s disease (PD). In vivo soluble α-synuclein occurs as a monomer and several multimers, the latter of which may be important for the biological function of α-synuclein. Currently, there is a lack of reproducible methods to compare α-synuclein multimer abundance between complex biological samples. Here we developed a method, termed “multimer-PAGE,” that combines in-gel chemical cross-linking with several common electrophoretic techniques to measure the stoichiometry of soluble α-synuclein multimers in brain tissue lysates. Results show that soluble α-synuclein from the rat brain exists as several high molecular weight …

Chao Yuanfang: Imperial Physician Of The Sui Dynasty And An Early Pertussis Observer?, Yan Liang, Abdulbaset M. Salim, Wendy Wu, Paul E. Kilgore

Department of Pharmacy Practice

Early Chinese texts contain extensive disease descriptions, including various texts that contain descriptions of modernday conditions. During the Sui Dynasty, a leading scholar, Chao Yuanfang, may have authored a leading treatise 1400 years ago. Although these texts are the subject of ongoing research, evidence suggests that a clinical syndrome consistent with pertussis was observed in ancient China.

A Novel Loop-Mediated Isothermal Amplification Assay For Serogroup Identification Of Neisseria Meningitidis In Cerebrospinal Fluid, Dokyung Lee, Eun Jin Kim, Paul E. Kilgore, Hideyuki Takahashi, Jun Tomono, Shigehiko Miyamoto, Daisuke Omagari, Dong Wook Kim, Mitsuko Seki

Department of Pharmacy Practice

We have developed a novel Neisseria meningitidis serogroup-specific loop-mediated isothermal amplification (LAMP) assay for six of the most common meningococcal serogroups (A, B, C, W, X, and Y). The assay was evaluated using a set of 31 meningococcal LAMP assay positive cerebrospinal fluid (CSF) specimens from 1574 children with suspected meningitis identified in prospective surveillance between 1998 and 2002 in Vietnam, China, and Korea. Primer specificity was validated using 15 N. meningitidis strains (including serogroups A, B, C, E, W, X, Y, and Z) and 19 non-N. meningitidis species. The N. meningitidis serogroup LAMP detected down to ten copies …