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In Vivo Spect/Ct Molecular Imaging Of Lfa-1 In Atherosclerotic Inflammatory Development With Radiolabeled Danbirt, Roberto Ivan Mota Alvidrez

Pharmaceutical Sciences ETDs

The objective of this thesis project is to assess inflammatory leukocyte presence and accumulation in vascular atherosclerotic plaque using [111In] In-DANBIRT as a non-invasive diagnostic imaging tool. ApoE-/-mice were fed either normal or high fat diet (HFD) for 8 weeks. Sprague Dawley male rats were exposed to filtered air and ozone for 4 hours. SPECT/CT imaging was performed at baseline, 4 and 8 weeks. 3D autoradiography was performed after 8 weeks. Leukocyte isolation methods were performed for neutrophil and peripheral blood mononuclear cell (PBMC) separations post-incubation with [68Ga] Ga-DANBIRT and [111In] In-DANBIRT. Whole blood isolation correlated these findings showing increased …