Open Access. Powered by Scholars. Published by Universities.®
Pharmacy and Pharmaceutical Sciences Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Pharmacy and Pharmaceutical Sciences
Analysis Of Oocyte-Like Cells Differentiated From Porcine Fetal Skin-Derived Stem Cells, Paul Dyce, Wei Shen, Evanna Huynh, Hua Shao, Daniel Villagómez, Gerald Kidder, W Allan King, Julang Li
Analysis Of Oocyte-Like Cells Differentiated From Porcine Fetal Skin-Derived Stem Cells, Paul Dyce, Wei Shen, Evanna Huynh, Hua Shao, Daniel Villagómez, Gerald Kidder, W Allan King, Julang Li
Paul W. Dyce
We previously reported the differentiation of cells derived from porcine female fetal skin into cells resembling germ cells and oocytes. A subpopulation of these cells expressed germ cell markers and formed aggregates resembling cumulus-oocyte complexes. Some of these aggregates extruded large oocyte-like cells (OLCs) that expressed markers consistent with those of oocytes. The objective of the current study was to further characterize OLCs differentiated from porcine skin-derived stem cells. Reverse transcriptase (RT)-polymerase chain reaction and Western blot revealed the expression of connexin37 and connexin43, both of which are characteristic of ovarian follicles. The expression of meiosis markers DMC1 and synaptonemal …