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Evaluation Of A Field-Deployable Reverse Transcription-Insulated Isothermal Pcr For Rapid And Sensitive On-Site Detection Of Zika Virus, Mariano Carossino, Yanqiu Li, Pei-Yu A. Lee, Chuan-Fu Tsai, Pin-Hsing Chou, Dennis Williams, Ashley Skillman, R. Frank Cook, Grayson Brown, Hsiao-Fen G. Chang, Hwa-Tang T. Wang, Udeni B. R. Balasuriya

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: The recent emergence of Zika virus (ZIKV) in Brazil and its precipitous expansion throughout the Americas has highlighted the urgent need for a rapid and reliable on-site diagnostic assay suitable for viral detection. Such point-of-need (PON), low-cost diagnostics are essential for ZIKV control in vulnerable areas with limited resources.

Methods: We developed and evaluated a ZIKV-specific field-deployable RT-iiPCR reagent set targeting the E gene for rapid detection of ZIKV in ZIKV-spiked human and mosquito specimens, and compared its performance to the Center for Disease Control and Prevention (CDC) and Pan American Health Organization (PAHO) RT-qPCR assays targeting the E …

Emergence Of The L Phenotype In Group B Streptococci In The South Of Ireland, Katherine Hayes, Lesley Cotter, L. Barry, Fiona O'Halloran

Department of Biological Sciences Publications

Group B Streptococcal isolates (n = 235) from the South of Ireland were characterised by serotyping, antimicrobial susceptibility and determination of the phenotypic and genotypic mechanisms of resistance. Resistance to erythromycin and clindamycin was observed in 21·3% and 20·4% of the total population, respectively. The c-MLSB phenotype was the most common phenotype detected (62%), with ermB being the predominant genetic determinant, present in 84% of resistant isolates. The rare L phenotype was observed in 2·9% (n = 7) of isolates, four of which harboured the lsaC gene responsible for clindamycin resistance. Serotypes Ia, III and II were the most common …

Puerto Rico : Zika Virus, Rachel Cybulski

Global Public Health

Puerto Rico has had an epidemic of Zika virus within the past few years. Considering the close distance to America, the U.S. has been up-to-date in stopping the spread of Zika from Puerto Rico to the states. In 2016 particularly, the United States declared Puerto Rico to be in a state of emergency due to the heavy presence of the virus. Although this specific outbreak has been terminated, the likelihood of another such occurrence is high, so it is important to learn about the effects of Zika and ways to prevent the spread. The major result of having the virus …

Burundi : Water Scarcity, Isabel Salas

Global Public Health

Burundi is located near the African great lakes region of East Africa. This country is one of the poorest and hungriest countries throughout the world because of this they are facing multiple problems with communicable disease. These families are seeing a reduction in the amount of water available to them and based on this they are receiving water from external sources such as wells and lakes. Most of the water throughout Burundi is contaminated with Phytoplankton which increase cholera outbreaks in this area. It has also been shown that uranium impacts the water throughout the wells and lakes. Both of …