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Full-Text Articles in Organisms
Analyzing Wisp Dna Sequences And Building Primers For Dna Separation, Paige Spicer, Payton Crum
Analyzing Wisp Dna Sequences And Building Primers For Dna Separation, Paige Spicer, Payton Crum
Scholars Day Conference
A bacteriophage is a virus that infects bacteria. Bacteriophage DNA was isolated from a lysate sample, but two separate, unrelated genome sequences were returned. Plaque assays and plaque streaking were used to in an effort to separate the phages. After plaque isolation was unsuccessful, NCBI BLAST and IDT software were used to build unique primers for each of the two unrelated genomes. After receiving the primers, PCR and gel electrophoresis were run on plaques and lysate from each phage to classify each phage in the correct cluster and sub cluster as identified by the original DNA sequencing.
Using Oncodb's Oncovirus Analysis For Hbv-Related Hcc, Anna Claire Newman
Using Oncodb's Oncovirus Analysis For Hbv-Related Hcc, Anna Claire Newman
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Since one of the known causes of cancer is virus infection causing changes in gene regulation, being able to compare the expression and methylation of regular tumors and virus-related tumors could provide much needed information into virus induced cancer such as HBV-related HCC. OncoDB's oncovirus analysis capabilities can provide information and research opportunities that past research has not had.
Progress Towards Phage Stability For Possible Oral Phage Therapy, Erin Russo
Progress Towards Phage Stability For Possible Oral Phage Therapy, Erin Russo
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Oral cavities or caries is a localized deterioration of the tooth caused by an accumulation of harmful bacteria. This research intends to integrate bacteriophages onto unwaxed dental floss as a possible future preventative treatment for oral cavities. Bacteriophages are viruses that degrade the composition of bacterial populations by invading and reproducing inside bacterial hosts. The experimental framework involves soaking unwaxed floss in the bacteriophages named Phrick and Stonehill. The effectiveness of infection is measured by applying the phage-infused floss on an agar plate with Gordonia terrae CAG3, then analyzing the diameter of the plaque around the floss. This research will …