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Capsid Structure Of Dsrna Fungal Viruses, Daniel Luque, Carlos P. Mata, Nobuhiro Suzuki, Said A. Ghabrial, José R. Castón
Capsid Structure Of Dsrna Fungal Viruses, Daniel Luque, Carlos P. Mata, Nobuhiro Suzuki, Said A. Ghabrial, José R. Castón
Plant Pathology Faculty Publications
Most fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles involved in the organization of the viral genome and the viral polymerase necessary for RNA synthesis. The atomic structure of the T = 1 capsids of four mycoviruses was resolved: the L-A virus of Saccharomyces cerevisiae (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix …
Acquisition Of Functions On The Outer Capsid Surface During Evolution Of Double-Stranded Rna Fungal Viruses, Carlos P. Mata, Daniel Luque, Josué Gómez-Blanco, Javier M. Rodríguez, José M. González, Nobuhiro Suzuki, Said A. Ghabrial, José L. Carrascosa, Benes L. Trus, José R. Castón
Acquisition Of Functions On The Outer Capsid Surface During Evolution Of Double-Stranded Rna Fungal Viruses, Carlos P. Mata, Daniel Luque, Josué Gómez-Blanco, Javier M. Rodríguez, José M. González, Nobuhiro Suzuki, Said A. Ghabrial, José L. Carrascosa, Benes L. Trus, José R. Castón
Plant Pathology Faculty Publications
Unlike their counterparts in bacterial and higher eukaryotic hosts, most fungal viruses are transmitted intracellularly and lack an extracellular phase. Here we determined the cryo-EM structure at 3.7 Å resolution of Rosellinia necatrix quadrivirus 1 (RnQV1), a fungal double-stranded (ds)RNA virus. RnQV1, the type species of the family Quadriviridae, has a multipartite genome consisting of four monocistronic segments. Whereas most dsRNA virus capsids are based on dimers of a single protein, the ~450-Å-diameter, T = 1 RnQV1 capsid is built of P2 and P4 protein heterodimers, each with more than 1000 residues. Despite a lack of sequence similarity between …