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Full-Text Articles in Organisms
Herbicide Mediated Selection Of Sulfonamide Resistant E. Coli, Laura P. Muntz
Herbicide Mediated Selection Of Sulfonamide Resistant E. Coli, Laura P. Muntz
Electronic Thesis and Dissertation Repository
Asulam is an herbicide that is structurally analogous to sulfonamide antibiotics (sulfas), with the potential to contribute to the global antibiotic resistance crisis by cross-selecting for sulfa-resistant bacteria. To determine if asulam can select for antibiotic resistant bacteria, isothermal titration calorimetry (ITC) was used to assess the binding affinity of asulam with the target protein of sulfas, dihydropteroate synthase (DHPS). ITC confirmed asulam interacts with DHPS, and in vitro directed evolution experiments showed that prolonged asulam exposure can select for sulfa-insensitive DHPS in E. coli. Since mobile sul genes also encode for sulfa-insensitive DHPS, the potential effect of asulam on …
Bacteriophages: Paving The Road For The Future Of Medicine, Luke Brinkerhoff
Bacteriophages: Paving The Road For The Future Of Medicine, Luke Brinkerhoff
Honors Theses
Bacteriophages are a possible solution to antibiotic resistance, which is predicted to be detrimental world-wide by the year 2050. Personal field research was also conducted for a project studying the characteristics of two bacteriophages on a single bacterial host.
Diversity Of Bacteriophage In Burkholderia Species, Abigail Price
Diversity Of Bacteriophage In Burkholderia Species, Abigail Price
Honors Projects
Bacteriophages are viruses that infect and replicate within bacteria and offer the potential of a therapeutic alternative to chronic infections that do not respond to antibiotic-based therapies. B. vietnamiensis is one of a number of Burkholderia species involved with chronic drug resistant infections in the lungs of individuals with compromised respiratory systems, as found in those with chronic obstructive pulmonary disease and, most especially, are of particular significance in patients with cystic fibrosis. The diversity of the Burkholderia species is explored by using online databases and looking at bacteriophage or phage-encoding viruses found in B. vietnamiensis. The open reading frames …
The Role Of Coagulase-Negative Staphylococcal Secreted Products On Staphylococcus Aureus And Staphylococcus Lugdunensis Infections, Denny Chin
Electronic Thesis and Dissertation Repository
The Staphylococcus genus is comprised of over 40 bacterial species. The most well-studied species in this genus is the notorious human pathogen Staphylococcus aureus, a bacterium that produces coagulase among many other virulence factors. Since S. aureus is a major health burden and causes a plethora of diseases in humans, it has received significant attention and much research has been done to understand its biology to treat diseases caused by this pathogen. However, the coagulase-negative staphylococci (CoNS) make up most of the staphylococcal species and have received less attention since they are thought to have a lesser impact on …