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Full-Text Articles in Organisms

Crispr/Cas9 In Yeast: A Multi-Week Laboratory Exercise For Undergraduate Students, Randi J. Ulbricht May 2019

Crispr/Cas9 In Yeast: A Multi-Week Laboratory Exercise For Undergraduate Students, Randi J. Ulbricht

Open Educational Resources

Providing undergraduate life-science students with a course-based research experience that utilizes cutting-edge technology, is tractable for students, and is manageable as an instructor is a challenge. Here, I describe a multi-week lesson plan for a laboratory-based course with the goal of editing the genome of budding yeast, Saccharomyces cerevisiae. Students apply knowledge regarding advanced topics such as: CRISPR/Cas9 gene editing, DNA repair, genetics, and cloning. The lesson requires students to master skills such as bioinformatics analysis, restriction enzyme digestion, ligation, basic microbiology skills, polymerase chain reaction, and plasmid purification. Instructors are led through the technical aspects of the protocols, …


Estudios En Biodiversidad, Volumen I, Griselda Pulido-Flores, Scott Monks, Maritza López-Herrera Nov 2015

Estudios En Biodiversidad, Volumen I, Griselda Pulido-Flores, Scott Monks, Maritza López-Herrera

Zea E-Books Collection

Este libro es el producto del trabajo colegiado que han desarrollado los profesores, investigadores, y estudiantes que conforman la Red Temática de Calidad Ambiental y Desarrollo Sustentable, a través del macroproyecto “Evaluación del impacto ambiental por actividades antropogénicas: Alternativas de mitigación”. El cual fue apoyado con recursos financieros por parte del Programa para el Desarrollo Profesional Docente (PRODEP), de la Secretaria de Educación Pública (SEP) en México. Las instituciones de educación superior y cuerpos participantes en la red temática son la Universidad Autónoma del Estado de Hidalgo (Uso, Manejo y Conservación de la Biodiversidad UAEHCA- 10; Ciencias Ambientales UAEH-CA-59); Universidad …