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Full-Text Articles in Organisms
Comparison Of Wild And Cultivated Extracts Of Cordyceps Sinensis Apoptotic Potential, Katelyn Staring
Comparison Of Wild And Cultivated Extracts Of Cordyceps Sinensis Apoptotic Potential, Katelyn Staring
Honors Theses
Cordyceps sinensis is a mushroom which contains the compound cordycepin (3’-deoxyadenosine), an analogue of adenosine. In Traditional Chinese Medicine (TCM), cordycepin has multipurpose pharmacological uses including purported anti-tumor effects. In the present study, cordycepin was extracted from the wild mushroom as well as from various commercially available cultivated extracts. Previous research in this lab has demonstrated that cultivated extracts contain less cordycepin than the wild mushroom. However, it is unclear if the decrease in cordycepin correlates with decreased activity. To measure anti-tumor activity, extracts were used to treat human breast cancer cells (MCF-7 cells). In other labs, cordycepin has been …
Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Honors Theses
The overall goal of this project was to investigate the biological role of a putative metacaspase gene present in the mushroom fungus Schizophyllum commune. For this study, we have utilized a strain of S. commune that is unable to integrate DNA via the non-homologous end joining pathway. This forces transforming DNA to integrate homologously, as is required for the purposes of gene knockout. The gene Scp1 encodes a likely member of the metacaspase protein family, which are suspected to have activity similar to caspases, the latter crucial to programmed cell death. A knockout construct containing a non-functional version of Scp1 …