Organisms Commons^™

Revival Of Metabolism In Rehydrated Marasmius Oreades, Lorie J. Warren

Honors Theses, 1963-2015

Mushrooms from the genus Marasmius have the ability to revive -- viz., dried specimens regain their original shape when moistened. The purpose of this study was to determine if the basidiocarps are metabolically active upon revival or whether they simply absorb moisture like a sponge. The reduction of tetrazolium chloride to a red, water-insoluble formazan pigment was used as a measure of respiratory activity. Basidiocarp samples were incubated in a phosphate buffer containing 1% tetrasolium chloride. After a suitable period, the samples were extracted with MeOH to remove any formazan pigment and the absorbance measured at 530 nm. Fresh M. …