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Full-Text Articles in Organisms
The Pam-1 Aminopeptidase Protects Against Neurodegeneration In Caenorhabditis Elegans, Caleb Coil
The Pam-1 Aminopeptidase Protects Against Neurodegeneration In Caenorhabditis Elegans, Caleb Coil
Honors College Theses
The deteriorating effects of neurodegenerative conditions seem inescapable for many as they age. The experimental exploration of the molecular and cellular mechanisms responsible for neurodegeneration is aided using animal models. For instance, genetic screens have identified the puromycin-sensitive aminopeptidase (PSA) as a novel effector of neurodegeneration, as mutations in PSA in fruit flies result in perturbations of neuron anatomy and an accelerated onset of neuron death. We have tested the hypothesis that PAM-1, the Caenorhabditis elegans ortholog of PSA, plays a similar role in governing neurodegeneration in nematodes. Genetic strains were created that express the green fluorescent protein (GFP) in …
Site Tolerance Of The Rada Intein, Chase Taul, Christopher Lennon Dr
Site Tolerance Of The Rada Intein, Chase Taul, Christopher Lennon Dr
Honors College Theses
Inteins (intervening proteins) invade genes at the DNA level and splice out at the protein level. Once thought of as only a parasitic type of a mobile genetic element, recent work suggests a mutualistic relationship has formed in some cases within bacterial and archaeal hosts. After translation, a precursor protein is formed with the intein between two exteins. The intein is catalytic and can excise itself out through protein splicing. Intein insertion is biased towards the active site of the protein and is thought to cause inactivation of the host protein prior to splicing. Intein splicing is responsive to a …