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Full-Text Articles in Medical Specialties
Study Of Polytopic Membrane Protein Topological Organization As A Function Of Membrane Lipid Composition, Mikhail Bogdanov, Philip N Heacock, William Dowhan
Study Of Polytopic Membrane Protein Topological Organization As A Function Of Membrane Lipid Composition, Mikhail Bogdanov, Philip N Heacock, William Dowhan
Journal Articles
A protocol is described using lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by the substituted-cysteine accessibility method as applied to transmembrane domains (SCAM). SCAM is adapted to follow changes in membrane protein topology as a function of changes in membrane lipid composition. The strategy described can be adapted to any membrane system.