Other Medical Sciences Commons^™

Dynamic Mechanostimulation Of Live Cells During Real-Time Microscopy, Daniel Lorusso

Electronic Thesis and Dissertation Repository

Our body’s functioning depends on the ability of cells to sense and react to their local mechanical environment; this process is known as mechanotransduction. Despite the importance of understanding how cells interact with mechanical stimuli, the specific mechanisms governing such processes have yet to be elucidated. Using microscopy to detect the early responses of living cells to mechanical loads and forces would be a critical step towards further understanding cellular mechanotransduction. Dynamic and high-frequency cyclical loads are relevant to human physiology and disease. Yet, modern microscopy systems are not capable of delivering the appropriate mechanical stimuli to live cell cultures. …