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Full-Text Articles in Medical Sciences
The Role Of Cd4+T Cells In The Immune Response To Trypanosoma Cruzi In Mice Held At Elevated Environmental Temperature, Qi Guo
Masters Theses & Specialist Projects
The immune response of mice infected with Trypanosoma cruzi (the causative agent of Chagas' disease) has been extensively studied, and it has been demonstrated that changes in environmental temperature can influence the resistance of infected mice to the parasite. In particular, several investigators have demonstrated an increased resistance to T. cruzi infection when infected mice are maintained at 36°C. This increased resistance is reflected in decreased parasitemias and increased longevity. In addition, this enhanced resistance has been shown to be an immunological phenomenon. CD4+ T cells play a very important role in immune responses to parasitic infections through the secretion …
Comparison Of Micrscan Identification And Susceptibility Testing Methods For Streptococcus Dysgalactiae To Conventional Biochemical Reactions And Kirby-Bauer Susceptibility Testing Methods, Anthony Hatcher
Masters Theses & Specialist Projects
A total of 100 isolates of Streptococcus dysgalact iae from bovine mastitis infections was used to evaluate and compare the biochemical reactions of the gram positive Combo Type 6 panel on the MicroScan autoSCANR system to a conventional procedure for identification of streptococci. Of the 100 isolates, 83.3% was identified as "Very Rare Biotype" by the MicroScan and classified as S. dysgalactiae by conventional methods. Of the remaining 16.7%, MicroScan identified 3.3% as Streptococcus morbillorum, 3.3% as Aerococcus viridians, 3.3% as Streptococcus constellatum/milleri, 1.7% as Streptococcus agalactiae, 1.7% as Streptococcus mitis, 1.7% as Streptococcus sanguis, and 1.7% as Streptococcus intermidis/rni …
The Role Of Cd8+T Cells In The Immune Response To Trypanosoma Cruzi In Mice Held At Elevated Environmental Temperature, Zhijan Ming
Masters Theses & Specialist Projects
When maintained at 36°C, T. cruzi infected C3H mice survive an infection that is lethal to mice maintained at room temperature. To study the role of CD8+ T cells in this phenomenon, anti-CD8 monoclonal antibody (MAb) was used to deplete CD8+ T cells in vivo. An IgG2a-producing rat hybridoma (designated 53-6.72) was adapted to serum free medium, and anti-CD8 MAb was purified from culture supernatant by ammonium sulfate precipitation. Mice were injected intraperitoneally with 200(ig of MAb for three consecutive days. On the fifth day, the result of the in vivo depletion of CD8+ T cells was determined by an …
Evaluation And Comparison Of Environmental Contaminants Present In Two Types Of Swine Rearing Facilities, Carolyn Crowe
Evaluation And Comparison Of Environmental Contaminants Present In Two Types Of Swine Rearing Facilities, Carolyn Crowe
Masters Theses & Specialist Projects
It has been reported that pigs weaned and moved into an isolated nursery have greater growth rates measured on an average daily gain, and thymus gland weights than do their counterparts in a conventional nursery. In this study I explored the association of this biological difference with environmental contaminants. Levels of total and respirable dust and endotoxin, carbon monoxide, carbon dioxide, ammonia, and temperature and percent relative humidity were measured in two types of swine rearing facilities: isolated nursery and nursery within a conventional farm. We found isolated nurseries have significantly (p<0.05) less total and respirable dust and endotoxin and greater growth rates. With few exceptions, our measurements of levels of total and respirable dust and total endotoxin were within published ranges. Levels of respirable endotoxin were higher than published ranges.
Optimizing Conditions For Cloning Cdna From Chicken Brain, Stephen Clark
Optimizing Conditions For Cloning Cdna From Chicken Brain, Stephen Clark
Masters Theses & Specialist Projects
Acoustic over stimulation can lead to hair cell loss in the auditory epithelium. Hair cell loss or damage in mammals often results in decreased auditory sensitivity which is irreversible. However for birds, hair cells which are damaged by acoustic trauma or ototoxic drugs may be replaced by regeneration. As the first step in determining the molecular mechanisms of hair cell regeneration, gene expression techniques including cDNA synthesis and cloning were attempted first with the chicken brain. The initial attempts at making cDNA libraries were elusive; therefore, experiments were performed to identify problems and enhance the efficiency of specific steps. These …