Medical Sciences Commons^™

A Role For Regulator Of G Protein Signaling-12 (Rgs12) In The Balance Between Myoblast Proliferation And Differentiation, Adam B. Schroer, Junaith S. Mohamed, Melinda D. Wilard, Vincent Setola, Emily Oesteich, David P. Siderovski

Faculty & Staff Scholarship

• Regulators of G Protein Signaling (RGS proteins) inhibit G protein-coupled receptor (GPCR) signaling by accelerating the GTP hydrolysis rate of activated Gα subunits. Some RGS proteins exert additional signal modulatory functions, and RGS12 is one such protein, with five additional, functional domains: a PDZ domain, a phosphotyrosine-binding domain, two Ras-binding domains, and a Gα·GDP-binding GoLoco motif. RGS12 expression is temporospatially regulated in developing mouse embryos, with notable expression in somites and developing skeletal muscle. We therefore examined whether RGS12 is involved in the skeletal muscle myogenic program. In the adult mouse, RGS12 is expressed in the tibialis anterior (TA) muscle, …

H2o2‐Induced Microvessel Barrier Dysfunction: The Interplay Between Reactive Oxygen Species, Nitric Oxide, And Peroxynitrite, Xueping Zhou, Yan Qian, Dong Yuan, Qilong Feng, Pingnian He

Faculty & Staff Scholarship

Elevated H2O2 is implicated in many cardiovascular diseases. We previously demonstrated that H2O2-induced endothelial nitric oxide synthase (eNOS) activation and excessive NO production contribute to vascular cell injury and increases in microvessel permeability. However, the mechanisms of excessive NO-mediated vascular injury and hyperpermeability remain unknown. This study aims to examine the functional role of NO-derived peroxynitrite (ONOO) in H2O2-induced vascular barrier dysfunction by elucidating the interrelationships between H2O2-induced NO, superoxide, ONOO, and changes in endothelial [Ca2+ ]i and microvessel permeability. Experiments were conducted on intact rat mesenteric venules. Microvessel permeability was determined by measuring hydraulic conductivity (Lp). Endothelial [Ca2+ ]i, …