Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Medical Sciences
Evaluation Of [14c] And [13c]Sucrose As Blood-Brain Barrier Permeability Markers, Mohammad K. Miah, Ekram A. Chowdhury, Ulrich Bickel, Reza Mehvar
Evaluation Of [14c] And [13c]Sucrose As Blood-Brain Barrier Permeability Markers, Mohammad K. Miah, Ekram A. Chowdhury, Ulrich Bickel, Reza Mehvar
Pharmacy Faculty Articles and Research
Non-specific quantitation of [14C]sucrose in blood and brain has been routinely used as a quantitative measure of the in vivo blood-brain barrier (BBB) integrity. However, the reported apparent brain uptake clearance (Kin) of the marker varies widely (∼100 fold). We investigated the accuracy of the use of the marker in comparison with a stable isotope of sucrose ([13C]sucrose) measured by a specific LC-MS/MS method. Rats received single doses of each marker, and the Kin values were determined. Surprisingly, the Kin value of [13C]sucrose was 6-7 fold lower than that …