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Il-4 Engagement Of The Type 1 Il-4 Receptor Complex Enhances Mouse Eosinophil Migration To Eotaxin-1 In Vitro, Nicola M. Heller, William M. Gwinn, Raymond P. Donnelly, Stephanie L. Constant, Achsah D. Keegan
Il-4 Engagement Of The Type 1 Il-4 Receptor Complex Enhances Mouse Eosinophil Migration To Eotaxin-1 In Vitro, Nicola M. Heller, William M. Gwinn, Raymond P. Donnelly, Stephanie L. Constant, Achsah D. Keegan
Microbiology, Immunology, and Tropical Medicine Faculty Publications
Background
Previous work from our laboratory demonstrated that IL-4Rα expression on a myeloid cell type was responsible for enhancement of Th2-driven eosinophilic inflammation in a mouse model of allergic lung inflammation. Subsequently, we have shown that IL-4 signaling through type I IL-4 receptors on monocytes/macrophages strongly induced activation of the IRS-2 pathway and a subset of genes characteristic of alternatively activated macrophages. The direct effect(s) of IL-4 and IL-13 on mouse eosinophils are not clear. The goal of this study was determine the effect of IL-4 and IL-13 on mouse eosinophil function.
Methods
Standard Transwell chemotaxis assay was used to …