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Department of Cancer Biology Faculty Papers
Animal experiment; antibody detection; antibody production; antibody response; antigen antibody reaction; antigen binding; antigen specificity; article; controlled study; exposure; fucosylation; glycosylation; immunoassay; immunoreactivity; immunostimulation; limit of detection; mass spectrometry; microarray analysis; mouse; nonhuman; nucleotide sequence; rabbit; serology; aleuria aurantia lectin; Freund adjuvant; immunoglobulin Fc fragment; immunoglobulin G antibody; lectin; ovalbumin; unclassified drug
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Full-Text Articles in Medical Sciences
Aleuria Aurantia Lectin (Aal)-Reactive Immunoglobulin G Rapidly Appears In Sera Of Animals Following Antigen Exposure., Songming Chen, Chen Lu, Hongbo Gu, Anand Mehta, Jianwei Li, Patrick B Romano, David Horn, D Craig Hooper, Carthene R Bazemore-Walker, Timothy Block
Aleuria Aurantia Lectin (Aal)-Reactive Immunoglobulin G Rapidly Appears In Sera Of Animals Following Antigen Exposure., Songming Chen, Chen Lu, Hongbo Gu, Anand Mehta, Jianwei Li, Patrick B Romano, David Horn, D Craig Hooper, Carthene R Bazemore-Walker, Timothy Block
Department of Cancer Biology Faculty Papers
We have discovered an Aleuria Aurantia Lectin (AAL)-reactive immunoglobulin G (IgG) that naturally occurs in the circulation of rabbits and mice, following immune responses induced by various foreign antigens. AAL can specifically bind to fucose moieties on glycoproteins. However, most serum IgGs are poorly bound by AAL unless they are denatured or treated with glycosidase. In this study, using an immunogen-independent AAL-antibody microarray assay that we developed, we detected AAL-reactive IgG in the sera of all animals that had been immunized 1-2 weeks previously with various immunogens with and without adjuvants and developed immunogen-specific responses. All of these animals subsequently …