Medical Sciences Commons^™

Advantage Of Precision Metagenomics For Urinary Tract Infection Diagnostics, Sadia Almas, Rob E. Carpenter, Chase Rowan, Vaibhav Tamrakar, Rahul Sharma

Human Resource Development Faculty Publications and Presentations

Background: Urinary tract infections (UTIs) remain a diagnostic challenge and often promote antibiotic overuse. Despite urine culture being the gold standard for UTI diagnosis, some uropathogens may lead to false-negative or inconclusive results. Although PCR testing is fast and highly sensitive, its diagnostic yield is limited to targeted microorganisms. Metagenomic next-generation sequencing (mNGS) is a hypothesis-free approach with potential of deciphering the urobiome. However, clinically relevant information is often buried in the enormous amount of sequencing data.

Methods: Precision metagenomics (PM) is a hybridization capture-based method with potential of enhanced discovery power and better diagnostic yield without diluting clinically relevant …

Sars-Cov-2 Next Generation Sequencing (Ngs) Data From Clinical Isolates From The East Texas Region Of The United States, Rob E. Carpenter, Vaibhav K. Tamrakar, Advanta Genetics

Human Resource Development Faculty Publications and Presentations

The SARS-CoV-2 virus has evolved throughout the pandemic and is likely to continue evolving into new variants. Some of these variants may affect functional properties, including infectivity, interactions with host immunity, and disease severity. And compromised vaccine efficacy is an emerging concern with every new viral variant. Next-generation sequencing (NGS) has emerged as the tool of choice for discovering new variants and understanding the transmission dynamics of SARS-CoV-2. Deciphering the SARS-CoV-2 genome has enabled epidemiological survivance and forecast of altered etiologically. Clinical presentations of the infection are influenced by comorbidities such as age, immune status, diabetes, and the infecting variant. …