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U2os Cells Lacking Chk1 Undergo Aberrant Mitosis And Fail To Activate The Spindle Checkpoint, Laura Carrassa, Yolanda Sanchez, Eugenio Erba, Giovanna Damia
U2os Cells Lacking Chk1 Undergo Aberrant Mitosis And Fail To Activate The Spindle Checkpoint, Laura Carrassa, Yolanda Sanchez, Eugenio Erba, Giovanna Damia
Dartmouth Scholarship
Chk1 is a conserved protein kinase originally identified in fission yeast, required to delay entry of cells with damaged or unreplicated DNA into mitosis. The requirement of Chk1 for both S and G2/M checkpoints has been elucidated while only few studies have connected Chk1 to the mitotic spindle checkpoint. We used a small interference RNA strategy to investigate the role of Chk1 in unstressed conditions. Chk1 depletion in U2OS human osteosarcoma cells inhibited cell proliferation and raised the percentage of cells with a 4N DNA content, which correlated with accumulation of giant polynucleated cells morphologically distinct from apoptotic cells, while …