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Full-Text Articles in Medical Sciences
Ouabain Decreases Sarco(Endo)Plasmic Reticulum Calcium Atpase Activity In Rat Hearts By A Process Involving Protein Oxidation, David Kennedy, Sandeep Vetteth, Miaorong Xie, Sankaridrug Periyasamy, Jingwei Xie, Chi Han, Venkatesha Basrur, Krishna Mutgi, Vladimir Fedorov, Deepak Malhotra, Joseph Shapiro
Ouabain Decreases Sarco(Endo)Plasmic Reticulum Calcium Atpase Activity In Rat Hearts By A Process Involving Protein Oxidation, David Kennedy, Sandeep Vetteth, Miaorong Xie, Sankaridrug Periyasamy, Jingwei Xie, Chi Han, Venkatesha Basrur, Krishna Mutgi, Vladimir Fedorov, Deepak Malhotra, Joseph Shapiro
Jingwei Xie
The effect of cardiac glycosides to increase cardiac inotropy by altering Ca2+ cycling is well known but still poorly understood. The studies described in this report focus on defining the effects of ouabain signaling on sarcoplasmic reticulum Ca2+-ATPase function. Rat cardiac myocytes treated with 50 μM ouabain demonstrated substantial increases in systolic and diastolic Ca2+ concentrations. The recovery time constant for the Ca2+ transient, τ, was significantly prolonged by ouabain. Exposure to 10 μM H2O2, which causes an increase in intracellular reactive oxygen species similar to that of 50 μM ouabain, caused a similar increase in τ. Concurrent exposure to …
Effects Of Cardiotonic Steroids On Dermal Collagen Synthesis And Wound Healing, Nasser El-Okdi, Sleiman Smaili, Vanamala Raju, Amjad Shidyak, Shalini Gupta, Larisa Fedorova, Jihad Elkareh, Sankaridrug Periyasamy, Anna Shapiro, M. Kahaleh, Deepak Malhotra, Jingwei Xie, Khew Chin, Joseph Shapiro
Effects Of Cardiotonic Steroids On Dermal Collagen Synthesis And Wound Healing, Nasser El-Okdi, Sleiman Smaili, Vanamala Raju, Amjad Shidyak, Shalini Gupta, Larisa Fedorova, Jihad Elkareh, Sankaridrug Periyasamy, Anna Shapiro, M. Kahaleh, Deepak Malhotra, Jingwei Xie, Khew Chin, Joseph Shapiro
Jingwei Xie
We previously reported that cardiotonic steroids stimulate collagen synthesis by cardiac fibroblasts in a process that involves signaling through the Na-K-ATPase pathway (Elkareh et al. Hypertension 49: 215–224, 2007). In this study, we examined the effect of cardiotonic steroids on dermal fibroblasts collagen synthesis and on wound healing. Increased collagen expression by human dermal fibroblasts was noted in response to the cardiotonic steroid marinobufagenin in a dose- and time-dependent fashion. An eightfold increase in collagen synthesis was noted when cells were exposed to 10 nM marinobufagenin for 24 h (P < 0.01). Similar increases in proline incorporation were seen following treatment with digoxin, ouabain, and marinobufagenin (10 nM × 24 h, all results P < 0.01 vs. control). The coadministration of the Src inhibitor PP2 or N-acetylcysteine completely prevented collagen stimulation by marinobufagenin. …