Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Diseases
Developing Zntpp-4ab As A Potential Photodynamic Therapy Agent, Kennedy Johnson
Developing Zntpp-4ab As A Potential Photodynamic Therapy Agent, Kennedy Johnson
Scholars Day Conference
Photodynamic therapy is a fairly new technique used in cancer treatment involving the use of a photosensitizer and light exposure to kill malignant cells. In this research, the use of ZnTPP-4AB as a photosensitizer and red light as a source of radiant light energy was explored in terms of cytotoxicity in the A549 lung cancer cell line. ZnTPP-4AB was synthesized by replacing the carbonyl functional groups on the ZnTPPC porphyrin with 4-amino-1-butanol. Following synthesis, the new porphyrin was purified through the use of Sephadex LH-20 and G-50 in column chromatography in order to remove impurities. To confirm the identity and …
Analyzing Wisp Dna Sequences And Building Primers For Dna Separation, Paige Spicer, Payton Crum
Analyzing Wisp Dna Sequences And Building Primers For Dna Separation, Paige Spicer, Payton Crum
Scholars Day Conference
A bacteriophage is a virus that infects bacteria. Bacteriophage DNA was isolated from a lysate sample, but two separate, unrelated genome sequences were returned. Plaque assays and plaque streaking were used to in an effort to separate the phages. After plaque isolation was unsuccessful, NCBI BLAST and IDT software were used to build unique primers for each of the two unrelated genomes. After receiving the primers, PCR and gel electrophoresis were run on plaques and lysate from each phage to classify each phage in the correct cluster and sub cluster as identified by the original DNA sequencing.
Progress Towards Phage Stability For Possible Oral Phage Therapy, Erin Russo
Progress Towards Phage Stability For Possible Oral Phage Therapy, Erin Russo
Scholars Day Conference
Oral cavities or caries is a localized deterioration of the tooth caused by an accumulation of harmful bacteria. This research intends to integrate bacteriophages onto unwaxed dental floss as a possible future preventative treatment for oral cavities. Bacteriophages are viruses that degrade the composition of bacterial populations by invading and reproducing inside bacterial hosts. The experimental framework involves soaking unwaxed floss in the bacteriophages named Phrick and Stonehill. The effectiveness of infection is measured by applying the phage-infused floss on an agar plate with Gordonia terrae CAG3, then analyzing the diameter of the plaque around the floss. This research will …