Diseases Commons^™

The Development Of Novel Imaging Modalities & High-Throughput Drug Screening Platforms In The Drosophila Melanogaster Model Of Human Calcium Oxalate Nephrolithiasis, Sohrab Naushad Ali

Electronic Thesis and Dissertation Repository

Nephrolithiasis is a common urological disorder. Despite advances in the surgical treatment of kidney stone disease, research into its prevention and medical management remain stagnant. This is due to lack of viable pre-clinical models to study the disorder. In this project, we develop and characterize a robust Drosophila melanogaster model for human calcium oxalate nephrolithiasis. Using this model, we have developed intravital imaging techniques to study stone formation and novel high-throughput drug screening platforms. We successfully demonstrate calcium oxalate stone formation by sodium oxalate and ethylene glycol supplementation, with subsequent intravital imaging using bisphosphonate based fluorescent probes. Screening of 360 …