Open Access. Powered by Scholars. Published by Universities.®
Orthodontics and Orthodontology Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Orthodontics and Orthodontology
Reprogramming Oral Epithelial Keratinocytes Into A Pluripotent Phenotype For Tissue Regeneration, Fayrouz Bazina, Sabine M. Brouxhon, Stephanos Kyrkanides
Reprogramming Oral Epithelial Keratinocytes Into A Pluripotent Phenotype For Tissue Regeneration, Fayrouz Bazina, Sabine M. Brouxhon, Stephanos Kyrkanides
Center for Oral Health Research Faculty Publications
OBJECTIVES: We set out to reprogram adult somatic oral epithelial keratinocytes into pluripotent cells for regenerative dentistry.
SETTING AND SAMPLE POPULATION: Immortalized murine oral keratinocyte cell (IMOK) line raised from adult mouse mucosa were cultured in vitro in our studies.
MATERIALS AND METHODS: Adult murine oral epithelial keratinocytes were chronically treated with TGF-β1 in vitro, and the expression of Oct4, Nanog, Sox2 and Nestin, as well as specific homeobox Gata and Pax gene family members were investigated.
RESULTS: We documented the induction of stem factors linked with pluripotency and/or the maintenance and regulation of stem-cell self-renewal in oral epithelial keratinocytes …