Oral Biology and Oral Pathology Commons^™

Detection Of The Argonaute Protein Ago2 And Micrornas In The Rna Induced Silencing Complex (Risc) Using A Monoclonal Antibody, K. Ikeda, M. Satoh, Kaleb M. Pauley, M. J. Fritzler, W. H. Reeves, E. K. Chan

Science and Mathematics Faculty Publications

MicroRNAs (miRNAs) are short RNA molecules responsible for post-transcriptional gene silencing by the degradation or translational inhibition of their target messenger RNAs (mRNAs). This process of gene silencing, known as RNA interference (RNAi), is mediated by highly conserved Argonaute (Ago) proteins which are the key components of the RNA induced silencing complex (RISC). In humans, Ago2 is responsible for the endonuclease cleavage of targeted mRNA and it interacts with the mRNAbinding protein GW182, which is a marker for cytoplasmic foci referred to as GW bodies (GWBs). We demonstrated that the antiAgo2 monoclonal antibody 4F9 recognized GWBs in a cell cycle …