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Articles 481 - 510 of 599
Full-Text Articles in Dentistry
Ultrasound-Guided Vs Conventional Intraarticular Injection Of Hyaluronic Acid For Management Of Temporomandibular Joint Internal Derangement: A Randomized Controlled Trial, Muhanad Almufarrih, Muhammad Al-Hawari, Ayman Yaseen, Sally Abdelsameaa
Ultrasound-Guided Vs Conventional Intraarticular Injection Of Hyaluronic Acid For Management Of Temporomandibular Joint Internal Derangement: A Randomized Controlled Trial, Muhanad Almufarrih, Muhammad Al-Hawari, Ayman Yaseen, Sally Abdelsameaa
Mansoura Journal of Dentistry
Objective: This study aimed to evaluate the clinical efficacy of using ultrasound guidance during intra-articular injection of hyaluronic acid (HA) in the management of patients with TMJ internal derangement. Material/subjects and Methods: Forty patients complaining of anterior disc displacement with reduction (DDwR) not responsive to conservative treatment were enrolled in this study. The diagnosis was based on the current update of diagnostic criteria for temporomandibular disorders (TMDs). Intra-articular injection of Hyaluronic Acid guided by Ultrasound in the first group and Intra-articular Injection (IAI) of HA conventional way in the second group were performed. Articular pain was evaluated using the visual …
Effect Of Presence Of Cervical Composite Restoration On Intraoral Scanner Precision Of Anterior Laminate Veneer Preparations – An In Vitro Study, Mohamed Tarek Shanshan, Walid Abd El-Ghafar Al-Zordok, Mohamed Hamed Ghazy
Effect Of Presence Of Cervical Composite Restoration On Intraoral Scanner Precision Of Anterior Laminate Veneer Preparations – An In Vitro Study, Mohamed Tarek Shanshan, Walid Abd El-Ghafar Al-Zordok, Mohamed Hamed Ghazy
Mansoura Journal of Dentistry
Objective
To evaluate the effect of cervical composite restoration on the precision of digital impressions in anterior laminate veneer preparations.
Materials and methods
Forty-eight sound human maxillary central incisors were collected and prepared for anterior laminate veneer restorations following standardized preparation protocol. Specimens were grouped into two equal groups according to the presence of cervical composite restoration: With cervical restoration and without cervical restoration. All preparations were scanned 10 times repeatedly using Primescan intraoral scanner and exported as standard tessellation language (STL) files. Precision was evaluated by calculating root mean square (RMS) values through three-dimensional superimposition analysis using Geomagic Control …
Fracture Resistance Of Monolithic Zirconia Restorations After Endodontic Access Cavity Preparation: Effect Of Repair Method, Isra Osman Mohammed, Esaama. Khedr, Lamia E. Dawood, Walid Abd Elghafar Al-Zordok
Fracture Resistance Of Monolithic Zirconia Restorations After Endodontic Access Cavity Preparation: Effect Of Repair Method, Isra Osman Mohammed, Esaama. Khedr, Lamia E. Dawood, Walid Abd Elghafar Al-Zordok
Mansoura Journal of Dentistry
objective: The present study was performed to assess the effect of restoration of access cavity with composite resin filling on fracture resistance of multilayered zirconia crowns.
Materials and Methods:
Thirty-three sound human mandibular first molars were prepared and restored with three types of monolithic zirconia crowns: High-Translucent Multilayered Plus (HTML), Yttria Multilayered (YML), and Ultra-Translucent Multilayered (UTML) (N=11 each). Standardized access cavities were prepared through the occlusal surfaces, endodontic treatment was completed, and each access opening was repaired using incremental composite resin. The specimens were subjected to thermocycling (10,000 cycles between 5 °C and 55 °C) and cyclic mechanical loading …
Extended Platelet Rich Fibrin In Lateral Maxillary Sinus Lifting With Simultaneous Implant Placement, Ammar Radwan Samman, Heba Abo-Elfetouh Elsheikh, Walid Abd El-Ghafar Al-Zordk, Noha Ahmed Mansour
Extended Platelet Rich Fibrin In Lateral Maxillary Sinus Lifting With Simultaneous Implant Placement, Ammar Radwan Samman, Heba Abo-Elfetouh Elsheikh, Walid Abd El-Ghafar Al-Zordk, Noha Ahmed Mansour
Mansoura Journal of Dentistry
Objective: This study aimed to investigate the influence of extended platelet-rich fibrin (e-PRF) when used in lateral maxillary sinus floor augmentation (MSFA), with particular emphasis on implant stability and peri-implant marginal bone loss.
Materials and Methods: Thirteen patients presenting with posterior maxillary tooth loss and a residual bone height of 3–5 mm underwent lateral sinus floor augmentation with concomitant implant placement. A total of 16 implant sites were included and equally allocated into two groups: Group I (n = 8), in which an allograft was applied and covered using a pericardium membrane, and Group II (n = 8), in which …
Effect Of Biodentine And Treated Dentine Matrix On Viability And Proliferation Of Human Dental Pulp Stem Cells, Nada Barakat, Rana El-Qashty, Mohamad E. Helal
Effect Of Biodentine And Treated Dentine Matrix On Viability And Proliferation Of Human Dental Pulp Stem Cells, Nada Barakat, Rana El-Qashty, Mohamad E. Helal
Mansoura Journal of Dentistry
Objective: The present study aimed to assess the dose-dependent effect of Biodentine (BD) and human-treated dentin matrix (hTDM) on the proliferation of human dental pulp stem cells (hDPSCs).
Materials and methods: hTDM was prepared and characterized. The concentration of proteins released from BD and hTDM extracts were quantified using the bicinchoninic acid (BCA) assay after 3 and 7 days. hDPSCs were successfully isolated and characterized, then treated with various concentrations of BD extracts (20, 2, and 0.2 mg/mL) and hTDM extracts (100%, 80%, 60%, 40%, 20%, and 10%). Cell viability was determined after 7 days of incubation using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium …
Survival Rate And Prognostic Criteria In Oral Squamous Cell Carcinoma Patients: A Retrospective Analysis, Shihab Ahmed Jassim, Shadi Awny Hasan, Mohamed Mahmoud Anees, Jilan Mohamed Youssef
Survival Rate And Prognostic Criteria In Oral Squamous Cell Carcinoma Patients: A Retrospective Analysis, Shihab Ahmed Jassim, Shadi Awny Hasan, Mohamed Mahmoud Anees, Jilan Mohamed Youssef
Mansoura Journal of Dentistry
Objective:
To evaluate overall survival, disease-free survival (DFS), and associated prognostic factors among patients with oral squamous cell carcinoma (OSCC) treated at a tertiary oncology center in Egypt.
Materials/Subjects and Methods: This retrospective cohort study was conducted at the Surgical Oncology Department, Oncology Center, Mansoura University, Egypt. Medical records of patients with histopathologically confirmed primary OSCC diagnosed between January 2011 and December 2021 were reviewed. A total of 140 eligible patients were included. Data collected included demographic characteristics, selected medical history, tumor site, tumor size, TNM stage, histological grade, treatment modality, and follow-up status. Data were entered, managed, and analyzed …
An Attempt For In Situ Bio-Root Regeneration By Using Treated Dentin Matrix And Dental Pulp Stem Cells In Albino Rats, Amani Mohamad, Ahmad A. Emam, Eman Hany, Mohamed Abdulrahman
An Attempt For In Situ Bio-Root Regeneration By Using Treated Dentin Matrix And Dental Pulp Stem Cells In Albino Rats, Amani Mohamad, Ahmad A. Emam, Eman Hany, Mohamed Abdulrahman
Mansoura Journal of Dentistry
Objectives: This study was conducted to prepare and characterize root-shaped treated dentin matrix (TDM) scaffolds, and to evaluate their efficacy together with seeded dental pulp stem cells (DPSCs) as potential bio-roots in extraction sockets of rat models.
Materials and Methods: Root-shaped TDM specimens were prepared and characterized. Following extraction of maxillary incisors, the alveolar sockets were implanted with TDM segments measuring 8 mm in length, either alone (TDM group) or seeded with dental pulp stem cells (TDM/DPSCs group). Animals were euthanized at two and six weeks post-implantation, specimens were processed for histological, histochemical and immunohistochemical analyses.
Results: TDM group at …
Feasibility Of Brief Behavioral Telehealth Interventions For Sleep And Pain In Adults With Chronic Musculoskeletal Orofacial Pain: An Idiographic Clinical Trial, Linda Sangalli, Gabriel Gilmore, Isabel Moreno-Hay, Lauren N. Whitehurst, Anna Alessandri-Bonetti, Ian Andres Boggero
Feasibility Of Brief Behavioral Telehealth Interventions For Sleep And Pain In Adults With Chronic Musculoskeletal Orofacial Pain: An Idiographic Clinical Trial, Linda Sangalli, Gabriel Gilmore, Isabel Moreno-Hay, Lauren N. Whitehurst, Anna Alessandri-Bonetti, Ian Andres Boggero
Oral Health Science Faculty Publications
Objective: Brief behavioral interventions targeting pain (Physical Self-Regulation, PSR) or sleep (Brief Behavioral Intervention for Insomnia, BBTI) show promise for interdisciplinary management of chronic pain, though their feasibility with chronic musculoskeletal orofacial pain (MSK-OFP) populations remains underexplored. This pilot study assessed the feasibility of a randomized clinical trial (RCT) of these interventions in a university-affiliated tertiary OFP clinic and explored clinical changes in pain, sleep, and other secondary outcomes.
Methods: Treatment-seeking participants (N=19) with chronic MSK-OFP and insomnia were randomized to three-session PSR or BBTI. At pre- and post-intervention, participants completed questionnaires (assessing pain intensity, insomnia symptomatology, sleep …
Molar–Incisor And Generalized Grade C Periodontitis: Distinct Microbiome–Immune Interactions Suggest Divergent Pathogenesis, Camila S. Stolf, Hélvis E. S. Paz, Murilo C. Paraluppi, Manuela Maria Viana Miguel, Mauro P. Santamaria, Mabelle F. Monteiro, Deyvid E. Amgarten, Raquel R. A. Franco, Luciana S. Branco-De-Almeida, Luciana M. Shaddox, Renato C. V. Casarin
Molar–Incisor And Generalized Grade C Periodontitis: Distinct Microbiome–Immune Interactions Suggest Divergent Pathogenesis, Camila S. Stolf, Hélvis E. S. Paz, Murilo C. Paraluppi, Manuela Maria Viana Miguel, Mauro P. Santamaria, Mabelle F. Monteiro, Deyvid E. Amgarten, Raquel R. A. Franco, Luciana S. Branco-De-Almeida, Luciana M. Shaddox, Renato C. V. Casarin
Oral Health Practice Faculty Publications
Aim:
Molar-Incisor (PerioC-MIP) and Generalized (PerioC-G) Grade C Periodontitis could have distinctive etiopathogenesis behind their unique clinical patterns. Thus, this study aimed to distinguish these two phenotypes by analyzing the subgingival metagenomic profile and the inflammatory markers levels.
Methods:
In this cross-sectional comparative study, Gingival Crevicular Fluid (GCF) and Subgingival Biofilm (SB) were collected from 18 PerioC-MIP North Americans and 14 periodontally healthy controls (HC) from the same location (HC-MIP) and 20 PerioC-G Brazilians and 20 controls (HC-G). From GCF, immunoenzymatic analysis was performed. SB functional and taxonomic bacterial content was determined using shotgun metagenomics sequencing.
Results:
Taxonomic results showed …
Laser Versus Fluoride Varnish In Management Of Dentine Hypersensitivity In Non-Carious Lesions: A Randomized Clinical Trial, Hend Sayed Ahmed, Mohamed Fouad Haridy, Hoda Fouda, Amr Adham Shalaby, Youssef M. Haridy
Laser Versus Fluoride Varnish In Management Of Dentine Hypersensitivity In Non-Carious Lesions: A Randomized Clinical Trial, Hend Sayed Ahmed, Mohamed Fouad Haridy, Hoda Fouda, Amr Adham Shalaby, Youssef M. Haridy
Dentistry
No abstract provided.
Effect Of Different Finishing And Polishing Systems On Surface Roughness And Color Stability Of Composite Cad/Cam Blocks (In-Vitro Study), Hend Sayed Ahmed, Mohamed Fouad Haridy, Noura Alzayyat, Reem Mohamed Gadao
Effect Of Different Finishing And Polishing Systems On Surface Roughness And Color Stability Of Composite Cad/Cam Blocks (In-Vitro Study), Hend Sayed Ahmed, Mohamed Fouad Haridy, Noura Alzayyat, Reem Mohamed Gadao
Dentistry
No abstract provided.
From State House To World Health Assembly: The Distinct Missions Of National And International Leadership In Oral Healthcare, Robert A. Faiella Dmd, Mmsc., Mba, William R. Calnon Dds, Facd
From State House To World Health Assembly: The Distinct Missions Of National And International Leadership In Oral Healthcare, Robert A. Faiella Dmd, Mmsc., Mba, William R. Calnon Dds, Facd
Journal of the American College of Dentists
Leadership in oral healthcare has long been treated as a skill assimilated over time through experience rather than intentionally defined, taught, and assessed. Unlike clinical practice, education, research, and infection control, leadership in oral healthcare lacks consistent standards despite its critical role in integrating these systems and guiding the profession’s future. As healthcare becomes increasingly collaborative and global, dentistry must deliberately develop leadership capabilities at multiple levels. This article examines leadership at the national and international levels, using the American Dental Association’s role within U.S. and global health and dental organizations as examples. It explores why these leadership contexts require …
Long-Lasting Impact Of Public Health Dentistry, Michal Herman Dds, Facd
Long-Lasting Impact Of Public Health Dentistry, Michal Herman Dds, Facd
Journal of the American College of Dentists
This article reflects on the enduring impact of public health dentistry through the author’s 25-year professional journey serving underserved and marginalized communities. It examines persistent barriers to equitable oral health care, including workforce shortages, inadequate insurance coverage, racial disparities, and limited access in rural and low-income populations. Drawing on experiences in nonprofit and community dentistry, the article highlights the importance of mentorship, advocacy, leadership, and community engagement in addressing oral health inequities. It argues that public health dentistry represents a form of professional altruism grounded in service and social responsibility, and calls on dental professionals to expand access, support workforce …
J Am Coll Dent 2026 92 3
Journal of the American College of Dentists
The Journal of the American College of Dentists (JACD) publishes scholarly articles, editorials, essays, and reflections addressing the ethical, professional, and social dimensions of dentistry. The Journal serves as a forum for advancing ethics, professionalism, leadership, and excellence within the dental community. By fostering dialogue across disciplines and perspectives, JACD provides readers with insights that encourage ethical, professional practice, lifelong learning, and service to the public, reinforcing dentistry’s commitment to advancing oral healthcare and the common good.
Dental Implantology: A Literature Review, Kylie Taylor
Dental Implantology: A Literature Review, Kylie Taylor
Undergraduate Honors Theses
Dental implant therapy is the process of replacing teeth as a form of treatment to improve quality of life, function, and esthetics. The success of the implant depends on the surgical technique, type of prosthetic, and factors related to individual patients. The most important factor for implant success is osseointegration. Osseointegration is a process in which success is determined by the bone quality, the surface characteristics of the implant and the surgical approach. Some approaches to improve the osseointegration of the implant are low-speed drilling, guided surgery, and reduced surgical trauma at the site. The impact of systemic conditions, health …
What Is A Standard? Perspectives On Acd’S Standards For Leadership In Oral Healthcare, N. Karl Haden Phd, Facd
What Is A Standard? Perspectives On Acd’S Standards For Leadership In Oral Healthcare, N. Karl Haden Phd, Facd
Journal of the American College of Dentists
The American College of Dentists’ new Standards for Leadership in Oral Healthcare mark the first attempt in dentistry to articulate standards specifically for leadership, complementing the broader orientation offered by the Ethics Handbook for Dentistry. This article explores the basic question, “What is a standard?” Close reading of the document indicates at least three distinct but related senses of the term: an expectation, a norm, and a guiding principle. The article offers an interpretation of how these three senses fit together coherently. Referencing the Handbook, the article argues that the leadership standards are most impactful only when habituated, when practiced …
American College Of Dentists Team Leadership Standards, Julie Connolly Dds Mph Ms Facd, Sreenivas Koka Dds Ms Phd Mba Mas Facd Fcgdent
American College Of Dentists Team Leadership Standards, Julie Connolly Dds Mph Ms Facd, Sreenivas Koka Dds Ms Phd Mba Mas Facd Fcgdent
Journal of the American College of Dentists
Leadership standards elevate the profession of dentistry by establishing how leaders can be effective and impactful. The American College of Dentists presents 10 carefully curated team leadership standards that serve as guiding principles for team leaders in dental settings. When these standards are followed, leaders increase the likelihood of creating and sustaining a high-performing, mission-focused team. The importance of each standard is described and, where appropriate, examples are provided.
Standards For Leadership In Oral Healthcare: Applying Local And Regional Standards In Practice, Teresa A. Dolan Dds, Mph, Facd, Robin Henderson Dds, Facd
Standards For Leadership In Oral Healthcare: Applying Local And Regional Standards In Practice, Teresa A. Dolan Dds, Mph, Facd, Robin Henderson Dds, Facd
Journal of the American College of Dentists
The dental profession has well-established standards governing clinical practice, education, and research, yet has lacked comparable standards to guide leadership behavior. The American College of Dentists (ACD) Standards for Leadership in Oral Healthcare Task Force developed a comprehensive framework spanning four levels: 1) core; 2) team; 3) local and regional; and 4) national and international. This article describes the local and regional standards, which include 10 standards organized into three themes—Building Foundation, Leading with Purpose, and Developing Others—and their application through interactive workshops at the ACD Spring Leadership Conference 2026. Using structured role-playing exercises, Section leaders and Regents stress-tested the …
J Am Coll Dent 2026 92 2b
Journal of the American College of Dentists
This special issue of the Journal of the American College of Dentists responds to a growing but long-underrecognized demand for humanities and ethics in dental education, practice, and scholarship. It emerges from the work of the National Collaborative on Humanities and Ethics in Dentistry (NCHED), a multi-institutional partnership formed in 2023 to address the persistent exclusion of dentistry from broader health humanities conversations. Through national webinars and scholarly collaboration, NCHED has demonstrated substantial interest in ethical reflection, historical analysis, narrative inquiry, and the arts as essential to understanding oral health and dental care.
The unexpectedly strong response to the call …
Letters To The Editor
Journal of the American College of Dentists
This section of the Journal of the American College of Dentists presents professional correspondence regarding previously published articles and issues relevant to dentistry, ethics, education, policy, and patient care. Letters to the editor provide readers an opportunity to offer scholarly critique, clarification, perspective, or additional insight, while author responses promote constructive academic dialogue and continued professional reflection.
The letters in this issue respond to the JACD article “The Racial Politics of Early Dentistry and the Terrible ‘Toothache Blues’” (Vol. 92, No. 2).
Does Dexmedetomidine Affect Opioid Consumption Following Orthognathic Surgery?, Allen F. Champion, Jung-Me Lee, Daniel Taub, Yu Jin Lee
Does Dexmedetomidine Affect Opioid Consumption Following Orthognathic Surgery?, Allen F. Champion, Jung-Me Lee, Daniel Taub, Yu Jin Lee
Department of Oral and Maxillofacial Surgery Faculty Papers
BACKGROUND: Despite its purported analgesic effects, controversy exists regarding dexmedetomidine's efficacy as an opioid-sparing anesthetic adjunct.
PURPOSE: The purpose of this study is to measure the association between dexmedetomidine (DEX) administration during orthognathic surgery and postoperative opioid consumption.
STUDY DESIGN, SAMPLE, AND SETTING: A single-blind randomized prospective cohort study was implemented. Patients consenting for orthognathic surgery at Thomas Jefferson University from January 2022 to October 2024 were screened. American Society of Anesthesiologists physical status I to III individuals aged ≥14 years were included. Exclusion criteria were revision surgery, recreational drug use, opioid prescription within 2 years, and chronic pain.
PREDICTOR …
Government And Insurance And Access (Cgia) Update, James Budd Mpp
Government And Insurance And Access (Cgia) Update, James Budd Mpp
The Journal of the Michigan Dental Association
The MDA Board of Trustees has established its 2026 legislative priorities, focusing on dental loss ratios, assignment of benefits, and virtual credit card payment reform. Key issues include addressing the workforce shortage, student debt, and improving Medicaid anesthesia reimbursement. The update also tracks several House Bills, such as those regarding voluntary license surrender (HB 4929) and foreign-trained dentist licensure (HB 4921), which the MDA opposes to maintain standards. Despite a slow legislative session in 2025, the MDA continues to advocate for transparency in third-party litigation and updates to specialty advertising rules. These efforts aim to protect the profession and ensure …
Is Being A Shareholder ‘In Name Only’ A Problem?, Daniel Schulte Jd
Is Being A Shareholder ‘In Name Only’ A Problem?, Daniel Schulte Jd
The Journal of the Michigan Dental Association
This column addresses the legal risks for Michigan dentists entering "shareholder in name only" agreements with non-dentist entities. Michigan law requires dental practices to be owned by licensed dentists to ensure professional control over public healthcare. Arrangements where management companies or manufacturers can reclaim stock for a nominal fee effectively strip the dentist of true ownership. This lack of control can lead to ethical conflicts, such as pressure to sell specific appliances regardless of professional judgment. Engaging in such schemes may endanger a dentist's license by thwarting state laws intended to protect clinical independence.
Does The Mda Still Offer A Staff Compensation Study?, Rachel Sinacola Dds, Ms, Angie Kanazeh
Does The Mda Still Offer A Staff Compensation Study?, Rachel Sinacola Dds, Ms, Angie Kanazeh
The Journal of the Michigan Dental Association
This Q&A section clarifies that the MDA no longer distributes staff compensation surveys due to 2023 FTC enforcement policy changes. However, members can access UCR fee analysis reports at a discount through PPO Negotiation Solutions. For dentists nearing retirement, the MDA offers retired and retired life membership categories, which provide dues discounts or exemptions for those no longer earning income from dentistry. The source also recommends Best Card for credit card processing to save an average of $6,496 annually. Finally, practitioners are reminded that licensure renewal must be completed through the MiPLUS platform, which primarily issues electronic licenses.
The Evolving Jacd, Toni Roucka
The Evolving Jacd, Toni Roucka
Journal of the American College of Dentists
The Editorial is a feature in each issue of the JACD. It includes commentary on issue content, relevant topics, or other items of interest to the fellowship.
Unpacking And Re-Imagining Dental Ethics With Anti-Oppressive And Interdisciplinary Praxis, Eleanor Fleming Phd, Dds, Mph, Ficd, Facd
Unpacking And Re-Imagining Dental Ethics With Anti-Oppressive And Interdisciplinary Praxis, Eleanor Fleming Phd, Dds, Mph, Ficd, Facd
Journal of the American College of Dentists
Dental ethics are assumed to be neutral in their intent. With a social contract grounded in liberalism and assumptions centering whiteness, the resulting ethics reinforce hierarchy, otherness, and inequities. Because concepts and practices are rarely neutral, dental ethics is embedded with biases, which results in it falling short of the demands of the 21st century and exacerbating health inequities rather than supporting efforts for health justice. This manuscript invites the reader to reflect critically and re-imagine dental ethics using anti-oppressive and interdisciplinary praxis. For the improvement of public health, the narrowing of inequities, and a functioning oral health system, the …
Dr. William Thomas Green Morton, Civil War Hero, Peter H. Jacobsohn Dds, Facd
Dr. William Thomas Green Morton, Civil War Hero, Peter H. Jacobsohn Dds, Facd
Journal of the American College of Dentists
This article examines the overlooked Civil War contributions of Dr. William Thomas Green Morton, the dentist best known as the Father of Surgical Anesthesia. While Morton’s role in introducing ether anesthesia to surgery is well documented, his service as a volunteer anesthetist for the Union Army remains largely unrecognized in Civil War literature. Drawing on contemporary accounts, letters, and eyewitness descriptions, the article highlights Morton’s presence on major battlefields, where he administered ether to thousands of wounded soldiers. His work dramatically reduced surgical pain, improved morale, and marked the earliest example of organized military anesthesia in American history.
Moving Forward With Purpose: Aadej Adopts New Mission, Vision, And Values Statements, Jasmine Shafagh Dmd
Moving Forward With Purpose: Aadej Adopts New Mission, Vision, And Values Statements, Jasmine Shafagh Dmd
AADEJ - The Communicator
Regular feature from the president of AADEJ, Dr. Jasmine R. Shafagh with the latest updates from AADEJ.
Dual Regulation Of Osteogenesis And Inflammation By Pomegranate (Punica Granatum L.) Extract In Periodontal Ligament-Derived Stem Cells: Implications For Regenerative Medicine, Mai Hafez
Dentistry
Objectives
This article aims to compare the osteogenic along with anti-inflammatory activity of different concentrations of pomegranate extract (PG) on human periodontal ligament-derived stem cells (hPDLSCs) in vitro.
Materials and Methods
hPDLSCs were acquired from the root surface of removed molars, cultured, and then distinguished by flow cytometry analysis and several lineage differentiation potentials. To identify the effect of PG on hPDLSCs' viability, an MTT assay was performed. hPDLSCs were maintained in osteogenic induction medium with varying concentrations of PG. At the end of the induction period, osteogenesis was assessed using Alizarin Red staining, ALP assay, and qRT-PCR to determine …
Cytocompatibility, Osteogenic Potential, Antibacterial And Antibiofilm Efficacy Of A Calcium-Silicate-Based Intracanal Medication, Shehabeldin Mohamed Saber Prof.Dr.
Cytocompatibility, Osteogenic Potential, Antibacterial And Antibiofilm Efficacy Of A Calcium-Silicate-Based Intracanal Medication, Shehabeldin Mohamed Saber Prof.Dr.
Dentistry
This study assessed the biological and antimicrobial properties of a calcium silicate-based material and calcium hydroxide (CH) when used as intracanal medications. MTT assay, osteogenic differentiation of human periodontal stem cells (hPDLSCs), cell mineralization-assay, and determination of ALP activity were assessed to investigate the biological properties. While the agar well diffusion, crystal violet (CRV) assay and LIVE/DEAD staining of dentin slices infected with a mature E. faecalis biofilm were used to assess the antimicrobial properties. Normally distributed data were analyzed using one and two-way ANOVA and post hoc Tukey test, while for non-normally distributed data Kruskal Wallis and Dunn's tests …