Dentistry Commons^™

Adult Permanent (Extracted) Teeth As A Source Of Dental Pulp-Derived Mesenchymal Stem Cells (Dpsc): A Pilot Study, Charles Hill

UNLV Theses, Dissertations, Professional Papers, and Capstones

Mesenchymal stem cells (MSC) may be derived from a variety of adult human tissues, including dental pulp from extracted or exfoliated teeth. Evidence suggests differences in both the quality and quantity of the dental pulp-derived mesenchymal stem cells (DPSC) obtained from different sources, such as primary or "baby teeth" and adult, permanent teeth. This study aimed to evaluate the potential to obtain DPSC from intact, vital permanent teeth, and characterize the potential isolates using a randomized selection of active dental patients from the University of Nevada, Las Vegas School of Dental Medicine (UNLVSODM). DPSC were extracted, isolated, cultured and characterized …

Ccl3 And Cxcl12 Production In Vitro By Dental Pulp Fibroblasts From Permanent And Deciduous Teeth Stimulated By Porphyromonas Gingivalis Lps, Carla Renata Sipert, Ana Carolina Morandini, Karin Cristina Da Silva Modena, Thiago José Dionísio, Maria Aparecida Andrade Moreira Machado, Sandra Helena Penha Oliveira, Ana Paula Campanelli, Carlos Ferreira Santos

All Dugoni School of Dentistry Faculty Articles

OBJECTIVE: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS).

MATERIAL AND METHODS: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0-10 µg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples …