Dentistry Commons^™

Antimicrobial Peptide Coating Of Dental Implants: Biocompatibility Assessment Of Recombinant Human Beta Defensin-2 For Human Cells, Patrick Warnke, Eske Voss, Paul Russo, Sebastien Stephens, Michael Kleine, Hendrik Terheyden, Qin Liu

Qin Liu

Purpose: Artificial materials such as dental implants are at risk of bacterial contamination in the oral cavity. Human beta defensins (HBDs), small cationic antimicrobial peptides that exert a broad-spectrum antibacterial function at epithelial surfaces and within some mesenchymal tissues, could probably help to reduce such contamination. HBDs also have protective immunomodulatory effects and have been reported to promote bone remodeling. The aim of this study, therefore, was to investigate the influence of recombinant HBD-2 on the proliferation and survival of cells in culture.

Materials and Methods: Human mesenchymal stem cells (hMSCs), human osteoblasts, human keratinocytes (control), and the HeLa cancer …

Comparison Of In Vitro Biocompatibility Of Nanobone(®) And Biooss(®) For Human Osteoblasts, Qin Liu, Timothy Douglas, Christiane Zamponi, Stephan Becker, Eugene Sherry, Sureshan Sivananthan, Frauke Warnke, Jörg Wiltfang, Patrick Warnke

Qin Liu

Introduction:Scaffolds for bone tissue engineering seeded with the patient's own cells might be used as a preferable method to repair bone defects in the future. With the emerging new technologies of nanostructure design, new synthetic biomaterials are appearing on the market. Such scaffolds must be tested in vitro for their biocompatibility before clinical application. However, the choice between a natural or a synthetic biomaterial might be challenging for the doctor and the patient. In this study, we compared the biocompatibility of a synthetic bone substitute, NanoBone®, to the widely used natural bovine bone replacement material BioOss®.Material and methods: The in …

Proliferation Assessment Of Primary Human Mesenchymal Stem Cells On Collagen Membranes For Guided Bone Regeneration, Qin Liu, A. Humpe, D. Kletsas, F. Warnke, S. Becker, T. Douglas, S. Sivananthan, P. Warnke

Qin Liu

Purpose: Human mesenchymal stem cells (hMSCs) hold the potential for bone regeneration because of their self-renewing and multipotent character. The goal of this study was to evaluate the influence of collagen membranes on the proliferation of hMSCs derived from bone marrow. A special focus was set on short-term eluates derived from collagen membranes, as volatile toxic materials washed out from these membranes may influence cell behavior during the short time course of oral surgery.

Materials and Methods: The proliferation of hMSCs seeded directly on a collagen membrane (BioGide) was evaluated quantitatively using the cell proliferation reagent WST-1 (4-3-[4-iodophenyl]-2-[4-nitrophenyl]-2H-[5-tetrazolio]-1, …