Involvement Of Signal Peptidase I In Streptococcus Sanguinis Biofilm Formation, Jessica Aynapudi

Theses and Dissertations

Biofilm accounts for 65%-80% of microbial infections in humans. Considerable evidence links biofilm formation to oral disease and consequently systemic infections. Eradication of biofilm-associated infections is important. Streptococcus sanguinis, a Gram-positive bacterium, is one of the most abundant species in oral biofilm. It contributes to biofilm development in oral cavities and is one of the recognized causes of infective endocarditis. To study and identify biofilm genes in S. sanguinis, biofilm formation of 51 mutants was compared with the wild type SK36 strain using crystal violet (CV) staining in a microtiter plate. Confocal laser scanning microscopy (CLSM) and image analysis was …

Vital Pulp Therapy Survivability Based On Radiographic Depth Of Caries, Aaron T. Schmick

Theses and Dissertations

Purpose: The purpose of this retrospective chart review was to determine if the survival of two methods of vital pulp therapy (VPT) were influenced by the pre-operative radiographic depth and location of caries. Methods: Electronic patient records (axiUm®) that contained the procedure codes D3120, Indirect Pulp Therapy (IPT), or D3220, Therapeutic Pulpotomy (TP), were queried. Qualifying charts’ pre-operative and post-operative radiographs were viewed in MiPACS® by two raters. Visit records were queried again to identify any other treatment failures. Results: A total of 568 primary molars met the eligibility criteria. There was a difference in survival depending upon the treatment …

The Effectiveness Of A Preventive Recall Strategy In Children Following Dental Rehabilitation Under General Anesthesia, Amanda Kerns Dr.

Theses and Dissertations

Abstract

THE EFFECTIVENESS OF A PREVENTIVE RECALL STRATEGY IN CHILDREN FOLLOWING DENTAL REHABILITATION UNDER GENERAL ANESTHESIA

By Amanda Kerns, DDS

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Dentistry at Virginia Commonwealth University.

Virginia Commonwealth University, 2016

Thesis Advisor: Elizabeth Berry, DDS, MPH, MSD

Vice Chair, Assistant Professor, Department of Pediatric Dentistry

Purpose: This was a prospective randomized controlled trial assessing the impact of a preventive strategy following full-mouth dental rehabilitation (FMDR) in children with early childhood dental caries.

Methods: 130 patients completed FMDR and were included in the analysis. …

Serum Vitamin D, Pth, And Calcium Levels In Patients With And Without Early Childhood Caries, Susan A. Meinerz, Harmeet Chiang, Peter C. Moon, Lorin M. Bachmann, Tegwyn Brickhouse, Al M. Best, Tiffany Williams

Theses and Dissertations

Purpose: The purpose was to determine differences in serum vitamin D, parathyroid hormone (PTH), and calcium levels between patients with early childhood caries (ECC) and patients without dental decay.

Materials and Methods: Serum vitamin D, PTH, and calcium levels were obtained from 30 children without dental decay who acted as controls and 60 children with ECC. A questionnaire was filled out by the parent/guardian of each participant consisting of questions regarding medical and dental history, exposure to sources of vitamin D and demographic information.

Results: The difference in the vitamin D levels of the participants was most …

Social Determinants Of Participation In A Home Visitation Fluoride Varnish Program, James E. Puryear, Tegwyn Brickhouse, Caroline Carrico

Theses and Dissertations

Purpose: The purpose of this study is to examine the social determinants of CHIP (Child Health Investment Partnership) of Roanoke Valley children who participated in the preventive oral health program compared to those who did not.

Methods: This is a retrospective cohort study of children (n=2,425) enrolled in CHIP of Roanoke Valley from September 2008-September 2014. Bivariate analysis and multivariable logistic regression models were used to compare age, gender, race, locality, parents’ education level, age at enrollment, and length of enrollment for oral health program participants versus those who did not participate.

Results: Children who were Hispanic …

Parental Perceptions Of Oral Health Related Quality Of Life For Children That Receive Care On Give Kids A Smile Day, Andrew Gibson

Theses and Dissertations

Purpose: The purpose of this study is to evaluate the oral health-related quality of life for patients treated at Give Kids a Smile. Methods: Participants were asked to complete a 25-question survey regarding their child’s oral health-related quality of life (OHRQoL), with answers ranked on a 0 to 4 point scale. Results: A total of 78 questionnaires were completed, with the mean score of 5.19. Conclusions: Give Kids a Smile was created to treat children with unmet oral healthcare needs, therefore it was hypothesized that the OHRQoL for the children treated would be negatively impacted and thus this score high. …

Assessment Of Changes In The Size Of Periapical Radiolucencies 3-12 Months Post Non-Surgical Root Canal Treatment Using Cbct Imaging: A Pilot Study, Jeremy W. Fike Dds

Theses and Dissertations

The purpose of this study was to assess the changes in size of periapical lesions 3-12 months following root canal treatment using CBCT. Patients who had non-surgical root canal therapy (NSRCT) or non-surgical retreatment (ReTx) from July 30,2014 to August 19, 2015 with a periapical lesion of endodontic origin and received NSRCT or ReTx and had a pre-treatment or intra-treatment CBCT were invited to participate. Volumetric and linear measurements of periapical lesions on initial and post- treatment CBCT images were performed. A total of 20 patients with 23 treated teeth with 30 separate periapical radiolucent lesions returned for follow up …

Analysis Of Radiographic Changes Associated With The Periradicular Diagnosis Of Symptomatic Apical Periodontitis, Preeti Batra

Theses and Dissertations

Symptomatic apical periodontitis (SAP) is indicative of inflammation of the periodontal ligament. It may or may not be accompanied by radiographic changes and may occur with vital or necrotic pulpal diagnosis. Purpose of this study was to analyze clinical and radiographic presentations of SAP in a retrospective electronic dental chart and digital radiograph review utilizing the endodontic diagnostic template note of predoctoral dental school patients presenting with SAP. The aim was to determine prevalence of periapical radiolucencies (PARLs) in SAP, association of SAP to pulpal diagnosis, and define associated radiographic changes. Most prevalent pulpal diagnosis with SAP was symptomatic irreversible …

Perception Of Differences In Lip Profile Between 2-D And 3-D, Andrew Hansen

Theses and Dissertations

Past studies evaluating the esthetics of orthodontic treatment have been done using 2-D images. New 3-D imaging offers an improved, real-life representation of a subject. The purpose of this study was to determine how laypeople perceived differences in lip position (flat versus ideal lip fullness) in 2-D compared to 3-D.

3dMD images of 8 Caucasian subjects were adjusted to an ideal and flat lip position in 3-D and then in 2-D from the profile view. 2 surveys were created with paired ideal and flat images on the screen, either in 2-D or 3-D, and evaluators were asked to choose which …

The Study Of The Regulon Of Oxyr In Escherichia Coli And Porphyromonas Gingivalis, Christopher K. Pham

Theses and Dissertations

The facultative anaerobe, Escherichia coli and the obligate anaerobe, Porphyromonas gingivalis are two bacteria that reside in our body. Although they reside in separate environments, they are both subject to hydrogen peroxide stress and have mechanisms to regulate the stress. OxyR is the primary transcriptional regulator/sensor of oxidative stress response caused by hydrogen peroxide. OxyR in P. gingivalis is not well-characterized compared to OxyR in E. coli. We sought to characterize and compare the two forms of OxyR in order to gain a better understanding of the protein. We determined the oligomeric state of both proteins: primarily a tetramer for …

Twitter Analysis Of The Orthodontic Patient Experience With Braces Versus Invisalign, Daniel A. Noll

Theses and Dissertations

The purpose of this study was to examine the orthodontic patient experience with braces compared to Invisalign^® by means of a large-scale Twitter sentiment analysis. A custom data collection program was created to collect tweets containing the words “braces” or “Invisalign.” A hierarchal Naïve Bayes sentiment classifier was developed to sort the tweets into one of five categories: positive, negative, neutral, advertisement, or not applicable. Among the 419,363 tweets applicable to orthodontics collected, users posted significantly more positive tweets (61%) than negative tweets (39%) (p-value = ^® tweets (p-value=0.4189). In conclusion, Twitter users express more positive than negative sentiment …

Perception Of Profile Appearance As Judged By Peers Using 3d Video Imaging, Megan G. Schuler

Theses and Dissertations

The purpose of this study was to investigate the social perceptions of subjects with differing lip position and facial convexity in three dimensions. A 3dMD camera (3dMD, Atlanta, GA) was used to capture 3D images of 9 subjects’ faces. The images were altered to have ideal lip position and ideal convexity, ideal lip position and Class II convexity, Class II lip position and ideal convexity, and Class II lip position and Class II convexity. 400 laypersons rated their perceptions of the subjects’ athletic ability, popularity, leadership, and intelligence on a VAS scale. Subjects with ideal lip position relative to the …

Comparison Of Treatment Management Between Orthodontists And General Practitioners Performing Clear Aligner Therapy, Alexandra D. Best

Theses and Dissertations

The purpose of this study was to investigate differences in case confidence, treatment management, and Invisalign® expertise between orthodontists and general dentists. A survey was mailed to 1,000 randomly selected orthodontists and general dentists, respectively, who are Invisalign® providers, and results were analyzed. The results indicated that orthodontists treated significantly more Invisalign® cases and received more Invisalign® training than general dentists (P

Incorporation Of Interprofessional Education And Special Care Dentistry Into Standard Dental School Curriculum, Marshall D. Adzima

Undergraduate Research Posters

According to dentistry, special needs is defined as individuals whose medical, physical, psychological, or social situations may make it necessary to modify normal dental routines in order to provide dental treatment for that individual. The population of special needs patients is increasing. The lack of educated professionals in special care dentistry is leading to severe and untreated systemic and oral health problems in the special needs population. The implementation of interprofessional treatment for special needs individuals has proven to be the most effective method of treatment within the special needs population. This study examines the current application of special care …

Identification Of Small-Molecule Inhibitors Against Meso-2, 6-Diaminopimelate Dehydrogenase From Porphyromonas Gingivalis, Victoria N. Stone, Hardik I. Parikh, Fadi El-Rami, Xiuchun Ge, Weihau Chen, Yan Zhang, Glen E. Kellogg, Ping Xu

Philips Institute for Oral Health Research Publications

Species-specific antimicrobial therapy has the potential to combat the increasing threat of antibiotic resistance and alteration of the human microbiome. We therefore set out to demonstrate the beginning of a pathogen-selective drug discovery method using the periodontal pathogen Porphyromonas gingivalis as a model. Through our knowledge of metabolic networks and essential genes we identified a “druggable” essential target, meso-diaminopimelate dehydrogenase, which is found in a limited number of species. We adopted a high-throughput virtual screen method on the ZINC chemical library to select a group of potential small-molecule inhibitors. Meso-diaminopimelate dehydrogenase from P. gingivaliswas first expressed …

Chemokine Function In Periodontal Disease And Oral Cavity Cancer, Sinem Esra Sahingur, W. Andrew Yeudall

Periodontics Publications

The chemotactic cytokines, or chemokines, comprise a superfamily of polypeptides with a wide range of activities that include recruitment of immune cells to sites of infection and inflammation, as well as stimulation of cell proliferation. As such, they function as antimicrobial molecules and play a central role in host defenses against pathogen challenge. However, their ability to recruit leukocytes and potentiate or prolong the inflammatory response may have profound implications for the progression of oral diseases such as chronic periodontitis, where tissue destruction may be widespread. Moreover, it is increasingly recognized that chronic inflammation is a key component of tumor …

Virginia Dental Journal (Vol. 92, No. 1, 2015)

Virginia Dental Journal

No abstract provided.

Virginia Dental Journal (Vol. 92, No. 4, 2015)

Virginia Dental Journal

No abstract provided.

Virginia Dental Journal (Vol. 92, No. 3, 2015)

Virginia Dental Journal

No abstract provided.

Osseointegration Of Wrapped Dental Implants In Rabbits, Alex Whitehead

AUCTUS: The Journal of Undergraduate Research and Creative Scholarship

Edentulous patients (those lacking teeth) require one of two approaches to augmentation: inserting a vertical bone graft, or subperiosteally anchoring a device on which an implant can be attached. Bone grafts have had unpredictable results and can undergo resorption over time, compromising implant stability and success. As of the subperiosteal approach, current materials have not proven to directly integrate with the bone, in a process termed osseointegration. Therefore, we used additive manufacturing to create a Ti6Al4V alloy surface with a specific roughness to determine if it would be osseoinductive in a challenging rabbit model over six weeks.

Computer Simulation Of Tooth Mobility Using Varying Material Properties, Allison R. Beckmann, Lena Unterberg, Stefan Raith, Horst Fischer

AUCTUS: The Journal of Undergraduate Research and Creative Scholarship

Tooth mobility is the major cause of stress on a tooth implant or partial denture and often results in the damage of the device. For many prosthetic devices like dental bridges, a partial denture used for a person who is missing a tooth to give the appearance and function of a tooth, mobility can cause up to double the amount of stress in comparison to a fixed model. Creating a computer simulation of tooth mobility using Finite Element Analysis allows one to understand and predict this movement in order to improve future dental prosthetic devices. The main cause of tooth …

A Justification For The Trend Towards Indirect Pulp Therapy, Marissa Kuhnen

Theses and Dissertations

Purpose: The purpose of this retrospective chart review was to determine how primary molars needing vital pulp therapy have been treated in the past four years at Virginia Commonwealth University (VCU) and to determine which treatments: indirect pulp therapy (IPT), formocresol pulpotomy, and ferric sulfate pulpotomy have been successful. Methods: AxiUm records that contained the procedure codes D3120 (Pulp Cap – Indirect) or D3220 (Therapeutic Pulpotomy) were totaled by year. Visit records were queried again to identify treatment failures i.e. extractions or pulpectomy. Results: In 2010, 52% of vital pulp therapies were ferric sulfate pulpotomies and in …

Crown Size Comparisons In Patients With Unilateral Palatally Displaced Canines, Joseph Eliason, Steven J. Lindauer

Theses and Dissertations

Abstract

CROWN SIZE COMPARISONS IN PATIENTS WITH PALATALLY DISPLACED CANINES

By Joseph L. Eliason, D.D.S.

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Dentistry at Virginia Commonwealth University.

Virginia Commonwealth University, 2015

Thesis Director: Steven J. Lindauer, D.M.D., M.Dent.Sc.

Professor and Chair, Department of Orthodontics

There has been significant debate over the past decades regarding the etiology of palatally displaced canines. Theorized risk factors include agenesis or malformation of the lateral incisors, incisor retroclination, transverse deficiency, or genetic predisposition. The purpose of this study is to compare the linear and volumetric …

A Comparison Of Vitamin D Levels In Children With Early Childhood Caries, Vanessa O. Hofilena

Theses and Dissertations

Purpose: To determine if there is an association between early childhood caries (ECC) and vitamin D deficiency, as measured via a serum sample. An exploratory goal was to investigate the possibility of measuring vitamin D with a salivary assay. Methods: Serum samples of patients who were scheduled for a dental or otolaryngology procedure under general anesthesia were assessed for 25-hydroxyvitamin D (25(OH)D), parathyroid hormone and calcium. Results: Our analysis indicates that the vitamin D levels of the controls and ECC group were significantly different. There were significant inverse correlations between: 1) PTH levels and vitamin D and 2) decayed, missing, …

Motivational Factors For Treating Patients With Special Health Care Needs, Arpi Patel

Theses and Dissertations

Purpose: The purpose of this study is to assess what training and motivational factors dental providers report in providing dental care to PSHCN (patients with special healthcare needs. Materials and Methods: An electronic questionnaire was sent to n=104 fourth year dental students, n=147 general dentists with a specific continuing education course pertaining to PSCHN, and n=140 pediatric dentists in Virginia. The questionnaire consisted of four sections including Demographics, Professional Attitudes, Special Needs Patients and Motivational Factors, and PSCHN Cases. Results: The overall response rate for our study was 21%. The response rates of dental students, general dentists, …

Virginia Dental Journal (Vol. 92, No. 3 [I.E. 2], 2015)

Virginia Dental Journal

No abstract provided.

The Prevalence, Predictive Factors, And Classification Of Intrapulpal Cracks In Maxillary Premolars Requiring Endodontic Treatment, Sarah Krygowski

Theses and Dissertations

Cracked teeth may be difficult to diagnose. Craze lines rarely become symptomatic or require treatment. Cracks in the enamel and dentin alone may or may not become symptomatic and require restorative treatment. However, cracks extending into the enamel, dentin, and pulp chamber provide an avenue for bacteria to establish infection and this commonly results in symptoms and the need for endodontic and restorative treatment. The published endodontic literature has limited information regarding the prevalence or predictive factors for cracks extending into the pulp chamber of teeth. The purpose of this study was to determine the prevalence and classification of intrapulpal …

Lowering The Number Of False Positive Responses To Electric Pulp Tests By Qualifying Patient Response, Katherine Js Lee

Theses and Dissertations

The electric pulp test (EPT) has been shown to be a valuable tool in assessing pulp vitality. One of its drawbacks, however, is its high number of false positives. The purpose of this study was to determine if qualifying the type of sensation elicited by the test could lower the percentage of false positive responses. A retrospective chart review of an eleven-month period was conducted, examining all patients with necrotic teeth tested with the EPT. Of 189 teeth, when the patient responded to EPT at first sensation 23.3% of teeth were incorrectly identified as vital. When the patient was asked …

The Role Of Leukocyte-Platelet Rich Fibrin In Human Alveolar Ridge Preservation: A Randomized Clinical Trial, Thomas F. Glazier, Thomas C. Waldrop, John C. Gunsolley, Robert Sabatini

Theses and Dissertations

PURPOSE: The aim of this study is to examine the healing of intact extraction sockets grafted with leukocyte-platelet rich fibrin (L-PRF) as compared to sockets grafted with freeze-dried bone allograft (FDBA) and a resorbable collagen barrier membrane (RCM).

METHODS: This prospective randomized, examiner blinded pilot study included 17 subjects randomized to two treatment groups. Serum total cholesterol, low density lipoprotein (LDL), 25-hydroxyvitamin-D₃, and platelet counts were determined preoperatively in all subjects. The experimental arm consisted of 8 posterior tooth-bounded intact extraction sites receiving L-PRF plugs. The control group consisted of 9 posterior tooth-bounded intact extraction sites receiving FDBA …

Esthetic Preferences Of Maxillary Incisor Labiolingual Inclination Across Races, Elvi M. Barcoma

Theses and Dissertations

Abstract

ESTHETIC PREFERENCES FOR MAXILLARY INCISOR LABIOLINGUAL INCLINATION ACROSS RACES

by Elvi Marie Barcoma, D.D.S.

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Dentistry at Virginia Commonwealth University

Virginia Commonwealth University, 2015

Major Director: Bhavna Shroff, D.M.D., M.Dent.Sc, Program Director, Orthodontics

Objective: To determine if people of different racial backgrounds prefer different amounts of

maxillary incisor labiolingual inclination from a smiling profile view.

Materials and Methods: An electronic survey was created with smiling profile images of an African-American female and a White female with varying degrees of maxillary incisor labiolingual inclination. …