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Full-Text Articles in Dentistry
Differential Involvement Of The Canonical And Noncanonical Inflammasomes In The Immune Response Against Infection By The Periodontal Bacteria Porphyromonas Gingivalis And Fusobacterium Nucleatum, Kívia Queiroz De Andrade, Cassio Luiz Coutinho Almeida-Da-Silva, David M. Ojcius, Robson Coutinho-Silva
Differential Involvement Of The Canonical And Noncanonical Inflammasomes In The Immune Response Against Infection By The Periodontal Bacteria Porphyromonas Gingivalis And Fusobacterium Nucleatum, Kívia Queiroz De Andrade, Cassio Luiz Coutinho Almeida-Da-Silva, David M. Ojcius, Robson Coutinho-Silva
All Dugoni School of Dentistry Faculty Articles
We examined the involvement of the P2 × 7 receptor and the canonical and noncanonical inflammasomes in the control of single-species or dual-species infection by the periodontal bacteria Porphyromonas gingivalis and Fusobacterium nucleatum in cells and mice. Stimulation of the P2 × 7 receptor leads to activation of the canonical NLRP3 inflammasome and activation of caspase-1, which leads to cleavage of pro-IL-1β to IL-1β, a key cytokine in the host inflammatory response in periodontal disease. The non-canonical inflammasome pathway involves caspase-11. Thus, wildtype (WT), P2 × 7−/−, caspase-11−/− and caspase-1/11−/− mice were co-infected with both bacterial …
Structural And Functional Features Of The P2x4 Receptor: An Immunological Perspective, Jean M. Kanellopoulos, Cássio Luiz Coutinho Almeida-Da-Silva, Sirje Rüütel Boudinot, David M. Ojcius
Structural And Functional Features Of The P2x4 Receptor: An Immunological Perspective, Jean M. Kanellopoulos, Cássio Luiz Coutinho Almeida-Da-Silva, Sirje Rüütel Boudinot, David M. Ojcius
All Dugoni School of Dentistry Faculty Articles
Extracellular nucleotides are important mediators of activation, triggering various responses through plasma membrane P2 and P1 receptors. P2 receptors are further subdivided into ionotropic P2X receptors and G protein-coupled P2Y receptors. P2X4 is an ATP-gated cation channel broadly expressed in most tissues of the body. Within the P2X family, P2X4 has a unique subcellular distribution, being preferentially localized in lysosomes. In these organelles, high ATP concentrations do not trigger P2X4 because of the low pH. However, when the pH increases to 7.4, P2X4 can be stimulated by intra-lysosomal ATP, which is in its active, tetra-anionic form. Elucidation of P2X4, P2X3 …