Dentistry Commons^™

Activation Of Extracellular-Signal Regulated Kinase (Erk1/2) By Fluid Shear Is Ca2+- And Atp-Dependent In Mc3t3-E1 Osteoblasts, Dawei Liu, Damian C. Genetos, Ying Shao, Derik J. Geist, Jiliang Li, Hua Zhe Ke, Charles H. Turner, Randall L. Duncan

School of Dentistry Faculty Research and Publications

To determine the role of Ca²⁺ signaling in activation of the Mitogen-Activated Protein Kinase (MAPK) pathway, we subjected MC3T3-E1 pre-osteoblastic cells to inhibitors of Ca²⁺ signaling during application of fluid shear stress (FSS). FSS only activated ERK1/2, rapidly inducing phosphorylation within 5 min of the onset of shear. Phosphorylation of ERK1/2 (pERK1/2) was significantly reduced when Ca_i²⁺ was chelated with BAPTA or when Ca²⁺ was removed from the flow media. Inhibition of both the L-type voltage-sensitive Ca²⁺ channel and the mechanosensitive cation-selective channel blocked FSS-induced pERK1/2. Inhibition of phospholipase C with U73122 significantly reduced …

Socio-Demographics Of Adult Orthodontic Visits In The United States, Joseph Whitesides, Nicholas M. Pajewski, T. Gerard Bradley, Anthony Iacopino, Christopher Okunseri

School of Dentistry Faculty Research and Publications

Introduction: Population-based studies in orthodontics have focused on differences between normative and perceived needs. However, information from national data on the prevalence of orthodontic visits and their associated factors in adults in the United States is scarce. We examined the demographic profile of likely adult users of orthodontic services and whether there is racial and ethnic disparity in orthodontic visits. Methods: We analyzed data from the Medical Expenditure Panel Survey, 2000-2004. Results: Overall, about 1% of the population reported an orthodontic visit. Subjects who made a general dental visit during the current year were significantly more likely to also have …

Bond Strength Of An Amorphous Calcium Phosphate–Containing Orthodontic Adhesive, Jeff A. Foster, David W. Berzins, T. Gerard Bradley

School of Dentistry Faculty Research and Publications

Objective: To determine whether an amorphous calcium phosphate (ACP)-containing adhesive has an acceptable level of shear bond strength to be used as an orthodontic adhesive.

Materials and Methods: Sixty extracted premolars were randomly divided into three groups for orthodontic bonding. Group 1 used a composite resin adhesive (Transbond XT), group 2 was bonded with an ACP-containing adhesive (Aegis Ortho), and group 3 used a resin-modified glass ionomer (Fuji Ortho LC). All bonded teeth were stored in distilled water at 37°C for 40 ± 2 hours prior to debonding. Shear bond strength and adhesive remnant index (ARI) were recorded for each …

Hierarchical Cluster Analysis Of Myoepithelial/Basal Cell Markers In Adenoid Cystic Carcinoma And Polymorphous Low-Grade Adenocarcinoma, Manju L. Prasad, Catalin C. Barbacioru, Yeshwant B. Rawal, Omar Husein, Ping Wen

School of Dentistry Faculty Research and Publications

Distinguishing adenoid cystic carcinoma from polymorphous low-grade adenocarcinoma of the salivary glands is important for their management. We studied the expression of several myoepithelial and basal/stem cell markers (smooth muscle actin, calponin, smooth muscle myosin heavy chain, metallothionein, maspin, and p63) by immunohistochemistry in 23 adenoid cystic carcinoma and 24 polymorphous low-grade adenocarcinoma, to identify the most useful marker or combination of markers that may help their diagnoses. The results were analyzed using hierarchical cluster analysis and χ² test for trend. We noted diffuse expression of smooth muscle actin in 20 adenoid cystic carcinoma vs one polymorphous low-grade adenocarcinoma …

Hmong Adults Self-Rated Oral Health: A Pilot Study, Christopher Okunseri, Marcie Yang, Cesar D. Gonzalez, Warren Lemay, Anthony Iacopino

School of Dentistry Faculty Research and Publications

Since 1975, the Hmong refugee population in the U.S. has increased over 200%. However, little is known about their dental needs or self-rated oral health (SROH). The study aims were to: (1) describe the SROH, self-rated general health (SRGH), and use of dental/physician services; and (2) identify the factors associated with SROH among Hmong adults. A cross-sectional study design with locating sampling methodology was used. Oral health questionnaire was administered to assess SROH and SRGH, past dental and physician visits, and language preference. One hundred twenty adults aged 18–50+ were recruited and 118 had useable information. Of these, 49% rated …

Novel Molecules For Intra-Oral Delivery Of Antimicrobials To Prevent And Treat Oral Infectious Diseases, Periathamby A. Raj, Latha Rajkumar, Andrew R. Dentino

School of Dentistry Faculty Research and Publications

New molecules were designed for efficient intra-oral delivery of antimicrobials to prevent and treat oral infection. The salivary statherin fragment, which has high affinity for the tooth enamel, was used as a carrier peptide. This was linked through the side chain of the N-terminal residue to the C-terminus of a defensin-like 12-residue peptide to generate two bifunctional hybrid molecules, one with an ester linkage and the other with an anhydride bond between the carrier and the antimicrobial components. They were examined for their affinity to a HAP (hydroxyapatite) surface. The extent of the antimicrobial release in human whole saliva was …

Presenca De Dentes Permanentes Irrompidos Em Escolares Da Regiao Metropolitana De Porto Alegre, Karine Squeff, Guilherme Pessoa Cerveira, Luciane Quadrado Closs, Jose A. Bosio

School of Dentistry Faculty Research and Publications

Foi realizada uma pesquisa de campo em duas escolas da rede pública estadual de Porto Alegre, com a finalidade de verificar a presença de dentes permanentes irrompidos em crianças com faixa etária entre seis a dez anos. Foram examinadas 327 crianças, considerando as variáveis de idade, sexo e raça. Não foi observada diferença significativa para a época de erupção para as variáveis de raça e sexo. Foi observado que aos seis anos de idade uma porcentagem maior de incisivos inferiores (78,7%) irrompeu antes que os primeiros molares inferiores (75,9%), assim como a erupção na arcada inferior antecedeu a da arcada …

A Nodular Submental Mass, Yeshwant B. Rawal, Carl M. Allen, John R. Kalmar

School of Dentistry Faculty Research and Publications

A 26-year-old male presented with a 2-month history of a mildly painful, slow growing, nodular mass of the submental region. Examination revealed a 1.5 x 1.0-cm midline swelling of the submental region. The skin over the mass appeared normal. The mass was firm and slightly tender on palpation and appeared to be positioned within the submental space. Intraoral examination did not reveal any obvious pathologic findings and the nearby teeth showed no caries, periodontal disease, or mobility. The patient’s medical history was unremarkable.

Volume Analysis Of The Proximal Tibial Metaphysis, Kingsley Wang, Luis Eduardo Almeida, Alexis B. Olsson

School of Dentistry Faculty Research and Publications

Purpose

The Vitrea 2 imaging software (Vital Images Inc, Minnetonka, MN) was used for the volume analysis of the proximal tibial metaphysis.

Materials and Methods

Eighteen computed tomography scans of the proximal tibia were processed through the software, and 3-dimensional imaging of the proximal tibia was reconstructed.

Results

The volume and area of the proximal tibia that were generated resulted in a mean area of 127 cm² and a mean volume of 77.2 cm².

Conclusion

This study supports the use of the proximal tibial metaphysis as a source of low to moderate volume of autologous bone. When …

Response To A Retrospective Evaluation Of Crown-Fractured Permanent Teeth Treated In A Pediatric Dentistry Clinic, H. Cem Güngör

School of Dentistry Faculty Research and Publications

No abstract provided.

Physical Properties Of Denture Base Resins Potentially Resistant To Candida Adhesion, Gunjan Dhir, David W. Berzins, Virendra B. Dhuru, Periathamby A. Raj, Andrew R. Dentino

School of Dentistry Faculty Research and Publications

Purpose: The addition of anionic charge on denture base resins has been shown to inhibit Candida albicans adhesion and to facilitate adsorption of salivary defense molecules. The aim of this study was to evaluate the physical properties of a modified denture base resin for denture fabrication.

Materials and Methods: Specimens made from heat polymerizing resin Lucitone 199 were used as the control group. The two experimental groups, E-10 and E-20, had 10% and 20%, respectively, of the monomer substituted with an experimental phosphate-containing monomer. Flexural strength and modulus, water sorption, solubility, and color stability tests were conducted to ensure compliance …

Effect Of Various Curing Lights On The Degree Of Cure Of Orthodontic Adhesives, Markus Niepraschk, Christos Rahiotis, T. Gerard Bradley, Theodore Eliades, George Eliades

School of Dentistry Faculty Research and Publications

Introduction: The purpose of this study was to compare the percentage degree of cure (%DC) of orthodontic adhesive resins irradiated with 3 types of light sources of various intensities: plasma arc, halogen, and light-emitting diode (LED). Methods: Twenty maxillary incisor stainless steel brackets (0.018-in slot) were divided into 4 groups of 5 brackets each. A standardized amount of composite resin was applied to each bracket base, simulating its clinical application. The brackets were light-cured with a halogen light for 20 seconds (10 seconds each from the incisal and cervical bracket edges), an LED light for 20 seconds (10 seconds per …

Management Of Cervical Root Fracture Using Orthodontic Extrusion And Crown Reattachment: A Case Report, Harun Canoglu, H. Cem Güngör, Zafer C. Cehreli

School of Dentistry Faculty Research and Publications

Root fractures involve damage to pulp, cementum, dentin, and periodontal ligaments. These injuries affect 0.5% to 7% of permanent teeth. Cervical root fractures are less frequently seen and have a worse prognosis compared with the fractures in the apical or middle third of the root. This case report describes the treatment of a cervical root fracture in a maxillary central incisor. After removal of the coronal fragment, the root was filled temporarily with calcium hydroxide and orthodontic extrusion was initiated. The remaining root portion was elevated above the epithelial attachment and a successful coronal restoration was made using the natural …

A Retrospective Evaluation Of Crown-Fractured Permanent Teeth Treated In A Pediatric Dentistry Clinic, H. Cem Güngör, Serdar Uysal, Nil Altay

School of Dentistry Faculty Research and Publications

A retrospective study was carried out on the dental trauma records of 93 patients (55 boys, 38 girls) with 129 crown-fractured teeth. The patients’ average age was 9.57 years (SD 1.57), ranging between 7 and 15 years. Uncomplicated crown facture (comprising enamel–dentin) was the most observed type of injury (n = 107, 83%). Only 15 patients (16.13%) sought treatment in less than 24 h following the injury. Of 41 injured teeth (31.79%) the apices were open at the time of presentation at the clinic. The initial treatment of these injured teeth were interim restoration with acid-etch and composite (69%), …

Shear Bond Strength Comparison Between Two Orthodontic Adhesives And Self-Ligating And Conventional Brackets, Rodney G. Northrup, David W. Berzins, T. Gerard Bradley, William I. Schuckit

School of Dentistry Faculty Research and Publications

Objective: To evaluate and compare the shear bond strengths of two adhesives using two types of brackets: a conventional and a self-ligating bracket system.

Materials and Methods: Sixty extracted human premolars were collected. The premolars were randomly divided into three groups of 20 teeth. All three groups were direct bonded. Groups 1 and 2 used light-cured adhesive and primer (Transbond XT) with a conventional (Orthos) and a self-ligating bracket (Damon 2), respectively. Group 3 used a light-cured primer (Orthosolo) and a light-cured adhesive (Blūgloo) with a self-ligating bracket (Damon 2). The specimens were stored in distilled water at 37°C for …

Myopericytoma Of The Oral Cavity, Vivekanand Datta, Yeshwant B. Rawal, Harry H. Mincer, Mark K. Anderson

School of Dentistry Faculty Research and Publications

Background.

Myopericytoma is a rare mesenchymal neoplasm of pericytic cells demonstrating myoid differentiation. The lesion typically arises within the subcutaneous tissue of the extremities. We report a case that, to the best of our knowledge, is the first case of myopericytoma involving the soft tissue of the oral cavity.

Methods.

A 36-year-old woman had a 5-mm sessile, whitish-pink, firm tongue nodule. The patient underwent excisional biopsy, and histopathologic examination as well as immunohistochemical analysis were performed.

Results.

The differential diagnosis by histologic analysis included solitary fibrous tumor, myofibroma, glomus tumor, and myopericytoma. The results of immunohistochemical analysis, when combined with …

Root Fracture In Immature Tooth: Report Of A Case, H. Cem Güngör, Bülent Büyükgüral, Serdar Uysal

School of Dentistry Faculty Research and Publications

Root fracture injuries affect 0.5–7% of permanent teeth. Although this type of injury is rarely seen in teeth with immature root formation, the prognosis is generally good depending on the site of the fracture. A case report of horizontal root fracture in maxillary central incisor of an 8-year and 3-month-old girl and its treatment was presented.

Thermal Analysis Of As-Received And Clinically Retrieved Copper-Nickel-Titanium Orthodontic Archwires, Matthew C. Biermann, David W. Berzins, T. Gerard Bradley

School of Dentistry Faculty Research and Publications

Objective: To compare as-received copper-nickel-titanium (CuNiTi) archwires to those used in patients by means of differential scanning calorimetry (DSC). Also, the thermal or phase properties of 27°C, 35°C, and 40°C CuNiTi archwires were studied to ascertain if their properties match those indicated by the manufacturer.

Materials and Methods: Six wires of 27°C, 35°C, and 40°C CuNiTi were tested as-received, and six each of the 27°C and 35°C wires were examined after use in patients for an average of approximately 9 and 7 weeks, respectively. Segments of archwire were investigated by DSC over the temperature range from −100°C to 150°C at …

Traumatic Neuroma Following Sagittal Split Osteotomy Of The Mandible, R. H. Kallal, F. G. Ritto, Luis Eduardo Almeida, D. J. Crofton, G. P. Thomas

School of Dentistry Faculty Research and Publications

A 16-year-old male underwent bilateral sagittal split osteotomy of the mandible to correct a mandibular deficiency. Twenty-one years later, a routine panoramic radiograph revealed a radiolucent lesion on the left side of the mandible. The lesion was biopsied. As the patient did not have symptoms and the lesion was connected to the inferior alveolar nerve, the lesion was not totally excised in order to preserve nerve function. The histological features were consistent with traumatic neuroma, and no further surgical procedure was planned.

Immediate Surgical Repositioning Following Intrusive Luxation: A Case Report And Review Of The Literature, H. Cem Güngör, S. Burcak Cengiz, Nil Altay

School of Dentistry Faculty Research and Publications

This report presents a case of severe intrusive luxation of mature maxillary lateral incisor in a 10-year-old boy. The intruded tooth was immediately repositioned (surgical extrusion) and splinted within 2 h following injury. Tetracycline therapy was initiated at the time of repositioning and maintained for 10 days. Pulp removal and calcium hydroxide treatment of the root canal was carried out after repositioning. Splint was removed 1 month later. Definitive root canal treatment with gutta percha was accomplished at the second month recall. Clinical and radiographic examination 28 months after the surgical extrusion revealed satisfactory apical and periodontal healing.

Buccal And Palatal Talon Cusps With Pulp Extensions On A Supernumerary Primary Tooth, Esin Siraci, H. Cem Güngör, Zafer C. Cehreli, B. Taner

School of Dentistry Faculty Research and Publications

This paper reports an unusual occurrence of talon cusp on a supernumerary primary incisor, presenting on both labial and palatal sides. The tooth was scheduled for extraction due to its interference with the occlusion. Morphometric analysis of the taloned cusps was performed on digitized replicas of the tooth crown using open-source image analysis toolkit (ImageJ). Further non-destructive investigation of the taloned crown under cone-beam X-ray computed tomography revealed pulpal extensions in both talon cusps.

Bond Strength Of Direct And Indirect Bonded Brackets After Thermocycling, Jacob Daub, David W. Berzins, Brandon James Linn, T. Gerard Bradley

School of Dentistry Faculty Research and Publications

Thermocycling simulates the temperature dynamics in the oral environment. With direct bonding, thermocycling reduces the bond strength of orthodontic adhesives to tooth structure. The purpose of this study was to evaluate the shear bond strengths (SBS) of one direct and two indirect bonding methods/adhesives after thermocycling. Sixty human premolars were divided into three groups. Teeth in group 1 were bonded directly with Transbond XT. Teeth in group 2 were indirect bonded with Transbond XT/Sondhi Rapid Set, which is chemically cured. Teeth in group 3 were indirect bonded with Enlight LV/Orthosolo and light cured. Each sample was thermocycled between 5°C and …

A Comparison Of Bond Strength Between Direct- And Indirect-Bonding Methods, Brandon James Linn, David W. Berzins, Virendra B. Dhuru, T. Gerard Bradley

School of Dentistry Faculty Research and Publications

The purpose of this study was to evaluate and compare the shear bond strength and the sites of bond failure for brackets bonded to teeth, using two indirect-bonding material protocols and a direct-bonding technique. Sixty extracted human premolars were collected and randomly divided into three groups. The direct-bonded group (group 1) used a light-cured adhesive and primer (Transbond XT). One indirect-bonded group (group 2) consisted of a chemical-cured primer (Sondhi Rapid Set) and light-cured adhesive (Transbond XT), whereas the other group (group 3) used a light-cured primer (Orthosolo) and adhesive (Enlight LV). Forty hours after bonding, the samples were debonded. …

Force To Debond Brackets From High-Fusing And Low-Fusing Porcelain Systems, Nicholas P. Ferri, Theodore Eliades, Spiros Zinelis, T. Gerard Bradley

School of Dentistry Faculty Research and Publications

The purpose of this study was to test the hypothesis that porcelain surface finishing, ie, low- and high-fusing porcelain, has an effect on the amount of force required to debond orthodontic brackets. A total of 20 high-fusing and 20 low-fusing porcelain specimens were prepared, polished, and bonded with standard edgewise brackets using a suggested porcelain bonding protocol. The brackets were debonded with a universal testing machine at shear mode. Resin removal was performed using two methods: a multifluted carbide bur with and without the use of Sof-Lex polishing discs. Representative specimens were studied under a scanning electron microscope before and …

Porcelain Surface Roughness, Color And Gloss Changes After Orthodontic Bonding, Jacob Jarvis, Spiros Zinelis, Theodre Eliades, T. Gerard Bradley

School of Dentistry Faculty Research and Publications

The purpose of this study was to evaluate the alteration in surface characteristics after orthodontic debonding of two types of porcelain systems commonly used in prosthetic dentistry. For this purpose, porcelain specimens were fabricated from low-fusing (n = 20) and high-fusing (n = 20) porcelain. The baseline surface roughness, color, and gloss were evaluated using profilometry, color shade index, and gloss study. All specimens were bonded with brackets and debonded using a testing machine at a rate of 0.1 mm/minute crosshead speed. The porcelain surfaces were polished using a 12-fluted carbide composite removal bur (low-fusing, n = 20; high-fusing, n …

Gnathic Osteoblastoma: Clinicopathologic Review Of Seven Cases With Long-Term Follow-Up, Yeshwant B. Rawal, Francesca Angiero, Carl M. Allen, John R. Kalmar, Parish P. Sedghizadeh, Andrew M. Steinhilber

School of Dentistry Faculty Research and Publications

The clinical, radiographic and histopathologic features of seven cases of osteoblastoma of the jaw bones were analyzed. The mandible was involved in six cases and a periosteal presentation of the tumor was noted in three cases. Six tumors were symptomatic with pain being the common presentation. The radiographic appearance of the intraosseous tumors varied from well-defined radiolucencies to poorly-defined mixed lesions. A small periosteal tumor was undetectable radiographically. Histopathologically, the tumors exhibited variation in woven bone formation, osteoblastic rimming, lesional maturation as well as interaction of the tumor with surrounding host tissue. Intraosseous tumors were managed with aggressive surgical curettage …

Soft Tissue To Hard Tissue Advancement Ratios For Mandibular Elongation Using Distraction Osteogenesis In Children, Michael B. Melugin, Pamela R. Hanson, Christopher A. Bergstrom, William I. Schuckit, T. Gerard Bradley

School of Dentistry Faculty Research and Publications

Distraction osteogenesis is extensively used for the elongation of hypoplastic mandibles in children, yet the soft tissue profile response to this is not well understood. The pre- and posttreatment lateral cephalometric radiographs of 27 pediatric patients who underwent bilateral mandibular elongation using distraction osteogenesis were analyzed retrospectively to correlate horizontal soft tissue advancement with horizontal underlying bone advancement at B point and pogonion. Horizontal advancement (in millimeters) of bone and overlying soft tissue at these points was collected from the radiographs of each patient, and linear regression analysis was performed to determine the relationship of hard to soft tissue horizontal …

Clinical And Experimental Applications Of Nir-Led Photobiomodulation, Kristina D. Desmet, David A. Paz, Jesse J. Corry, Janis T. Eells, Margaret T. T. Wong-Riley, Michele M. Henry, Ellen V. Buchmann, Mary P. Connelly, Julia V. Dovi, Huan Ling Liang, Diane S. Henshel, Ronnie L. Yeager, Deborah S. Millsap, Jinhwan Lim, Lisa J. Gould, Rina Das, Marti Jett, Brian D. Hodgson, David Margolis, Harry T. Whelan

School of Dentistry Faculty Research and Publications

This review presents current research on the use of far-red to near-infrared (NIR) light treatment in various in vitro and in vivo models. Low-intensity light therapy, commonly referred to as “photobiomodulation,” uses light in the far-red to near-infrared region of the spectrum (630–1000 nm) and modulates numerous cellular functions. Positive effects of NIR–light-emitting diode (LED) light treatment include acceleration of wound healing, improved recovery from ischemic injury of the heart, and attenuated degeneration of injured optic nerves by improving mitochondrial energy metabolism and production. Various in vitro and in vivo models of mitochondrial dysfunction were treated with a variety of …

Spatially-Oriented Emr For Dental Surgery, Min Wu, Lisa J. Koenig, John Lynch, Thomas S. Wirtz

School of Dentistry Faculty Research and Publications

As digital dental images become widely available, a new Electronic MR system (EMR) will be critical for the success of applying new technology to dental care. This project is designed an image-based and spatially-oriented EMR for dental surgery. A new panoramic image-based annotation model will be developed, which will complement dental charting precisely locating specific spatial findings for each patient. A spatially-oriented, multilayered data model for dental EMR will be developed using Geographic Information System (GIS) methods. This project will explore the possibility of applying head and neck images from VHP into a spatially-oriented EMR system.

Suppression Of The Tumorigenic Phenotype In Human Oral Squamous Cell Carcinoma Cells By An Ethanol Extract Derived From Freeze-Dried Black Raspberries, Kapila A. Rodrigo, Yeshwant B. Rawal, Robert J. Renner, Steven J. Schwartz, Qingguo Tian, Peter R. Larsen, Susan R. Mallery

School of Dentistry Faculty Research and Publications

Despite focused efforts to improve therapy, 5-yr survival rates for persons with advanced-stage oral squamous cell carcinoma (SCC) remain discouragingly low. Clearly, early detection combined with strategies for local intervention, such as chemoprevention prior to SCC development, could dramatically improve clinical outcomes. Previously conducted oral cavity human chemoprevention trials, however, have provided mixed results. Although some therapies showed efficacy, they were often accompanied by either significant toxicities or circulating antiadenoviral antibodies. It is clearly apparent that identification of nontoxic, effective treatments is essential to prevent malignant transformation of oral epithelial dysplasias. This study employed cell lines isolated from human oral …